GLV5_ARATH
ID GLV5_ARATH Reviewed; 109 AA.
AC Q6DSU1; Q4PT32; Q9LMY6;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Protein GOLVEN 5 {ECO:0000303|PubMed:23370719};
DE AltName: Full=CLAVATA3/ESR (CLE)-related protein CLEL1 {ECO:0000303|PubMed:22307643};
DE Short=CLE-Like protein 1 {ECO:0000303|PubMed:22307643};
DE AltName: Full=Root meristem growth factor 2 {ECO:0000303|PubMed:20798316};
DE Short=AtRGF2 {ECO:0000303|PubMed:20798316};
DE Contains:
DE RecName: Full=GLV5p {ECO:0000303|PubMed:23370719};
DE Flags: Precursor;
GN Name=GLV5 {ECO:0000303|PubMed:23370719};
GN Synonyms=CLEL1 {ECO:0000303|PubMed:22307643},
GN RGF2 {ECO:0000303|PubMed:20798316};
GN OrderedLocusNames=At1g13620 {ECO:0000312|Araport:AT1G13620};
GN ORFNames=F21F23.5 {ECO:0000312|EMBL:AAF81288.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20798316; DOI=10.1126/science.1191132;
RA Matsuzaki Y., Ogawa-Ohnishi M., Mori A., Matsubayashi Y.;
RT "Secreted peptide signals required for maintenance of root stem cell niche
RT in Arabidopsis.";
RL Science 329:1065-1067(2010).
RN [5]
RP NOMENCLATURE, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=22307643; DOI=10.1073/pnas.1119864109;
RA Meng L., Buchanan B.B., Feldman L.J., Luan S.;
RT "CLE-like (CLEL) peptides control the pattern of root growth and lateral
RT root development in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:1760-1765(2012).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=23370719; DOI=10.1104/pp.112.206029;
RA Fernandez A., Drozdzecki A., Hoogewijs K., Nguyen A., Beeckman T.,
RA Madder A., Hilson P.;
RT "Transcriptional and functional classification of the GOLVEN/ROOT GROWTH
RT FACTOR/CLE-like signaling peptides reveals their role in lateral root and
RT hair formation.";
RL Plant Physiol. 161:954-970(2013).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY PHOSPHATE-DEPRIVATION.
RC STRAIN=cv. Columbia;
RX PubMed=25856240; DOI=10.1111/nph.13405;
RA Cederholm H.M., Benfey P.N.;
RT "Distinct sensitivities to phosphate deprivation suggest that RGF peptides
RT play disparate roles in Arabidopsis thaliana root development.";
RL New Phytol. 207:683-691(2015).
RN [8]
RP INTERACTION WITH RGI1; RGI2 AND RGI3.
RC STRAIN=cv. Columbia;
RX PubMed=27001831; DOI=10.1073/pnas.1522639113;
RA Shinohara H., Mori A., Yasue N., Sumida K., Matsubayashi Y.;
RT "Identification of three LRR-RKs involved in perception of root meristem
RT growth factor in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:3897-3902(2016).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS) OF 97-109.
RA Song W., Han Z., Chai J.;
RT "Plant Receptor.";
RL Submitted (FEB-2016) to the PDB data bank.
CC -!- FUNCTION: [GLV5p]: Signaling peptide (root growth factor) that
CC maintains the postembryonic root stem cell niche in a PIN2-traffic
CC dependent manner (PubMed:20798316, PubMed:23370719). Root growth factor
CC that regulates the pattern of root growth and lateral root development
CC by modulating the length and the number of cortical cells in the root
CC apical meristem (RAM), and the anticlinal asymmetric cell divisions in
CC lateral root initiation cells (By similarity). Influences the
CC longitudinal growth rate in the primary root in response to phosphate
CC ion (Pi)-deprivation (PubMed:25856240). {ECO:0000250|UniProtKB:Q93VK8,
CC ECO:0000269|PubMed:20798316, ECO:0000269|PubMed:23370719,
CC ECO:0000269|PubMed:25856240}.
CC -!- SUBUNIT: [GLV5p]: Binds to LRR receptor-like serine/threonine-protein
CC kinases RGI1, RGI2 and RGI3 to trigger their dimerization with SERK
CC proteins and subsequent signaling. {ECO:0000269|PubMed:27001831}.
CC -!- SUBCELLULAR LOCATION: [GLV5p]: Secreted {ECO:0000250|UniProtKB:Q3E880}.
CC -!- TISSUE SPECIFICITY: Expressed in root tips.
CC {ECO:0000269|PubMed:20798316, ECO:0000269|PubMed:23370719}.
