GLT_DROME
ID GLT_DROME Reviewed; 1026 AA.
AC P33438; A4V0G5; Q9VLJ3;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Glutactin;
DE Flags: Precursor;
GN Name=Glt; ORFNames=CG9280;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND SULFATION.
RC STRAIN=Oregon-R;
RX PubMed=2108864; DOI=10.1002/j.1460-2075.1990.tb08229.x;
RA Olson P.F., Fessler L.I., Nelson R.E., Sterne R.E., Campbell A.G.,
RA Fessler J.H.;
RT "Glutactin, a novel Drosophila basement membrane-related glycoprotein with
RT sequence similarity to serine esterases.";
RL EMBO J. 9:1219-1227(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Not known. Binds calcium ions.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix, basement membrane.
CC -!- PTM: Extensively O-glycosylated and also N-glycosylated.
CC -!- PTM: About four tyrosines are sulfated. {ECO:0000269|PubMed:2108864}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the type-B
CC carboxylesterase/lipase family. {ECO:0000305}.
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DR EMBL; X53286; CAA37380.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF52697.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10678.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10679.1; -; Genomic_DNA.
DR EMBL; AY069776; AAL39921.1; -; mRNA.
DR PIR; S12519; S12519.
DR RefSeq; NP_001245946.1; NM_001259017.2.
DR RefSeq; NP_477504.1; NM_058156.4.
DR AlphaFoldDB; P33438; -.
DR SMR; P33438; -.
DR BioGRID; 60312; 46.
DR IntAct; P33438; 3.
DR STRING; 7227.FBpp0079329; -.
DR ESTHER; drome-gluta; Glutactin.
DR GlyGen; P33438; 4 sites.
DR PaxDb; P33438; -.
DR PRIDE; P33438; -.
DR DNASU; 34193; -.
DR EnsemblMetazoa; FBtr0079727; FBpp0079331; FBgn0001114.
DR EnsemblMetazoa; FBtr0307091; FBpp0297934; FBgn0001114.
DR GeneID; 34193; -.
DR KEGG; dme:Dmel_CG9280; -.
DR UCSC; CG9280-RA; d. melanogaster.
DR CTD; 34193; -.
DR FlyBase; FBgn0001114; Glt.
DR VEuPathDB; VectorBase:FBgn0001114; -.
DR eggNOG; KOG1516; Eukaryota.
DR GeneTree; ENSGT00940000164971; -.
DR HOGENOM; CLU_295824_0_0_1; -.
DR InParanoid; P33438; -.
DR OMA; YNPYANW; -.
DR OrthoDB; 754103at2759; -.
DR PhylomeDB; P33438; -.
DR SignaLink; P33438; -.
DR BioGRID-ORCS; 34193; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34193; -.
DR PRO; PR:P33438; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0001114; Expressed in arthropod fat body and 14 other tissues.
DR ExpressionAtlas; P33438; baseline and differential.
DR Genevisible; P33438; DM.
DR GO; GO:0005604; C:basement membrane; IDA:FlyBase.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0031983; C:vesicle lumen; IDA:FlyBase.
DR GO; GO:0098542; P:defense response to other organism; IMP:FlyBase.
DR GO; GO:0042381; P:hemolymph coagulation; IDA:FlyBase.
DR GO; GO:0008045; P:motor neuron axon guidance; IMP:FlyBase.
DR GO; GO:0016201; P:synaptic target inhibition; IMP:FlyBase.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 1: Evidence at protein level;
KW Basement membrane; Calcium; Direct protein sequencing; Disulfide bond;
KW Extracellular matrix; Glycoprotein; Reference proteome; Secreted; Signal;
KW Sulfation.
FT SIGNAL 1..17
FT CHAIN 18..1026
FT /note="Glutactin"
FT /id="PRO_0000008639"
FT REGION 601..641
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 659..695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 723..1026
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 601..636
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 723..754
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 807..855
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 867..895
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 896..914
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 923..945
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 946..971
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 987..1026
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 26
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 29
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 182
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 559
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 645
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 727
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 836
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 862
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 865
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 868
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 922
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 928
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 981
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 984
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT MOD_RES 1006
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000255"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 402
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 810
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 123..145
FT /evidence="ECO:0000250"
FT DISULFID 298..316
FT /evidence="ECO:0000250"
FT CONFLICT 919..921
FT /note="Missing (in Ref. 1; CAA37380)"
FT /evidence="ECO:0000305"
FT CONFLICT 961
FT /note="S -> L (in Ref. 1; CAA37380)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1026 AA; 118749 MW; EEB11216C4DC9DF6 CRC64;
MKPLLLVLAL CGAQVHAHSV GLRPDYNDYS DEDTRRDWLP EPLKPVPWQS ETRYAQPQEA
VVQAPEVGQI LGISGHKTIA NRPVNAFLGI RYGTVGGGLA RFQAAQPIGY QGRVNATVQS
PNCAQFPELD RLRLSESRGE NVDDCLTLDI YAPEGANQLP VLVFVHGEML FDGGSEEAQP
DYVLEKDVLL VSINYRLAPF GFLSALTDEL PGNVALSDLQ LALEWLQRNV VHFGGNAGQV
TLVGQAGGAT LAHALSLSGR AGNLFQQLIL QSGTALNPYL IDNQPLDTLS TFARLARCPP
PSINPSAQGL KPLYDCLARL PTSQLVAAFE QLLLQNEHLG LTQLGGFKLV VGDPLGFLPS
HPASLATNSS LALPMIIGAT KDASAFIVSR IYDQLARLQS RNVSDYIDVV LRHTAPPSEH
RLWKQWALRE IFTPIQEQTA SLQTVAPGLL ELSNYILYRA PVINSISQSY RSVPAYLYTF
DYRGEHHRFG HLSNPLPFGV DASLSDDSVY LFPYPPEASR LNPLDRSLSR ALVTMWVNFA
TTGVPNPSSG VWPQATSEYG PFLRFTNNQQ SPLELDPHFG EGIYLPNYRV IYKPTTNFSP
PITTTTTTTT TTTTTSRPYA YNPYANWQNR PSQQHPNWHP ADPEYVRAQE ARQQEFIRER
EQRRREQQLR DQQRYPQQEP REQQDERIRQ QREQEERLRQ QREQEERLRQ QRELEERIRQ
QQEREQYERE QQEREQRERE ELERQQRERE QQQPEQQPEY NPEPVNPWGY PVQEPQPDDN
PEDGRLPYPS YEQYGPEGNE NLPETDANRN FSEEDREQQQ QEQLRREQQE QQEREYQLQL
EREQQEREQQ ERGQQEPGPE EYPSYEEYSR ALQEKNAERD RIYAEEQERE RQQQETLLQE
NQQHPEQSLP EEQPTHPNYE AYDGDRSYAE EQEREQQRRD QVEQEREEQP DEDQGEEYER
SPDEEEAAEQ DVLKVEDFPS YEAYLEAATK LREEQEEQEK LEEERYRAQQ EEEDRIQAER
ERNSRN
