GLTR_PSEAE
ID GLTR_PSEAE Reviewed; 242 AA.
AC G3XCY6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Transcriptional regulatory protein GltR {ECO:0000305};
GN Name=gltR {ECO:0000303|PubMed:8830708};
GN OrderedLocusNames=PA3192 {ECO:0000312|EMBL:AAG06580.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8830708; DOI=10.1128/jb.178.20.6064-6066.1996;
RA Sage A.E., Proctor W.D., Phibbs P.V. Jr.;
RT "A two-component response regulator, gltR, is required for glucose
RT transport activity in Pseudomonas aeruginosa PAO1.";
RL J. Bacteriol. 178:6064-6066(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=22262100; DOI=10.1099/mic.0.056127-0;
RA O'Callaghan J., Reen F.J., Adams C., Casey P.G., Gahan C.G.M., O'Gara F.;
RT "A novel host-responsive sensor mediates virulence and type III secretion
RT during Pseudomonas aeruginosa-host cell interactions.";
RL Microbiology 158:1057-1070(2012).
RN [4]
RP FUNCTION, DNA-BINDING, ACTIVITY REGULATION, PROBABLE PHOSPHORYLATION AT
RP ASP-56, AND MUTAGENESIS OF ASP-56.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=24920832; DOI=10.1093/nar/gku496;
RA Daddaoua A., Molina-Santiago C., de la Torre J., Krell T., Ramos J.L.;
RT "GtrS and GltR form a two-component system: the central role of 2-
RT ketogluconate in the expression of exotoxin A and glucose catabolic enzymes
RT in Pseudomonas aeruginosa.";
RL Nucleic Acids Res. 42:7654-7663(2014).
CC -!- FUNCTION: Member of the two-component regulatory system GtrS/GltR
CC involved in the regulation of glucose metabolism and transport, as well
CC as regulation of the exotoxin A gene expression (PubMed:24920832). GltR
CC controls the transcription of genes involved in glucose metabolism (glk
CC and edd/gap-1) and transport (oprB) as well as the expression of toxA
CC that encodes exotoxin A, the primary virulence factor (PubMed:8830708,
CC PubMed:24920832). Acts as a repressor that is released from its target
CC operators upon phosphorylation (PubMed:24920832).
CC {ECO:0000269|PubMed:24920832, ECO:0000269|PubMed:8830708}.
CC -!- FUNCTION: Contributes to modulation of the type III secretion system
CC (T3SS) in response to host cells via the regulation of the OprB
CC transport system. {ECO:0000269|PubMed:22262100}.
CC -!- ACTIVITY REGULATION: Phosphorylation of GltR induces its dissociation
CC from DNA leading to transcriptional activation.
CC {ECO:0000269|PubMed:24920832}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by GtrS. {ECO:0000269|PubMed:24920832}.
CC -!- DISRUPTION PHENOTYPE: Disruption of the gene causes loss of glucose
CC transport activity (PubMed:8830708). Inactivation results in elevated
CC ingestion and reduced cytotoxicity of eukaryotic cells during
CC P.aeruginosa infection (PubMed:22262100). {ECO:0000269|PubMed:22262100,
CC ECO:0000269|PubMed:8830708}.
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DR EMBL; AE004091; AAG06580.1; -; Genomic_DNA.
DR PIR; E83246; E83246.
DR RefSeq; NP_251882.1; NC_002516.2.
DR RefSeq; WP_003091475.1; NZ_QZGE01000019.1.
DR AlphaFoldDB; G3XCY6; -.
DR SMR; G3XCY6; -.
DR STRING; 287.DR97_4744; -.
DR PaxDb; G3XCY6; -.
DR EnsemblBacteria; AAG06580; AAG06580; PA3192.
DR GeneID; 882907; -.
DR KEGG; pae:PA3192; -.
DR PATRIC; fig|208964.12.peg.3338; -.
DR PseudoCAP; PA3192; -.
DR HOGENOM; CLU_000445_30_4_6; -.
DR InParanoid; G3XCY6; -.
DR OMA; FSLCRWV; -.
DR PhylomeDB; G3XCY6; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0010828; P:positive regulation of glucose transmembrane transport; IMP:PseudoCAP.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Two-component regulatory system.
FT CHAIN 1..242
FT /note="Transcriptional regulatory protein GltR"
FT /id="PRO_0000454757"
FT DOMAIN 7..123
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 134..234
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 56
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169,
FT ECO:0000305|PubMed:24920832"
FT MUTAGEN 56
FT /note="D->A: Cannot dissociate from DNA."
FT /evidence="ECO:0000269|PubMed:24920832"
SQ SEQUENCE 242 AA; 27413 MW; 54106B8DB26D799E CRC64;
MSANGRSILL VDDDQEIREL LETYLSRAGF QVRSVSRGAD FRQALCEEEA SLAILDVMLP
DEDGFSLCRW IRSHQRLACM PIIMLTASSD EADRVIGLEL GADDYLGKPF SPRELLARIK
ALLRRAQFTQ VRGGDVLAFE DWRLDTVSHR LFHEDGEEFF LSGADFALLK LFLDHPQQIL
DRDTIANATR GREVLPLERI VDMAVSRLRQ RLRDTGKAPR LIQTVRGSGY LLAAQVRPHL
QP