CC -!- DEVELOPMENTAL STAGE: In roots, expressed only in the quiescent center
CC (QC), the columella stem cells (CC) and the innermost layer of central
CC columella cells; mostly present in the second, third and fourth CC
CC layers, at a lower level in undifferentiated CCs, but not, or only
CC marginally, in the QC (PubMed:20798316, PubMed:23370719). Induced early
CC during lateral root formation (PubMed:23370719).
CC {ECO:0000269|PubMed:20798316, ECO:0000269|PubMed:23370719}.
CC -!- INDUCTION: Accumulates in both root epidermis and cortex after
CC Pi- deprivation. {ECO:0000269|PubMed:25856240}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, due to the redundancy with
CC other RGF genes (PubMed:20798316, PubMed:23370719). Slower root growth
CC after (Pi)-deprivation (PubMed:25856240). Triple mutant rgf1-rgf2-rgf3
CC shows a decreased meristematic cell number resulting in a short root
CC phenotype associated with an altered PIN2 traffic (PubMed:20798316,
CC PubMed:23370719). {ECO:0000269|PubMed:20798316,
CC ECO:0000269|PubMed:23370719, ECO:0000269|PubMed:25856240}.
CC -!- MISCELLANEOUS: 'Golven' means irregular waves in Dutch.
CC {ECO:0000305|PubMed:23370719}.
CC -!- SIMILARITY: Belongs to the RGF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF81288.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC027656; AAF81288.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29048.1; -; Genomic_DNA.
DR EMBL; AY648307; AAT68718.1; -; mRNA.
DR EMBL; DQ056454; AAY78611.1; -; mRNA.
DR PIR; C86269; C86269.
DR RefSeq; NP_172819.2; NM_101232.3.
DR PDB; 5HZ0; X-ray; 2.56 A; A=97-109.
DR PDBsum; 5HZ0; -.
DR AlphaFoldDB; Q6DSU1; -.
DR SMR; Q6DSU1; -.
DR STRING; 3702.AT1G13620.1; -.
DR PaxDb; Q6DSU1; -.
DR PRIDE; Q6DSU1; -.
DR EnsemblPlants; AT1G13620.1; AT1G13620.1; AT1G13620.
DR GeneID; 837923; -.
DR Gramene; AT1G13620.1; AT1G13620.1; AT1G13620.
DR KEGG; ath:AT1G13620; -.
DR Araport; AT1G13620; -.
DR TAIR; locus:2009982; AT1G13620.
DR HOGENOM; CLU_175812_0_0_1; -.
DR InParanoid; Q6DSU1; -.
DR OMA; TTKMKIN; -.
DR OrthoDB; 1614476at2759; -.
DR PhylomeDB; Q6DSU1; -.
DR PRO; PR:Q6DSU1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6DSU1; baseline and differential.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0008083; F:growth factor activity; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IDA:UniProtKB.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IMP:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IBA:GO_Central.
DR GO; GO:0009786; P:regulation of asymmetric cell division; ISS:UniProtKB.
DR GO; GO:2000023; P:regulation of lateral root development; ISS:UniProtKB.
DR GO; GO:2000280; P:regulation of root development; IMP:UniProtKB.
DR GO; GO:0010082; P:regulation of root meristem growth; IGI:TAIR.
DR GO; GO:2000067; P:regulation of root morphogenesis; IMP:UniProtKB.
DR InterPro; IPR038804; RGF1/2/3.
DR PANTHER; PTHR36313; PTHR36313; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Differentiation; Growth factor; Hydroxylation;
KW Reference proteome; Secreted; Signal; Sulfation.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..96
FT /evidence="ECO:0000250|UniProtKB:Q3E880"
FT /id="PRO_0000401440"
FT PEPTIDE 97..109
FT /note="GLV5p"
FT /id="PRO_0000401441"
FT REGION 54..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 98
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q3E880"
FT MOD_RES 106
FT /note="Hydroxyproline"
FT /evidence="ECO:0000250|UniProtKB:O49519"
FT CONFLICT 4..6
FT /note="ITS -> VTA (in Ref. 3; AAY78611)"
FT /evidence="ECO:0000305"
FT CONFLICT 109
FT /note="N -> T (in Ref. 3; AAY78611)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 109 AA; 12385 MW; 98AAE820463D7096 CRC64;
MTNITSSFLC LLILLLFCLS FGYSLHGDKD EVLSVDVGSN AKVMKHLDGD DAMKKAQVRG
RSGQEFSKET TKMMMKKTTK KETNVEEEDD LVAYTADYWK PRHHPPKNN
