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GLTR_PSEAE
ID   GLTR_PSEAE              Reviewed;         242 AA.
AC   G3XCY6;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Transcriptional regulatory protein GltR {ECO:0000305};
GN   Name=gltR {ECO:0000303|PubMed:8830708};
GN   OrderedLocusNames=PA3192 {ECO:0000312|EMBL:AAG06580.1};
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=8830708; DOI=10.1128/jb.178.20.6064-6066.1996;
RA   Sage A.E., Proctor W.D., Phibbs P.V. Jr.;
RT   "A two-component response regulator, gltR, is required for glucose
RT   transport activity in Pseudomonas aeruginosa PAO1.";
RL   J. Bacteriol. 178:6064-6066(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=22262100; DOI=10.1099/mic.0.056127-0;
RA   O'Callaghan J., Reen F.J., Adams C., Casey P.G., Gahan C.G.M., O'Gara F.;
RT   "A novel host-responsive sensor mediates virulence and type III secretion
RT   during Pseudomonas aeruginosa-host cell interactions.";
RL   Microbiology 158:1057-1070(2012).
RN   [4]
RP   FUNCTION, DNA-BINDING, ACTIVITY REGULATION, PROBABLE PHOSPHORYLATION AT
RP   ASP-56, AND MUTAGENESIS OF ASP-56.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=24920832; DOI=10.1093/nar/gku496;
RA   Daddaoua A., Molina-Santiago C., de la Torre J., Krell T., Ramos J.L.;
RT   "GtrS and GltR form a two-component system: the central role of 2-
RT   ketogluconate in the expression of exotoxin A and glucose catabolic enzymes
RT   in Pseudomonas aeruginosa.";
RL   Nucleic Acids Res. 42:7654-7663(2014).
CC   -!- FUNCTION: Member of the two-component regulatory system GtrS/GltR
CC       involved in the regulation of glucose metabolism and transport, as well
CC       as regulation of the exotoxin A gene expression (PubMed:24920832). GltR
CC       controls the transcription of genes involved in glucose metabolism (glk
CC       and edd/gap-1) and transport (oprB) as well as the expression of toxA
CC       that encodes exotoxin A, the primary virulence factor (PubMed:8830708,
CC       PubMed:24920832). Acts as a repressor that is released from its target
CC       operators upon phosphorylation (PubMed:24920832).
CC       {ECO:0000269|PubMed:24920832, ECO:0000269|PubMed:8830708}.
CC   -!- FUNCTION: Contributes to modulation of the type III secretion system
CC       (T3SS) in response to host cells via the regulation of the OprB
CC       transport system. {ECO:0000269|PubMed:22262100}.
CC   -!- ACTIVITY REGULATION: Phosphorylation of GltR induces its dissociation
CC       from DNA leading to transcriptional activation.
CC       {ECO:0000269|PubMed:24920832}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by GtrS. {ECO:0000269|PubMed:24920832}.
CC   -!- DISRUPTION PHENOTYPE: Disruption of the gene causes loss of glucose
CC       transport activity (PubMed:8830708). Inactivation results in elevated
CC       ingestion and reduced cytotoxicity of eukaryotic cells during
CC       P.aeruginosa infection (PubMed:22262100). {ECO:0000269|PubMed:22262100,
CC       ECO:0000269|PubMed:8830708}.
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DR   EMBL; AE004091; AAG06580.1; -; Genomic_DNA.
DR   PIR; E83246; E83246.
DR   RefSeq; NP_251882.1; NC_002516.2.
DR   RefSeq; WP_003091475.1; NZ_QZGE01000019.1.
DR   AlphaFoldDB; G3XCY6; -.
DR   SMR; G3XCY6; -.
DR   STRING; 287.DR97_4744; -.
DR   PaxDb; G3XCY6; -.
DR   EnsemblBacteria; AAG06580; AAG06580; PA3192.
DR   GeneID; 882907; -.
DR   KEGG; pae:PA3192; -.
DR   PATRIC; fig|208964.12.peg.3338; -.
DR   PseudoCAP; PA3192; -.
DR   HOGENOM; CLU_000445_30_4_6; -.
DR   InParanoid; G3XCY6; -.
DR   OMA; FSLCRWV; -.
DR   PhylomeDB; G3XCY6; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0010828; P:positive regulation of glucose transmembrane transport; IMP:PseudoCAP.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome; Repressor;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..242
FT                   /note="Transcriptional regulatory protein GltR"
FT                   /id="PRO_0000454757"
FT   DOMAIN          7..123
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        134..234
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         56
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169,
FT                   ECO:0000305|PubMed:24920832"
FT   MUTAGEN         56
FT                   /note="D->A: Cannot dissociate from DNA."
FT                   /evidence="ECO:0000269|PubMed:24920832"
SQ   SEQUENCE   242 AA;  27413 MW;  54106B8DB26D799E CRC64;
     MSANGRSILL VDDDQEIREL LETYLSRAGF QVRSVSRGAD FRQALCEEEA SLAILDVMLP
     DEDGFSLCRW IRSHQRLACM PIIMLTASSD EADRVIGLEL GADDYLGKPF SPRELLARIK
     ALLRRAQFTQ VRGGDVLAFE DWRLDTVSHR LFHEDGEEFF LSGADFALLK LFLDHPQQIL
     DRDTIANATR GREVLPLERI VDMAVSRLRQ RLRDTGKAPR LIQTVRGSGY LLAAQVRPHL
     QP
 
 
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