ID   AMIF_PSESM              Reviewed;         338 AA.
AC   Q887D9;
DT   30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2003, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Formamidase {ECO:0000255|HAMAP-Rule:MF_01243};
DE            EC=3.5.1.49 {ECO:0000255|HAMAP-Rule:MF_01243};
DE   AltName: Full=Formamide amidohydrolase {ECO:0000255|HAMAP-Rule:MF_01243};
GN   Name=amiF {ECO:0000255|HAMAP-Rule:MF_01243}; Synonyms=amiE;
GN   OrderedLocusNames=PSPTO_1357;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: Is an aliphatic amidase with a restricted substrate
CC       specificity, as it only hydrolyzes formamide. {ECO:0000255|HAMAP-
CC       Rule:MF_01243}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=formamide + H2O = formate + NH4(+); Xref=Rhea:RHEA:21948,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15740, ChEBI:CHEBI:16397,
CC         ChEBI:CHEBI:28938; EC=3.5.1.49; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01243};
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       Aliphatic amidase family. {ECO:0000255|HAMAP-Rule:MF_01243}.
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DR   EMBL; AE016853; AAO54879.1; -; Genomic_DNA.
DR   RefSeq; NP_791184.1; NC_004578.1.
DR   RefSeq; WP_011103524.1; NC_004578.1.
DR   AlphaFoldDB; Q887D9; -.
DR   SMR; Q887D9; -.
DR   STRING; 223283.PSPTO_1357; -.
DR   EnsemblBacteria; AAO54879; AAO54879; PSPTO_1357.
DR   GeneID; 1182993; -.
DR   KEGG; pst:PSPTO_1357; -.
DR   PATRIC; fig|223283.9.peg.1379; -.
DR   eggNOG; COG0388; Bacteria.
DR   HOGENOM; CLU_071797_0_0_6; -.
DR   OMA; RIWGCFS; -.
DR   OrthoDB; 1650683at2; -.
DR   PhylomeDB; Q887D9; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0004040; F:amidase activity; ISS:JCVI.
DR   GO; GO:0004328; F:formamidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.60.110.10; -; 1.
DR   HAMAP; MF_01243; Formamidase; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR022843; Formamidase.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..338
FT                   /note="Formamidase"
FT                   /id="PRO_0000204067"
FT   DOMAIN          15..257
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        61
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01243"
FT   ACT_SITE        130
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01243"
FT   ACT_SITE        163
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01243"
SQ   SEQUENCE   338 AA;  37253 MW;  1B47EF8A07392DD1 CRC64;
     MASGLGGLNK SPNGVVIGLA QLALPDPHTR EALWAQTQKV VGMVAKARRS NPGMDLIVFP
     EYSLHGLSMS TAPEIMCSLD GPEVVALREA CKEHRIWGCF SIMEANPQGN PFNSSLIVDD
     LGEIRLYYRK LHPWVPVEPW EPGDLGIPVC DGPRGSTLAL IICHDGMFPE MARECAYKGA
     DIMLRTAGYT APIRHSWKIT NQSNAFTNLM QTASVCMCGS DGTFDSMGEA MFVDFDGTIM
     AEGGGRADEI VCCELRPDLV REARVHWGVE NNIYQFGHRG YVAVKGGARD CPYTYMRDLT
     AGQYRLPWEN DVVHTDGRSC GFATPEREFK PTPSSWKE