ID   GLV2_ARATH              Reviewed;          79 AA.
AC   Q9FGF6;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Protein GOLVEN 2 {ECO:0000303|PubMed:23370719};
DE   AltName: Full=CLAVATA3/ESR (CLE)-related protein CLEL9 {ECO:0000303|PubMed:22307643};
DE            Short=CLE-Like protein 9 {ECO:0000303|PubMed:22307643};
DE   AltName: Full=Root meristem growth factor 9 {ECO:0000303|PubMed:20798316};
DE            Short=AtRGF9 {ECO:0000303|PubMed:20798316};
DE   Contains:
DE     RecName: Full=GLV2p {ECO:0000303|PubMed:22421050};
DE   Flags: Precursor;
GN   Name=GLV2 {ECO:0000303|PubMed:23370719};
GN   Synonyms=CLEL9 {ECO:0000303|PubMed:22307643},
GN   RGF9 {ECO:0000303|PubMed:20798316};
GN   OrderedLocusNames=At5g64770 {ECO:0000312|Araport:AT5G64770};
GN   ORFNames=MVP7.10 {ECO:0000312|EMBL:BAB10310.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=20798316; DOI=10.1126/science.1191132;
RA   Matsuzaki Y., Ogawa-Ohnishi M., Mori A., Matsubayashi Y.;
RT   "Secreted peptide signals required for maintenance of root stem cell niche
RT   in Arabidopsis.";
RL   Science 329:1065-1067(2010).
RN   [5]
RP   FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   IDENTIFICATION BY MASS SPECTROMETRY, HYDROXYLATION AT PRO-75, SULFATION AT
RP   TYR-67, AND INDUCTION BY AUXIN.
RX   PubMed=22421050; DOI=10.1016/j.devcel.2012.02.002;
RA   Whitford R., Fernandez A., Tejos R., Perez A.C., Kleine-Vehn J.,
RA   Vanneste S., Drozdzecki A., Leitner J., Abas L., Aerts M., Hoogewijs K.,
RA   Baster P., De Groodt R., Lin Y.-C., Storme V., Van de Peer Y., Beeckman T.,
RA   Madder A., Devreese B., Luschnig C., Friml J., Hilson P.;
RT   "GOLVEN secretory peptides regulate auxin carrier turnover during plant
RT   gravitropic responses.";
RL   Dev. Cell 22:678-685(2012).
RN   [6]
RP   FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, NOMENCLATURE, AND GENE
RP   FAMILY.
RC   STRAIN=cv. Columbia;
RX   PubMed=22307643; DOI=10.1073/pnas.1119864109;
RA   Meng L., Buchanan B.B., Feldman L.J., Luan S.;
RT   "CLE-like (CLEL) peptides control the pattern of root growth and lateral
RT   root development in Arabidopsis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:1760-1765(2012).
RN   [7]
RP   FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Columbia;
RX   PubMed=23370719; DOI=10.1104/pp.112.206029;
RA   Fernandez A., Drozdzecki A., Hoogewijs K., Nguyen A., Beeckman T.,
RA   Madder A., Hilson P.;
RT   "Transcriptional and functional classification of the GOLVEN/ROOT GROWTH
RT   FACTOR/CLE-like signaling peptides reveals their role in lateral root and
RT   hair formation.";
RL   Plant Physiol. 161:954-970(2013).
CC   -!- FUNCTION: [GLV2p]: Signaling peptide (root growth factor) that
CC       regulates the pattern of root growth and lateral root development by
CC       modulating the length and the number of cortical cells in the root
CC       apical meristem (RAM), and the anticlinal asymmetric cell divisions in
CC       lateral root initiation cells (PubMed:22307643, PubMed:23370719). Also
CC       involved in the regulation of hypocotyl bending and root gravitropism,
CC       probably by influencing the formation of auxin gradients
CC       (PubMed:22421050). Maintains the postembryonic root stem cell niche
CC       (PubMed:20798316). {ECO:0000269|PubMed:20798316,
CC       ECO:0000269|PubMed:22307643, ECO:0000269|PubMed:22421050,
CC       ECO:0000269|PubMed:23370719}.
CC   -!- SUBUNIT: [GLV2p]: Binds to LRR receptor-like serine/threonine-protein
CC       kinases to trigger their dimerization with SERK proteins and subsequent
CC       signaling. {ECO:0000250|UniProtKB:Q9LI64}.
CC   -!- SUBCELLULAR LOCATION: [GLV2p]: Secreted {ECO:0000305|PubMed:22307643}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC       {ECO:0000269|PubMed:22307643}. Note=The precursor is detected in the
CC       endoplasmic reticulum probably during its processing.
CC       {ECO:0000269|PubMed:22307643}.
CC   -!- TISSUE SPECIFICITY: Expressed in siliques, stems, hypocotyls, shoot
CC       apex, leaves, flowers and cotyledons, and, to a lower extent, in roots.
