AML5_ARATH
ID AML5_ARATH Reviewed; 800 AA.
AC Q8VWF5; Q0WLA0; Q9C7R1;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 136.
DE RecName: Full=Protein MEI2-like 5;
DE Short=AML5;
DE AltName: Full=MEI2-like protein 5;
GN Name=ML5; OrderedLocusNames=At1g29400; ORFNames=F15D2.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 167-800.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND DEVELOPMENTAL STAGE.
RX PubMed=15356386; DOI=10.1023/b:plan.0000040819.33383.b6;
RA Anderson G.H., Alvarez N.D., Gilman C., Jeffares D.C., Trainor V.C.,
RA Hanson M.R., Veit B.;
RT "Diversification of genes encoding mei2 -like RNA binding proteins in
RT plants.";
RL Plant Mol. Biol. 54:653-670(2004).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15720729; DOI=10.1186/1471-2229-5-2;
RA Anderson G.H., Hanson M.R.;
RT "The Arabidopsis Mei2 homologue AML1 binds AtRaptor1B, the plant homologue
RT of a major regulator of eukaryotic cell growth.";
RL BMC Plant Biol. 5:2-2(2005).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16473967; DOI=10.1105/tpc.105.039156;
RA Kaur J., Sebastian J., Siddiqi I.;
RT "The Arabidopsis-mei2-like genes play a role in meiosis and vegetative
RT growth in Arabidopsis.";
RL Plant Cell 18:545-559(2006).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-789 AND SER-792, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384; SER-390 AND SER-792, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Probable RNA-binding protein that plays a role in meiosis and
CC vegetative growth. {ECO:0000269|PubMed:15720729,
CC ECO:0000269|PubMed:16473967}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout the meristem during embryonic
CC and vegetative development. Expressed in floral organogenic regions.
CC {ECO:0000269|PubMed:15356386}.
CC -!- DISRUPTION PHENOTYPE: Early flowering. {ECO:0000269|PubMed:15720729}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51742.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF02107.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC068667; AAG51742.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31082.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31083.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58035.1; -; Genomic_DNA.
DR EMBL; AY062613; AAL32691.1; -; mRNA.
DR EMBL; AY070368; AAL49866.1; -; mRNA.
DR EMBL; AY091327; AAM14266.1; -; mRNA.
DR EMBL; AK230306; BAF02107.1; ALT_INIT; mRNA.
DR PIR; F86416; F86416.
DR RefSeq; NP_001320501.1; NM_001332851.1.
DR RefSeq; NP_174233.2; NM_102680.2.
DR RefSeq; NP_849727.1; NM_179396.3.
DR AlphaFoldDB; Q8VWF5; -.
DR SMR; Q8VWF5; -.
DR BioGRID; 25051; 3.
DR IntAct; Q8VWF5; 3.
DR STRING; 3702.AT1G29400.2; -.
DR iPTMnet; Q8VWF5; -.
DR PaxDb; Q8VWF5; -.
DR PRIDE; Q8VWF5; -.
DR ProteomicsDB; 244904; -.
DR EnsemblPlants; AT1G29400.1; AT1G29400.1; AT1G29400.
DR EnsemblPlants; AT1G29400.2; AT1G29400.2; AT1G29400.
DR EnsemblPlants; AT1G29400.3; AT1G29400.3; AT1G29400.
DR GeneID; 839816; -.
DR Gramene; AT1G29400.1; AT1G29400.1; AT1G29400.
DR Gramene; AT1G29400.2; AT1G29400.2; AT1G29400.
DR Gramene; AT1G29400.3; AT1G29400.3; AT1G29400.
DR KEGG; ath:AT1G29400; -.
DR Araport; AT1G29400; -.
DR TAIR; locus:2013583; AT1G29400.
DR eggNOG; KOG4660; Eukaryota.
DR HOGENOM; CLU_012447_0_0_1; -.
DR InParanoid; Q8VWF5; -.
DR OMA; FSSYRMM; -.
DR OrthoDB; 1464915at2759; -.
DR PhylomeDB; Q8VWF5; -.
DR PRO; PR:Q8VWF5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8VWF5; baseline and differential.
DR Genevisible; Q8VWF5; AT.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0045927; P:positive regulation of growth; IMP:UniProtKB.
DR GO; GO:0045836; P:positive regulation of meiotic nuclear division; IMP:UniProtKB.
DR CDD; cd12524; RRM1_MEI2_like; 1.
DR CDD; cd12531; RRM3_MEI2_like; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR034453; MEI2-like_RRM1.
DR InterPro; IPR034454; MEI2-like_RRM3.
DR InterPro; IPR007201; Mei2-like_Rrm_C.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR Pfam; PF04059; RRM_2; 1.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Meiosis; Phosphoprotein; Reference proteome; Repeat; RNA-binding.
FT CHAIN 1..800
FT /note="Protein MEI2-like 5"
FT /id="PRO_0000409345"
FT DOMAIN 168..241
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 253..326
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 470..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 776..800
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 384
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT MOD_RES 390
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT MOD_RES 789
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862"
FT MOD_RES 792
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862,
FT ECO:0007744|PubMed:19376835"
SQ SEQUENCE 800 AA; 88212 MW; E87C54AAAACA8E48 CRC64;
MDIPHEAEAG AWGILPGFGR HHHPSSDATL FSSSLPVFPR GKLQLSDNRD GFSLIDDTAV
SRTNKFNESA DDFESHSIGN LLPDEEDLLT GMMDDLDLGE LPDADDYDLF GSGGGMELDA
DFRDNLSMSG PPRLSLSSLG GNAIPQFNIP NGAGTVAGEH PYGEHPSRTL FVRNINSNVE
DSELTALFEQ YGDIRTLYTT CKHRGFVMIS YYDIRSARMA MRSLQNKPLR RRKLDIHFSI
PKDNPSEKDM NQGTLVVFNL DPSISNDDLH GIFGAHGEIK EIRETPHKRH HKFVEFYDVR
GAEAALKALN RCEIAGKRIK VEPSRPGGAR RSLMLQLNQD LENDDLHYLP MIGSPMANSP
PMQGNWPLNS PVEGSPLQSV LSRSPVFGLS PTRNGHLSGL ASALNSQGPS SKLAPIGRGQ
IGSNGFQQSS HLFQEPKMDN KYTGNLSPSG PLISNGGGIE TLSGSEFLWG SPNARSEPSS
SSVWSTSSTG NPLFSTRVDR SVPFPHQHQN QSRSHHHFHV GSAPSGVPLE KHFGFVPESS
KDALFMNTVG LQGMSGMGLN GGSFSSKMAN NGIINSGSMA ENGFSSYRMM SSPRFSPMFL
SSGLNPGRFA SGFDSLYENG RPRRVENNSN QVESRKQFQL DLEKILNGED SRTTLMIKNI
PNKYTSKMLL AAIDEKNQGT YNFLYLPIDF KNKCNVGYAF INMLNPELII PFYEAFNGKK
WEKFNSEKVA SLAYARIQGK SALIAHFQNS SLMNEDMRCR PIIFDTPNNP ESVEQVVDEE
SKNMDLLDSQ LSDDDGRERS
