ID   AMML_ASTMO              Reviewed;          15 AA.
AC   P85004;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Antifungal lectin AMML;
DE   Flags: Fragment;
OS   Astragalus mongholicus (Milk vetch) (Astragalus membranaceus var.
OS   mongholicus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Galegeae; Astragalus.
OX   NCBI_TaxID=119829;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, AND GLYCOSYLATION.
RX   PubMed=16140255; DOI=10.1016/j.abb.2005.07.019;
RA   Yan Q., Jiang Z., Yang S., Deng W., Han L.;
RT   "A novel homodimeric lectin from Astragalus mongholicus with antifungal
RT   activity.";
RL   Arch. Biochem. Biophys. 442:72-81(2005).
CC   -!- FUNCTION: Lectin that binds strongly to galactose, lactose, D-
CC       raffinose, L-rhamnose and cellobiose, and less strongly to D-glucose,
CC       D-xylose and L-arabinose. Does not bind D-galacturonic acid, D-
CC       fructose, D-mannose, D-ribose, L-sorbose, maltose and sucrose. Has
CC       hemagglutinating activity towards human type O and rabbit erythrocytes.
CC       Has strong antifungal activity against B.cinera with an IC(50) value of
CC       1.2 uM, and weaker antifungal activity against D.turcia, F.oxysporum
CC       and Colletorichum sp. Lacks antifungal activity against F.oxysporum and
CC       M.arachidicola. {ECO:0000269|PubMed:16140255}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:16140255}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:16140255}.
CC   -!- MISCELLANEOUS: Hemagglutinating activity stable when incubated at 65
CC       degrees Celsius for 30 minutes. Hemagglutinating activity is reduced by
CC       50% when incubated at 75 degrees Celsius for 30 minutes and is
CC       abolished by incubation at 85 degrees Celsius for 30 minutes.
CC       Hemagglutinating activity is stable between pH 4.5 and 7.5, but is
CC       completely inhibited by incubation below pH 3.0 or above pH 10.
CC       {ECO:0000269|PubMed:16140255}.
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DR   GO; GO:0005534; F:galactose binding; IDA:UniProtKB.
DR   GO; GO:0005536; F:glucose binding; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Fungicide; Glycoprotein;
KW   Hemagglutinin; Lectin; Plant defense.
FT   CHAIN           1..>15
FT                   /note="Antifungal lectin AMML"
FT                   /id="PRO_0000253931"
FT   NON_TER         15
FT                   /evidence="ECO:0000303|PubMed:16140255"
SQ   SEQUENCE   15 AA;  1517 MW;  3B4F6D7B4B7050B9 CRC64;
     ESGINLQGDA TLANN