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GLYAT_HUMAN
ID   GLYAT_HUMAN             Reviewed;         296 AA.
AC   Q6IB77; H1AE11; O14833; Q96QK7;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 3.
DT   03-AUG-2022, entry version 141.
DE   RecName: Full=Glycine N-acyltransferase {ECO:0000303|PubMed:10630424};
DE            EC=2.3.1.13 {ECO:0000303|PubMed:22475485, ECO:0000303|PubMed:7802672};
DE   AltName: Full=Acyl-CoA:glycine N-acyltransferase;
DE            Short=AAc;
DE   AltName: Full=Aralkyl acyl-CoA N-acyltransferase;
DE   AltName: Full=Aralkyl acyl-CoA:amino acid N-acyltransferase;
DE   AltName: Full=Benzoyl-coenzyme A:glycine N-acyltransferase;
DE   AltName: Full=Glycine N-benzoyltransferase;
DE            EC=2.3.1.71 {ECO:0000303|PubMed:22475485, ECO:0000303|PubMed:7802672};
DE   AltName: Full=HRP-1(CLP);
GN   Name=GLYAT; Synonyms=ACGNAT, CAT, GAT;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND VARIANT SER-156.
RX   PubMed=10630424;
RX   DOI=10.1002/(sici)1099-0461(2000)14:2<102::aid-jbt6>3.0.co;2-h;
RA   van der Westhuizen F.H., Pretorius P.J., Erasmus E.;
RT   "The utilization of alanine, glutamic acid, and serine as amino acid
RT   substrates for glycine N-acyltransferase.";
RL   J. Biochem. Mol. Toxicol. 14:102-109(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND VARIANT SER-156.
RX   PubMed=22475485; DOI=10.1016/j.bbrc.2012.03.099;
RA   Matsuo M., Terai K., Kameda N., Matsumoto A., Kurokawa Y., Funase Y.,
RA   Nishikawa K., Sugaya N., Hiruta N., Kishimoto T.;
RT   "Designation of enzyme activity of glycine-N-acyltransferase family genes
RT   and depression of glycine-N-acyltransferase in human hepatocellular
RT   carcinoma.";
RL   Biochem. Biophys. Res. Commun. 420:901-906(2012).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND VARIANT SER-156.
RA   Kishimoto T., Niwa S., Nishikawa K.;
RT   "Human HRP-1(CLP).";
RL   Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16554811; DOI=10.1038/nature04632;
RA   Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA   Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA   Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA   Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA   Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA   Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT   "Human chromosome 11 DNA sequence and analysis including novel gene
RT   identification.";
RL   Nature 440:497-500(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANTS THR-17 AND
RP   SER-156.
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-163, AND VARIANT SER-156.
RA   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   CATALYTIC ACTIVITY, FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=7802672; DOI=10.1006/bbrc.1994.2817;
RA   Mawal Y.R., Qureshi I.A.;
RT   "Purification to homogeneity of mitochondrial acyl CoA:glycine N-
RT   acyltransferase from human liver.";
RL   Biochem. Biophys. Res. Commun. 205:1373-1379(1994).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- FUNCTION: Mitochondrial acyltransferase which transfers an acyl group
CC       to the N-terminus of glycine and glutamine, although much less
CC       efficiently. Can conjugate numerous substrates to form a variety of N-
CC       acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA
CC       as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or
CC       salicylic acid, and endogenous organic acids, such as isovaleric acid.
