ID   AMIS_PSEAE              Reviewed;         171 AA.
AC   Q51417;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   08-DEC-2000, sequence version 2.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Putative transporter protein AmiS;
GN   Name=amiS; OrderedLocusNames=PA3362;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PAC1;
RX   PubMed=7642533; DOI=10.1074/jbc.270.32.18818;
RA   Wilson S.A., Williams R.J., Pearl L.H., Drew R.E.;
RT   "Identification of two new genes in the Pseudomonas aeruginosa amidase
RT   operon, encoding an ATPase (AmiB) and a putative integral membrane protein
RT   (AmiS).";
RL   J. Biol. Chem. 270:18818-18824(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-81.
RX   PubMed=2495988; DOI=10.1016/0014-5793(89)80249-2;
RA   Lowe N., Rice P.M., Drew R.E.;
RT   "Nucleotide sequence of the aliphatic amidase regulator gene (amiR) of
RT   Pseudomonas aeruginosa.";
RL   FEBS Lett. 246:39-43(1989).
CC   -!- FUNCTION: Possible transporter that might be responsible for the
CC       adsorption of amidase substrates or release of their hydrolysis
CC       products. AmiB and AmiS may act jointly in to a two component ABC
CC       transporter system.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the AmiS/UreI family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG06750.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X77161; CAA54406.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG06750.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X13776; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A83226; A83226.
DR   PIR; S44146; S44146.
DR   RefSeq; NP_252052.1; NC_002516.2.
DR   AlphaFoldDB; Q51417; -.
DR   SMR; Q51417; -.
DR   STRING; 287.DR97_4565; -.
DR   TCDB; 1.A.29.1.1; the urea/amide channel (uac) family.
DR   PaxDb; Q51417; -.
DR   DNASU; 880572; -.
DR   EnsemblBacteria; AAG06750; AAG06750; PA3362.
DR   GeneID; 880572; -.
DR   KEGG; pae:PA3362; -.
DR   PATRIC; fig|208964.12.peg.3521; -.
DR   PseudoCAP; PA3362; -.
DR   HOGENOM; CLU_096400_1_0_6; -.
DR   OMA; FTYLWVA; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 1.25.40.600; -; 1.
DR   InterPro; IPR003211; AmiSUreI_transpt.
DR   InterPro; IPR038523; AmiSUreI_transpt_sf.
DR   Pfam; PF02293; AmiS_UreI; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..171
FT                   /note="Putative transporter protein AmiS"
FT                   /id="PRO_0000064584"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        140
FT                   /note="S -> G (in Ref. 1; CAA54406)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   171 AA;  18321 MW;  B26ACB22217AAD39 CRC64;
     MLGLVLLYVG AVLFLNAVWL LGKISGREVA VINFLVGVLS ACVAFYLIFS AAAGQGSLKA
     GALTLLFAFT YLWVAANQFL EVDGKGLGWF CLFVSLTACT VAIESFAGAS GPFGLWNAVN
     WTVWALLWFC FFLLLGLSRS IQKPVAYLTL ASAIFTAWLP GLLLLGQVLK A