ID   GLTD_MYCTU              Reviewed;         488 AA.
AC   P9WN19; F2GDM0; L0TFG6; P96219; Q7D4Q4;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Glutamate synthase [NADPH] small chain;
DE            EC=1.4.1.13;
DE   AltName: Full=Glutamate synthase subunit beta;
DE            Short=GLTS beta chain;
GN   Name=gltD; OrderedLocusNames=Rv3858c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + L-glutamine
CC         + NADPH; Xref=Rhea:RHEA:15501, ChEBI:CHEBI:15378, ChEBI:CHEBI:16810,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:58359; EC=1.4.1.13;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-glutamate biosynthesis via GLT
CC       pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+)
CC       route): step 1/1.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
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DR   EMBL; AL123456; CCP46687.1; -; Genomic_DNA.
DR   PIR; G70655; G70655.
DR   RefSeq; NP_218375.1; NC_000962.3.
DR   RefSeq; WP_003899732.1; NZ_NVQJ01000057.1.
DR   AlphaFoldDB; P9WN19; -.
DR   SMR; P9WN19; -.
DR   STRING; 83332.Rv3858c; -.
DR   PaxDb; P9WN19; -.
DR   DNASU; 886196; -.
DR   GeneID; 45427862; -.
DR   GeneID; 886196; -.
DR   KEGG; mtu:Rv3858c; -.
DR   TubercuList; Rv3858c; -.
DR   eggNOG; COG0493; Bacteria.
DR   OMA; DCVGTAH; -.
DR   PhylomeDB; P9WN19; -.
DR   UniPathway; UPA00634; UER00689.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron transfer activity; IBA:GO_Central.
DR   GO; GO:0004355; F:glutamate synthase (NADPH) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009061; P:anaerobic respiration; IBA:GO_Central.
DR   GO; GO:0097054; P:L-glutamate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.1060.10; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   InterPro; IPR028261; DPD_II.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR006005; Glut_synth_ssu1.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   Pfam; PF14691; Fer4_20; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   TIGRFAMs; TIGR01317; GOGAT_sm_gam; 1.
PE   1: Evidence at protein level;
KW   4Fe-4S; Amino-acid biosynthesis; Glutamate biosynthesis; Iron; Iron-sulfur;
KW   Metal-binding; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..488
FT                   /note="Glutamate synthase [NADPH] small chain"
FT                   /id="PRO_0000419546"
FT   DOMAIN          38..69
FT                   /note="4Fe-4S ferredoxin-type"
SQ   SEQUENCE   488 AA;  53452 MW;  22455022F7EF0D9F CRC64;
     MADPGGFLKY THRKLPKRRP VPLRLRDWRE VYEEFDNESL RQQATRCMDC GIPFCHNGCP
     LGNLIPEWND LVRRGRWRDA IERLHATNNF PDFTGRLCPA PCEPACVLGI NQDPVTIKQI
     ELEIIDKAFD EGWVQPRPPR KLTGQTVAVV GSGPAGLAAA QQLTRAGHTV TVFEREDRIG
     GLLRYGIPEF KMEKRHLDRR LDQMRSEGTE FRPGVNVGVD ISAEKLRADF DAVVLAGGAT
     AWRELPIPGR ELEGVHQAME FLPWANRVQE GDDVLDEDGQ PPITAKGKKV VIIGGGDTGA
     DCLGTVHRQG AIAVHQFEIM PRPPDARAES TPWPTYPLMY RVSAAHEEGG ERVFSVNTEA
     FVGTDGRVSA LRAHEVTMLD GKFVKVEGSD FELEADLVLL AMGFVGPERA GLLTDLGVKF
     TERGNVARGD DFDTSVPGVF VAGDMGRGQS LIVWAIAEGR AAAAAVDRYL MGSSALPAPV
     KPTAAPLQ