GLV11_ARATH
ID GLV11_ARATH Reviewed; 116 AA.
AC Q3E880; A0MFQ8; B3H4B2; E5RQK8; Q1PDH0;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Protein GOLVEN 11 {ECO:0000303|PubMed:23370719};
DE AltName: Full=CLAVATA3/ESR (CLE)-related protein CLEL8 {ECO:0000303|PubMed:22307643};
DE Short=CLE-Like protein 8 {ECO:0000303|PubMed:22307643};
DE AltName: Full=Root meristem growth factor 1 {ECO:0000303|PubMed:20798316};
DE Short=AtRGF1 {ECO:0000303|PubMed:20798316};
DE Contains:
DE RecName: Full=GLV11p {ECO:0000303|PubMed:23370719};
DE AltName: Full=RGF1p {ECO:0000303|PubMed:20798316};
DE Flags: Precursor;
GN Name=GLV11 {ECO:0000303|PubMed:23370719};
GN Synonyms=CLEL8 {ECO:0000303|PubMed:22307643},
GN RGF1 {ECO:0000303|PubMed:20798316};
GN OrderedLocusNames=At5g60810 {ECO:0000312|Araport:AT5G60810};
GN ORFNames=MAE1.14 {ECO:0000312|EMBL:AB015472};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PROTEIN SEQUENCE OF 104-116,
RP IDENTIFICATION BY MASS SPECTROMETRY, SULFATION AT TYR-105, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY AUXIN, DISRUPTION
RP PHENOTYPE, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=20798316; DOI=10.1126/science.1191132;
RA Matsuzaki Y., Ogawa-Ohnishi M., Mori A., Matsubayashi Y.;
RT "Secreted peptide signals required for maintenance of root stem cell niche
RT in Arabidopsis.";
RL Science 329:1065-1067(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP FUNCTION, NOMENCLATURE, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=22307643; DOI=10.1073/pnas.1119864109;
RA Meng L., Buchanan B.B., Feldman L.J., Luan S.;
RT "CLE-like (CLEL) peptides control the pattern of root growth and lateral
RT root development in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:1760-1765(2012).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=23370719; DOI=10.1104/pp.112.206029;
RA Fernandez A., Drozdzecki A., Hoogewijs K., Nguyen A., Beeckman T.,
RA Madder A., Hilson P.;
RT "Transcriptional and functional classification of the GOLVEN/ROOT GROWTH
RT FACTOR/CLE-like signaling peptides reveals their role in lateral root and
RT hair formation.";
RL Plant Physiol. 161:954-970(2013).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY PHOSPHATE-DEPRIVATION.
RC STRAIN=cv. Columbia;
RX PubMed=25856240; DOI=10.1111/nph.13405;
RA Cederholm H.M., Benfey P.N.;
RT "Distinct sensitivities to phosphate deprivation suggest that RGF peptides
RT play disparate roles in Arabidopsis thaliana root development.";
RL New Phytol. 207:683-691(2015).
RN [8]
RP INTERACTION WITH RGI1.
RC STRAIN=cv. Columbia;
RX PubMed=27229312; DOI=10.1038/cr.2016.63;
RA Ou Y., Lu X., Zi Q., Xun Q., Zhang J., Wu Y., Shi H., Wei Z., Zhao B.,
RA Zhang X., He K., Gou X., Li C., Li J.;
RT "RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are
RT essential for the perception of root meristem growth factor 1 in
RT Arabidopsis thaliana.";
RL Cell Res. 26:686-698(2016).
RN [9]
RP INTERACTION WITH RGI1; RGI2 AND RGI3.
RC STRAIN=cv. Columbia;
RX PubMed=27001831; DOI=10.1073/pnas.1522639113;
RA Shinohara H., Mori A., Yasue N., Sumida K., Matsubayashi Y.;
RT "Identification of three LRR-RKs involved in perception of root meristem
RT growth factor in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:3897-3902(2016).
RN [10]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=31801996; DOI=10.1038/s41586-019-1819-6;
RA Yamada M., Han X., Benfey P.N.;
RT "RGF1 controls root meristem size through ROS signalling.";
RL Nature 577:85-88(2020).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 104-116, AND INTERACTION WITH
RP RGI3/RGFR1 AND RGI4/RGFR2/SKM2.
