GLU2_MAIZE
ID GLU2_MAIZE Reviewed; 223 AA.
AC P04706; P02860; P08415;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 25-MAY-2022, entry version 135.
DE RecName: Full=Glutelin-2;
DE AltName: Full=27 kDa zein;
DE AltName: Full=Alcohol-soluble reduced glutelin;
DE Short=ASG;
DE AltName: Full=Zein Zc2;
DE AltName: Full=Zein-gamma;
DE Flags: Precursor;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. W64 X W64O2;
RX PubMed=3596247; DOI=10.1016/0378-1119(87)90393-3;
RA Prat S., Perez-Grau L., Puigdomenech P.;
RT "Multiple variability in the sequence of a family of maize endosperm
RT proteins.";
RL Gene 52:41-49(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. inbred line E-10;
RX PubMed=3839076; DOI=10.1093/nar/13.5.1493;
RA Prat S., Cortadas J., Puigdomenech P., Palau J.;
RT "Nucleic acid (cDNA) and amino acid sequences of the maize endosperm
RT protein glutelin-2.";
RL Nucleic Acids Res. 13:1493-1504(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RA Boronat A., Martinez M.C., Reina M., Puigdomenech P., Palau J.;
RT "Isolation and sequencing of a 28 kd glutelin-2 gene from maize: common
RT elements in the 5' flanking regions among zein and glutelin genes.";
RL Plant Sci. 47:95-102(1986).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Wisconsin 64A; TISSUE=Endosperm;
RX PubMed=2243788; DOI=10.1093/nar/18.21.6426;
RA Reina M., Ponte I., Guillen P., Boronat A., Palau J.;
RT "Sequence analysis of a genomic clone encoding a Zc2 protein from Zea mays
RT W64 A.";
RL Nucleic Acids Res. 18:6426-6426(1990).
RN [5]
RP NUCLEOTIDE SEQUENCE OF 15-223, AND VARIANT SER-28.
RX PubMed=16664810; DOI=10.1104/pp.81.1.70;
RA Wang S.-Z., Esen A.;
RT "Primary structure of a proline-rich zein and its cDNA.";
RL Plant Physiol. 81:70-74(1986).
RN [6]
RP PROTEIN SEQUENCE OF 20-77.
RA Esen A., Bietz J.A., Paulis J.W., Wall J.S.;
RT "Tandem repeats in the N-terminal sequence of a proline-rich protein from
RT corn endosperm.";
RL Nature 296:678-679(1982).
CC -!- FUNCTION: Seed storage protein. It accounts for about 15% of the total
CC endosperm protein content.
CC -!- SUBCELLULAR LOCATION: Vacuole, aleurone grain membrane. Note=Border of
CC the inner part of the membrane of endosperm protein bodies.
CC -!- SIMILARITY: Belongs to the gliadin/glutenin family. {ECO:0000305}.
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DR EMBL; X02230; CAA26149.1; -; mRNA.
DR EMBL; M16066; AAA33468.1; -; Genomic_DNA.
DR EMBL; M16218; AAA33537.1; -; mRNA.
DR EMBL; X53514; CAA37594.1; -; Genomic_DNA.
DR EMBL; S78780; AAP32017.1; -; mRNA.
DR PIR; A03351; ZMZM5.
DR PIR; A93557; ZMZM19.
DR RefSeq; NP_001105354.1; NM_001111884.1.
DR AlphaFoldDB; P04706; -.
DR STRING; 4577.GRMZM2G138727_P01; -.
DR Allergome; 2780; Zea m 27kD Zein.
DR PaxDb; P04706; -.
DR EnsemblPlants; Zm00001eb313800_T001; Zm00001eb313800_P001; Zm00001eb313800.
DR Gramene; Zm00001eb313800_T001; Zm00001eb313800_P001; Zm00001eb313800.
DR MaizeGDB; 58111; -.
DR eggNOG; ENOG502R4K8; Eukaryota.
DR HOGENOM; CLU_1477240_0_0_1; -.
DR OMA; VEPQHRY; -.
DR Proteomes; UP000007305; Chromosome 7.
DR ExpressionAtlas; P04706; baseline and differential.
DR Genevisible; P04706; ZM.
DR GO; GO:0032578; C:aleurone grain membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-KW.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR001954; Glia_glutenin.
DR InterPro; IPR000480; Glutelin.
DR PANTHER; PTHR33454; PTHR33454; 2.
DR Pfam; PF13016; Gliadin; 1.
DR PRINTS; PR00211; GLUTELIN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Reference proteome; Repeat;
KW Seed storage protein; Signal; Storage protein; Vacuole.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|Ref.6"
FT CHAIN 20..223
FT /note="Glutelin-2"
FT /id="PRO_0000032214"
FT REPEAT 31..36
FT /note="1-1"
FT REPEAT 37..42
FT /note="1-2"
FT REPEAT 43..48
FT /note="1-3"
FT REPEAT 49..54
FT /note="1-4"
FT REPEAT 55..60
FT /note="1-5"
FT REPEAT 61..66
FT /note="1-6"
FT REPEAT 67..72
FT /note="1-7"
FT REPEAT 73..78
FT /note="1-8"
FT REPEAT 97..104
FT /note="2-1"
FT REPEAT 105..112
FT /note="2-2"
FT REGION 31..78
FT /note="8 X 6 AA tandem repeats of P-P-P-V-H-L"
FT REGION 97..112
FT /note="2 X 8 AA tandem repeats of Q-P-H-P-C-P-C-Q"
FT VARIANT 28
FT /note="C -> S (in 66% of the molecules)"
FT /evidence="ECO:0000269|PubMed:16664810"
SQ SEQUENCE 223 AA; 23689 MW; 3E80C06B2DB58372 CRC64;
MRVLLVALAL LALAASATST HTSGGCGCQP PPPVHLPPPV HLPPPVHLPP PVHLPPPVHL
PPPVHLPPPV HVPPPVHLPP PPCHYPTQPP RPQPHPQPHP CPCQQPHPSP CQLQGTCGVG
STPILGQCVE FLRHQCSPTA TPYCSPQCQS LRQQCCQQLR QVEPQHRYQA IFGLVLQSIL
QQQPQSGQVA GLLAAQIAQQ LTAMCGLQQP TPCPYAAAGG VPH
