ID   AMIE_STRPN              Reviewed;         355 AA.
AC   P0A2U8; P18765;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Oligopeptide transport ATP-binding protein AmiE;
GN   Name=amiE; OrderedLocusNames=SP_1888;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=R6 / R800;
RX   PubMed=2352474; DOI=10.1111/j.1365-2958.1990.tb00632.x;
RA   Alloing G., Trombe M.C., Claverys J.-P.;
RT   "The ami locus of the Gram-positive bacterium Streptococcus pneumoniae is
RT   similar to binding protein-dependent transport operons of Gram-negative
RT   bacteria.";
RL   Mol. Microbiol. 4:633-644(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- FUNCTION: Part of the binding-protein-dependent transport system for
CC       oligopeptides. Probably responsible for energy coupling to the
CC       transport system.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR   EMBL; X17337; CAA35216.1; -; Genomic_DNA.
DR   EMBL; AE005672; AAK75959.1; -; Genomic_DNA.
DR   PIR; F95220; F95220.
DR   PIR; S11152; S11152.
DR   RefSeq; WP_000159554.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; P0A2U8; -.
DR   SMR; P0A2U8; -.
DR   STRING; 170187.SP_1888; -.
DR   EnsemblBacteria; AAK75959; AAK75959; SP_1888.
DR   GeneID; 60232926; -.
DR   KEGG; spn:SP_1888; -.
DR   eggNOG; COG0444; Bacteria.
DR   OMA; LDPINTI; -.
DR   PhylomeDB; P0A2U8; -.
DR   BioCyc; SPNE170187:G1FZB-1918-MON; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR   GO; GO:0015833; P:peptide transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR013563; Oligopep_ABC_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF08352; oligo_HPY; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01727; oligo_HPY; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Peptide transport; Protein transport; Transport.
FT   CHAIN           1..355
FT                   /note="Oligopeptide transport ATP-binding protein AmiE"
FT                   /id="PRO_0000091927"
FT   DOMAIN          9..260
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         45..52
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   CONFLICT        59
FT                   /note="T -> R (in Ref. 1; CAA35216)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        70
FT                   /note="Q -> E (in Ref. 1; CAA35216)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   355 AA;  39490 MW;  C26CCB2F1D35BC52 CRC64;
     MTKEKNVILT ARDIVVEFDV RDKVLTAIRG VSLELVEGEV LALVGESGSG KSVLTKTFTG
     MLEENGRIAQ GSIDYRGQDL TALSSHKDWE QIRGAKIATI FQDPMTSLDP IKTIGSQITE
     VIVKHQGKTA KEAKELAIDY MNKVGIPDAD RRFNEYPFQY SGGMRQRIVI AIALACRPDV
     LICDEPTTAL DVTIQAQIID LLKSLQNEYH FTTIFITHDL GVVASIADKV AVMYAGEIVE
     YGTVEEVFYD PRHPYTWSLL SSLPQLADDK GDLYSIPGTP PSLYTDLKGD AFALRSDYAM
     QIDFEQKAPQ FSVSETHWAK TWLLHEDAPK VEKPAVIANL HDKIREKMGF AHLAD