GLV3_ARATH
ID GLV3_ARATH Reviewed; 163 AA.
AC Q9LI64; Q8L8Q0;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Protein GOLVEN 3 {ECO:0000303|PubMed:23370719};
DE AltName: Full=Root meristem growth factor 4 {ECO:0000303|PubMed:20798316};
DE Short=AtRGF4 {ECO:0000303|PubMed:20798316};
DE Contains:
DE RecName: Full=GLV3p {ECO:0000303|PubMed:22421050};
DE Flags: Precursor;
GN Name=GLV3 {ECO:0000303|PubMed:23370719};
GN Synonyms=RGF4 {ECO:0000303|PubMed:20798316};
GN OrderedLocusNames=At3g30350 {ECO:0000312|Araport:AT3G30350};
GN ORFNames=T6J22.14 {ECO:0000312|EMBL:BAB02236.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20798316; DOI=10.1126/science.1191132;
RA Matsuzaki Y., Ogawa-Ohnishi M., Mori A., Matsubayashi Y.;
RT "Secreted peptide signals required for maintenance of root stem cell niche
RT in Arabidopsis.";
RL Science 329:1065-1067(2010).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP IDENTIFICATION BY MASS SPECTROMETRY, HYDROXYLATION AT PRO-154, AND
RP SULFATION AT TYR-143.
RX PubMed=22421050; DOI=10.1016/j.devcel.2012.02.002;
RA Whitford R., Fernandez A., Tejos R., Perez A.C., Kleine-Vehn J.,
RA Vanneste S., Drozdzecki A., Leitner J., Abas L., Aerts M., Hoogewijs K.,
RA Baster P., De Groodt R., Lin Y.-C., Storme V., Van de Peer Y., Beeckman T.,
RA Madder A., Devreese B., Luschnig C., Friml J., Hilson P.;
RT "GOLVEN secretory peptides regulate auxin carrier turnover during plant
RT gravitropic responses.";
RL Dev. Cell 22:678-685(2012).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=23370719; DOI=10.1104/pp.112.206029;
RA Fernandez A., Drozdzecki A., Hoogewijs K., Nguyen A., Beeckman T.,
RA Madder A., Hilson P.;
RT "Transcriptional and functional classification of the GOLVEN/ROOT GROWTH
RT FACTOR/CLE-like signaling peptides reveals their role in lateral root and
RT hair formation.";
RL Plant Physiol. 161:954-970(2013).
RN [7]
RP INTERACTION WITH RGI1; RGI2 AND RGI3.
RC STRAIN=cv. Columbia;
RX PubMed=27001831; DOI=10.1073/pnas.1522639113;
RA Shinohara H., Mori A., Yasue N., Sumida K., Matsubayashi Y.;
RT "Identification of three LRR-RKs involved in perception of root meristem
RT growth factor in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:3897-3902(2016).
CC -!- FUNCTION: [GLV3p]: Signaling peptide (root growth factor) required
CC during root gravitropism in a PIN2-traffic dependent manner, thus
CC influencing the formation of auxin gradients (PubMed:22421050,
CC PubMed:23370719). Maintains the postembryonic root stem cell niche
CC (PubMed:20798316). {ECO:0000269|PubMed:20798316,
CC ECO:0000269|PubMed:22421050, ECO:0000269|PubMed:23370719}.
CC -!- SUBUNIT: [GLV3p]: Binds to LRR receptor-like serine/threonine-protein
CC kinases RGI1, RGI2 and RGI3 to trigger their dimerization with SERK
CC proteins and subsequent signaling. {ECO:0000269|PubMed:27001831}.
CC -!- SUBCELLULAR LOCATION: [GLV3p]: Secreted {ECO:0000250|UniProtKB:Q3E880}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LI64-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in roots, specifically in the root apical
CC meristem (RAM). {ECO:0000269|PubMed:22421050,
CC ECO:0000269|PubMed:23370719}.