RC STRAIN=cv. Columbia;
RX PubMed=27229311; DOI=10.1038/cr.2016.62;
RA Song W., Liu L., Wang J., Wu Z., Zhang H., Tang J., Lin G., Wang Y.,
RA Wen X., Li W., Han Z., Guo H., Chai J.;
RT "Signature motif-guided identification of receptors for peptide hormones
RT essential for root meristem growth.";
RL Cell Res. 26:674-685(2016).
CC -!- FUNCTION: [GLV11p]: Acts as a peptide hormone recognized by receptors
CC (e.g. RGI1 and RGI2) to trigger signaling events including the
CC regulation of RITF1 expression and leading to the production of
CC reactive oxygen species (ROS) in roots to modulate meristem size
CC (PubMed:31801996). Signaling peptide (root growth factor) that
CC maintains the postembryonic root stem cell niche in a PIN2-traffic
CC dependent manner by regulating the expression levels and patterns of
CC the transcription factor PLETHORA (PLT), mainly at the post-
CC transcriptional level (PubMed:20798316, PubMed:23370719). Maintains the
CC postembryonic root stem cell niche by regulating the expression levels
CC and patterns of the transcription factor PLETHORA (e.g. PLT1 and PLT2),
CC mainly at the post-transcriptional level (PubMed:20798316). Influences
CC circumferential cell number in the root meristem in response to Pi-
CC deprivation (PubMed:25856240). Regulates the pattern of root growth and
CC lateral root development by modulating the length and the number of
CC cortical cells in the root apical meristem (RAM), and the anticlinal
CC asymmetric cell divisions in lateral root initiation cells
CC (PubMed:22307643, PubMed:23370719). {ECO:0000269|PubMed:20798316,
CC ECO:0000269|PubMed:22307643, ECO:0000269|PubMed:23370719,
CC ECO:0000269|PubMed:25856240, ECO:0000269|PubMed:31801996}.
CC -!- SUBUNIT: [GLV11p]: Interacts with RGI1; this interaction triggers RGI1
CC phosphorylation and ubiquitination (PubMed:27229312). Binds to LRR
CC receptor-like serine/threonine-protein kinases RGI1, RGI2 and RGI3 to
CC trigger their dimerization with SERK proteins and subsequent signaling
CC (PubMed:27229311, PubMed:27001831). {ECO:0000269|PubMed:27001831,
CC ECO:0000269|PubMed:27229311, ECO:0000269|PubMed:27229312}.
CC -!- SUBCELLULAR LOCATION: [GLV11p]: Secreted {ECO:0000269|PubMed:20798316}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3E880-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3E880-2; Sequence=VSP_040182;
CC -!- TISSUE SPECIFICITY: Expressed in root tips, only in the quiescent
CC center and the columella stem cells. {ECO:0000269|PubMed:20798316,
CC ECO:0000269|PubMed:23370719}.
CC -!- DEVELOPMENTAL STAGE: In roots, expressed only in the quiescent center
CC (QC) and the columella stem cells (CC) initials (PubMed:20798316,
CC PubMed:23370719). Induced during lateral root formation
CC (PubMed:23370719). {ECO:0000269|PubMed:20798316,
CC ECO:0000269|PubMed:23370719}.
CC -!- INDUCTION: Not regulated by auxin (PubMed:20798316). Accumulates in
CC both root epidermis and cortex after phosphate ion (Pi)-deprivation
CC (PubMed:25856240). {ECO:0000269|PubMed:20798316,
CC ECO:0000269|PubMed:25856240}.
CC -!- PTM: The tyrosine sulfation is critical for the function of the
CC peptide. {ECO:0000269|PubMed:20798316}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, due to the redundancy with
CC other RGF genes (PubMed:20798316). Hypersensitivity to low phosphate
CC ion (Pi) with respect to circumferential cortex and epidermis cell
CC quantities (PubMed:25856240). Triple mutant rgf1-rgf2-rgf3 shows a
CC decreased meristematic cell number resulting in a short root phenotype
CC (PubMed:20798316). {ECO:0000269|PubMed:20798316,
CC ECO:0000269|PubMed:25856240}.