CC       {ECO:0000269|PubMed:22475485, ECO:0000269|PubMed:7802672}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an acyl-CoA + glycine = an N-acylglycine + CoA + H(+);
CC         Xref=Rhea:RHEA:19869, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:57670, ChEBI:CHEBI:58342; EC=2.3.1.13;
CC         Evidence={ECO:0000303|PubMed:22475485, ECO:0000303|PubMed:7802672};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=benzoyl-CoA + glycine = CoA + H(+) + N-benzoylglycine;
CC         Xref=Rhea:RHEA:18493, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:57369, ChEBI:CHEBI:606565;
CC         EC=2.3.1.71; Evidence={ECO:0000303|PubMed:22475485,
CC         ECO:0000303|PubMed:7802672};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=57.9 mM for benzoyl-CoA {ECO:0000269|PubMed:7802672};
CC         KM=83.7 mM for salicyl-CoA {ECO:0000269|PubMed:7802672};
CC         KM=124 mM for isovaleryl-CoA {ECO:0000269|PubMed:7802672};
CC         KM=198 mM for octanoyl-CoA {ECO:0000269|PubMed:7802672};
CC         Vmax=17.1 umol/min/mg enzyme with benzoyl-CoA as substrate
CC         {ECO:0000269|PubMed:7802672};
CC         Vmax=10.1 umol/min/mg enzyme with salicyl-CoA as substrate
CC         {ECO:0000269|PubMed:7802672};
CC         Vmax=7.64 umol/min/mg enzyme with isovaleryl-CoA as substrate
CC         {ECO:0000269|PubMed:7802672};
CC         Vmax=3.3 umol/min/mg enzyme with octanoyl-CoA as substrate
CC         {ECO:0000269|PubMed:7802672};
CC   -!- INTERACTION:
CC       Q6IB77; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-715463, EBI-10171774;
CC       Q6IB77; Q7Z3S9: NOTCH2NLA; NbExp=3; IntAct=EBI-715463, EBI-945833;
CC       Q6IB77-2; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-12142423, EBI-22310682;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:22475485}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6IB77-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6IB77-2; Sequence=VSP_024073, VSP_024074;
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in liver (at protein level)
CC       and kidney. Down-regulated in hepatocellular carcinoma and other liver
CC       cancers. {ECO:0000269|PubMed:22475485}.
CC   -!- SIMILARITY: Belongs to the glycine N-acyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB81453.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAL43174.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AF023466; AAB81453.1; ALT_INIT; mRNA.
DR   EMBL; AB665285; BAL43174.1; ALT_INIT; mRNA.
DR   EMBL; AB013093; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AP000445; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC009785; AAH09785.1; -; mRNA.
DR   EMBL; CR456927; CAG33208.1; -; mRNA.
DR   CCDS; CCDS7970.1; -. [Q6IB77-1]
DR   CCDS; CCDS7971.1; -. [Q6IB77-2]
DR   RefSeq; NP_005829.3; NM_005838.3. [Q6IB77-2]
DR   RefSeq; NP_964011.2; NM_201648.2. [Q6IB77-1]
DR   AlphaFoldDB; Q6IB77; -.
DR   SMR; Q6IB77; -.
DR   BioGRID; 115543; 6.
DR   IntAct; Q6IB77; 7.
DR   STRING; 9606.ENSP00000484592; -.
DR   DrugBank; DB00145; Glycine.
DR   iPTMnet; Q6IB77; -.
DR   PhosphoSitePlus; Q6IB77; -.
DR   BioMuta; GLYAT; -.
DR   DMDM; 311033446; -.
DR   EPD; Q6IB77; -.
DR   MassIVE; Q6IB77; -.
DR   PaxDb; Q6IB77; -.
DR   PeptideAtlas; Q6IB77; -.
DR   PRIDE; Q6IB77; -.
DR   ProteomicsDB; 66367; -. [Q6IB77-1]
DR   ProteomicsDB; 66368; -. [Q6IB77-2]
DR   Antibodypedia; 27684; 195 antibodies from 25 providers.
DR   DNASU; 10249; -.
DR   Ensembl; ENST00000278400.3; ENSP00000278400.3; ENSG00000149124.11. [Q6IB77-2]
DR   Ensembl; ENST00000344743.8; ENSP00000340200.3; ENSG00000149124.11. [Q6IB77-1]
DR   Ensembl; ENST00000529732.5; ENSP00000431688.1; ENSG00000149124.11. [Q6IB77-1]
DR   Ensembl; ENST00000611865.4; ENSP00000484592.1; ENSG00000149124.11. [Q6IB77-1]
DR   GeneID; 10249; -.
DR   KEGG; hsa:10249; -.
DR   MANE-Select; ENST00000344743.8; ENSP00000340200.3; NM_201648.3; NP_964011.2.
DR   UCSC; uc001nnb.4; human. [Q6IB77-1]
DR   CTD; 10249; -.
DR   DisGeNET; 10249; -.
DR   GeneCards; GLYAT; -.
DR   HGNC; HGNC:13734; GLYAT.
DR   HPA; ENSG00000149124; Group enriched (kidney, liver).
DR   MIM; 607424; gene.
DR   neXtProt; NX_Q6IB77; -.
DR   OpenTargets; ENSG00000149124; -.
DR   PharmGKB; PA28748; -.
DR   VEuPathDB; HostDB:ENSG00000149124; -.
DR   eggNOG; ENOG502SDQB; Eukaryota.
DR   GeneTree; ENSGT00950000183133; -.
DR   HOGENOM; CLU_060336_0_0_1; -.