CC -!- DEVELOPMENTAL STAGE: In roots, restricted to the root apical meristem
CC (RAM) in a pattern positioned just above the quiescent center (QC),
CC mainly in endodermis, cortex and vascular tissues, with strongest
CC levels within cells in the QC vicinity (PubMed:23370719,
CC PubMed:22421050). Slightly observed in the epidermis (PubMed:23370719).
CC Induced early during lateral root formation (PubMed:23370719).
CC {ECO:0000269|PubMed:22421050, ECO:0000269|PubMed:23370719}.
CC -!- DISRUPTION PHENOTYPE: Dose-dependent altered root gravitropism
CC associated with an altered PIN2 traffic that impairs the formation of
CC auxin gradients, thus leading to an irregular waves root shape.
CC {ECO:0000269|PubMed:22421050, ECO:0000269|PubMed:23370719}.
CC -!- MISCELLANEOUS: 'Golven' means irregular waves in Dutch.
CC {ECO:0000305|PubMed:22421050}.
CC -!- SIMILARITY: Belongs to the RGF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02236.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP001314; BAB02236.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77634.1; -; Genomic_DNA.
DR EMBL; AY088881; AAM67187.1; -; mRNA.
DR RefSeq; NP_001319668.1; NM_001339050.1.
DR RefSeq; NP_566853.1; NM_113934.3. [Q9LI64-1]
DR AlphaFoldDB; Q9LI64; -.
DR PRIDE; Q9LI64; -.
DR EnsemblPlants; AT3G30350.1; AT3G30350.1; AT3G30350. [Q9LI64-1]
DR GeneID; 822736; -.
DR Gramene; AT3G30350.1; AT3G30350.1; AT3G30350. [Q9LI64-1]
DR KEGG; ath:AT3G30350; -.
DR Araport; AT3G30350; -.
DR HOGENOM; CLU_1629335_0_0_1; -.
DR InParanoid; Q9LI64; -.
DR OMA; HNRAPDK; -.
DR OrthoDB; 1472046at2759; -.
DR PRO; PR:Q9LI64; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LI64; baseline and differential.
DR Genevisible; Q9LI64; AT.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0008083; F:growth factor activity; IDA:UniProtKB.
DR GO; GO:0005179; F:hormone activity; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IDA:UniProtKB.
DR GO; GO:0009958; P:positive gravitropism; IMP:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:UniProtKB.
DR GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Differentiation; Growth factor; Hydroxylation;
KW Reference proteome; Secreted; Signal; Sulfation.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT PROPEP 21..141
FT /evidence="ECO:0000250|UniProtKB:Q3E880"
FT /id="PRO_0000401444"
FT PEPTIDE 142..157
FT /note="GLV3p"
FT /id="PRO_0000401445"
FT PROPEP 158..163
FT /evidence="ECO:0000250|UniProtKB:Q3E880"
FT /id="PRO_0000401446"
FT REGION 144..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 143
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000269|PubMed:22421050"
FT MOD_RES 154
FT /note="Hydroxyproline"
FT /evidence="ECO:0000269|PubMed:22421050"
FT CONFLICT 22
FT /note="H -> Q (in Ref. 3; AAM67187)"
FT /evidence="ECO:0000305"
FT CONFLICT 28..34
FT /note="HEGGEAG -> RKGREAC (in Ref. 3; AAM67187)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="F -> Y (in Ref. 3; AAM67187)"
FT /evidence="ECO:0000305"
FT CONFLICT 66
FT /note="T -> A (in Ref. 3; AAM67187)"
FT /evidence="ECO:0000305"
FT CONFLICT 146
FT /note="Y -> H (in Ref. 3; AAM67187)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 163 AA; 18735 MW; A5B7073C47F08AF6 CRC64;
MMRFTIIVIA FLLIIQSLEE EHILVYAHEG GEAGHKSLDY QGDQDSSTLH PKELFDAPRK
VRFGRTTRAE KEQVTAMNND SWSFKISGEH KQTNILADHD TTKNTFCKKM MIIVNDLTSL
PTLEPSTSTN DMEKLARLLR DDYPIYSKPR RKPPVNNRAP DKF