CC       {ECO:0000269|PubMed:22307643, ECO:0000269|PubMed:22421050,
CC       ECO:0000269|PubMed:23370719}.
CC   -!- DEVELOPMENTAL STAGE: Absent from the primary root (PubMed:23370719).
CC       Induced during lateral root formation after the emergence of the
CC       primordium and in mature lateral roots (PubMed:23370719). Expressed
CC       homogeneously across the cotyledon (PubMed:23370719). In leaves,
CC       expressed in the whole lamina, with a stronger signal in the outer part
CC       of the leaf (PubMed:23370719). Higher levels in younger leaves and
CC       fades out as leaves expand (PubMed:23370719). Observed in the stem and
CC       sepals, and in the gynoecium of the developing flowers, and later in
CC       siliques (PubMed:23370719). During seedling gravitropic response,
CC       restricted to the epidermis and cortex and enhanced at the lower side
CC       of the reoriented hypocotyl (PubMed:22421050).
CC       {ECO:0000269|PubMed:22421050, ECO:0000269|PubMed:23370719}.
CC   -!- INDUCTION: Rapidly induced by auxin. {ECO:0000269|PubMed:22421050}.
CC   -!- DISRUPTION PHENOTYPE: Reduced hypocotyl bending and dose-dependent
CC       altered root gravitropism with an impaired formation of auxin
CC       gradients, thus leading to an irregular waves root shape.
CC       {ECO:0000269|PubMed:22421050}.
CC   -!- MISCELLANEOUS: 'Golven' means irregular waves in Dutch.
CC       {ECO:0000305|PubMed:22421050}.
CC   -!- SIMILARITY: Belongs to the RGF family. {ECO:0000305}.
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DR   EMBL; AB025637; BAB10310.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97949.1; -; Genomic_DNA.
DR   EMBL; BT002516; AAO00876.1; -; mRNA.
DR   EMBL; BT006500; AAP21308.1; -; mRNA.
DR   RefSeq; NP_201282.1; NM_125873.3.
DR   AlphaFoldDB; Q9FGF6; -.
DR   STRING; 3702.AT5G64770.1; -.
DR   PaxDb; Q9FGF6; -.
DR   PRIDE; Q9FGF6; -.
DR   EnsemblPlants; AT5G64770.1; AT5G64770.1; AT5G64770.
DR   GeneID; 836598; -.
DR   Gramene; AT5G64770.1; AT5G64770.1; AT5G64770.
DR   KEGG; ath:AT5G64770; -.
DR   Araport; AT5G64770; -.
DR   TAIR; locus:2176060; AT5G64770.
DR   HOGENOM; CLU_174364_0_0_1; -.
DR   InParanoid; Q9FGF6; -.
DR   OMA; RNIGGIM; -.
DR   OrthoDB; 1580392at2759; -.
DR   PhylomeDB; Q9FGF6; -.
DR   PRO; PR:Q9FGF6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FGF6; baseline and differential.
DR   Genevisible; Q9FGF6; AT.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR   GO; GO:0008083; F:growth factor activity; IDA:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IDA:UniProtKB.
DR   GO; GO:0030154; P:cell differentiation; IDA:UniProtKB.
DR   GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR   GO; GO:0009958; P:positive gravitropism; IMP:TAIR.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:UniProtKB.
DR   GO; GO:0009786; P:regulation of asymmetric cell division; ISS:UniProtKB.
DR   GO; GO:2000012; P:regulation of auxin polar transport; IMP:TAIR.
DR   GO; GO:2000023; P:regulation of lateral root development; IDA:UniProtKB.
DR   GO; GO:0032880; P:regulation of protein localization; IDA:TAIR.
DR   GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR   GO; GO:0010082; P:regulation of root meristem growth; IDA:UniProtKB.
DR   GO; GO:2000067; P:regulation of root morphogenesis; IDA:UniProtKB.
DR   GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
DR   GO; GO:0022622; P:root system development; IMP:TAIR.
DR   InterPro; IPR039337; GLV2.
DR   PANTHER; PTHR33743; PTHR33743; 1.
PE   1: Evidence at protein level;
KW   Differentiation; Endoplasmic reticulum; Growth factor; Hydroxylation;
KW   Reference proteome; Secreted; Signal; Sulfation.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   PROPEP          27..65
FT                   /evidence="ECO:0000250|UniProtKB:Q3E880"
FT                   /id="PRO_0000401457"
FT   PEPTIDE         66..78
FT                   /note="GLV2p"
FT                   /id="PRO_0000401458"
FT   REGION          49..79
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         67
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000269|PubMed:22421050"
FT   MOD_RES         75
FT                   /note="Hydroxyproline"
FT                   /evidence="ECO:0000269|PubMed:22421050"
SQ   SEQUENCE   79 AA;  8987 MW;  2A8F5114F52794AE CRC64;
     MAIRVSHKSF LVALLLILFI SSPTQARSLR EVVRNRTLLV VEKSQESRKI RHEGGGSDVD
     GLMDMDYNSA NKKRPIHNR