CC -!- MISCELLANEOUS: Diffuses into the meristematic region.
CC {ECO:0000305|PubMed:20798316}.
CC -!- MISCELLANEOUS: 'Golven' means irregular waves in Dutch.
CC {ECO:0000305|PubMed:23370719}.
CC -!- SIMILARITY: Belongs to the RGF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28769.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB568259; BAJ41538.1; -; mRNA.
DR EMBL; AB015472; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED97381.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97382.1; -; Genomic_DNA.
DR EMBL; DQ447098; ABE66266.1; -; mRNA.
DR EMBL; DQ653385; ABK28769.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001119468.1; NM_001125996.2. [Q3E880-2]
DR RefSeq; NP_200889.2; NM_125474.4. [Q3E880-1]
DR PDB; 5HYX; X-ray; 2.60 A; A=104-116.
DR PDBsum; 5HYX; -.
DR AlphaFoldDB; Q3E880; -.
DR SMR; Q3E880; -.
DR STRING; 3702.AT5G60810.1; -.
DR PaxDb; Q3E880; -.
DR PRIDE; Q3E880; -.
DR EnsemblPlants; AT5G60810.1; AT5G60810.1; AT5G60810. [Q3E880-1]
DR EnsemblPlants; AT5G60810.2; AT5G60810.2; AT5G60810. [Q3E880-2]
DR GeneID; 836202; -.
DR Gramene; AT5G60810.1; AT5G60810.1; AT5G60810. [Q3E880-1]
DR Gramene; AT5G60810.2; AT5G60810.2; AT5G60810. [Q3E880-2]
DR KEGG; ath:AT5G60810; -.
DR Araport; AT5G60810; -.
DR TAIR; locus:2159315; AT5G60810.
DR eggNOG; ENOG502R1V1; Eukaryota.
DR HOGENOM; CLU_2100270_0_0_1; -.
DR OMA; IRVICYL; -.
DR OrthoDB; 1550538at2759; -.
DR PRO; PR:Q3E880; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q3E880; baseline and differential.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0008083; F:growth factor activity; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IDA:UniProtKB.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IMP:UniProtKB.
DR GO; GO:0010078; P:maintenance of root meristem identity; IMP:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IEP:TAIR.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IDA:UniProtKB.
DR GO; GO:0009786; P:regulation of asymmetric cell division; ISS:UniProtKB.
DR GO; GO:0051302; P:regulation of cell division; IMP:UniProtKB.
DR GO; GO:2000023; P:regulation of lateral root development; IDA:UniProtKB.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; IMP:UniProtKB.
DR GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR GO; GO:0010082; P:regulation of root meristem growth; IGI:TAIR.
DR GO; GO:2000067; P:regulation of root morphogenesis; IDA:UniProtKB.
DR InterPro; IPR038804; RGF1/2/3.
DR PANTHER; PTHR36313; PTHR36313; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Differentiation;
KW Direct protein sequencing; Glycoprotein; Growth factor; Hydroxylation;
KW Reference proteome; Secreted; Signal; Sulfation.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT PROPEP 21..103
FT /evidence="ECO:0000269|PubMed:20798316"
FT /id="PRO_0000401438"
FT PEPTIDE 104..116
FT /note="GLV11p"
FT /id="PRO_0000401439"
FT REGION 82..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 105
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000269|PubMed:20798316"
FT MOD_RES 113
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CARBOHYD 44
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 1..30
FT /note="MVSIRVICYLLVFSVLQVHAKVSNANFNSQ -> MYVNKFYHAYQNFYI
FT (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040182"
SQ SEQUENCE 116 AA; 13029 MW; E8BE4DBCD8785EF6 CRC64;
MVSIRVICYL LVFSVLQVHA KVSNANFNSQ APQMKNSEGL GASNGTQIAK KHAEDVIENR
KTLKHVNVKV EANEKNGLEI ESKEMVKKRK NKKRLTKTES LTADYSNPGH HPPRHN