DR   InParanoid; Q6IB77; -.
DR   OMA; IDDFDHY; -.
DR   OrthoDB; 1221333at2759; -.
DR   PhylomeDB; Q6IB77; -.
DR   TreeFam; TF353258; -.
DR   BRENDA; 2.3.1.13; 2681.
DR   BRENDA; 2.3.1.71; 2681.
DR   PathwayCommons; Q6IB77; -.
DR   Reactome; R-HSA-177128; Conjugation of salicylate with glycine.
DR   Reactome; R-HSA-177135; Conjugation of benzoate with glycine.
DR   Reactome; R-HSA-9749641; Aspirin ADME.
DR   SABIO-RK; Q6IB77; -.
DR   SignaLink; Q6IB77; -.
DR   BioGRID-ORCS; 10249; 7 hits in 1074 CRISPR screens.
DR   ChiTaRS; GLYAT; human.
DR   GeneWiki; GLYAT; -.
DR   GenomeRNAi; 10249; -.
DR   Pharos; Q6IB77; Tbio.
DR   PRO; PR:Q6IB77; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; Q6IB77; protein.
DR   Bgee; ENSG00000149124; Expressed in right lobe of liver and 104 other tissues.
DR   ExpressionAtlas; Q6IB77; baseline and differential.
DR   Genevisible; Q6IB77; HS.
DR   GO; GO:0005759; C:mitochondrial matrix; TAS:Reactome.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0016746; F:acyltransferase activity; TAS:ProtInc.
DR   GO; GO:0047961; F:glycine N-acyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0047962; F:glycine N-benzoyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0016410; F:N-acyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006637; P:acyl-CoA metabolic process; TAS:ProtInc.
DR   GO; GO:1901787; P:benzoyl-CoA metabolic process; IDA:UniProtKB.
DR   GO; GO:0006544; P:glycine metabolic process; IDA:UniProtKB.
DR   GO; GO:0032787; P:monocarboxylic acid metabolic process; ISS:UniProtKB.
DR   GO; GO:0009636; P:response to toxic substance; TAS:ProtInc.
DR   GO; GO:0006805; P:xenobiotic metabolic process; TAS:Reactome.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR010313; Glycine_N-acyltransferase.
DR   InterPro; IPR013652; Glycine_N-acyltransferase_C.
DR   InterPro; IPR015938; Glycine_N-acyltransferase_N.
DR   PANTHER; PTHR15298; PTHR15298; 1.
DR   Pfam; PF08444; Gly_acyl_tr_C; 1.
DR   Pfam; PF06021; Gly_acyl_tr_N; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Acyltransferase; Alternative splicing; Detoxification;
KW   Mitochondrion; Reference proteome; Transferase.
FT   CHAIN           1..296
FT                   /note="Glycine N-acyltransferase"
FT                   /id="PRO_0000281869"
FT   MOD_RES         16
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         16
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         127
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         127
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         141
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         141
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         159
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         169
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         183
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         183
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         256
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   MOD_RES         256
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XE0"
FT   VAR_SEQ         163
FT                   /note="I -> M (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10630424,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_024073"
FT   VAR_SEQ         164..296
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10630424,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_024074"
FT   VARIANT         17
FT                   /note="S -> T (in dbSNP:rs10896818)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_031294"
FT   VARIANT         156
FT                   /note="N -> S (in dbSNP:rs675815)"
FT                   /evidence="ECO:0000269|PubMed:10630424,
FT                   ECO:0000269|PubMed:15489334, ECO:0000269|Ref.3,
FT                   ECO:0000269|Ref.6"
FT                   /id="VAR_031295"
SQ   SEQUENCE   296 AA;  33924 MW;  CB9D63FA48C23FE3 CRC64;
     MMLPLQGAQM LQMLEKSLRK SLPASLKVYG TVFHINHGNP FNLKAVVDKW PDFNTVVVCP
     QEQDMTDDLD HYTNTYQIYS KDPQNCQEFL GSPELINWKQ HLQIQSSQPS LNEAIQNLAA
     IKSFKVKQTQ RILYMAAETA KELTPFLLKS KILSPNGGKP KAINQEMFKL SSMDVTHAHL
     VNKFWHFGGN ERSQRFIERC IQTFPTCCLL GPEGTPVCWD LMDQTGEMRM AGTLPEYRLH
     GLVTYVIYSH AQKLGKLGFP VYSHVDYSNE AMQKMSYTLQ HVPIPRSWNQ WNCVPL
 
 
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