ID   AMND_PSESP              Reviewed;         142 AA.
AC   Q9KWS2;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=2-aminomuconate deaminase;
DE            EC=3.5.99.5;
GN   Name=amnD;
OS   Pseudomonas sp.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=306;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=AP-3;
RX   PubMed=9169437; DOI=10.1074/jbc.272.23.14727;
RA   Takenaka S., Murakami S., Shinke R., Hatakeyama K., Yukawa H., Aoki K.;
RT   "Novel genes encoding 2-aminophenol 1,6-dioxygenase from Pseudomonas
RT   species AP-3 growing on 2-aminophenol and catalytic properties of the
RT   purified enzyme.";
RL   J. Biol. Chem. 272:14727-14732(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, CATALYTIC
RP   ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, SUBUNIT,
RP   AND ACTIVITY REGULATION.
RC   STRAIN=AP-3;
RX   PubMed=11081795; DOI=10.1007/s002030000203;
RA   Takenaka S., Murakami S., Kim Y.J., Aoki K.;
RT   "Complete nucleotide sequence and functional analysis of the genes for 2-
RT   aminophenol metabolism from Pseudomonas sp. AP-3.";
RL   Arch. Microbiol. 174:265-272(2000).
CC   -!- FUNCTION: Involved in the modified meta-cleavage pathway for the 2-
CC       aminophenol catabolism. Only active toward 2-aminomuconic acid.
CC       {ECO:0000269|PubMed:9169437}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-aminomuconate + H2O = (3E)-2-oxohex-3-enedioate +
CC         NH4(+); Xref=Rhea:RHEA:20996, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:64908, ChEBI:CHEBI:77859; EC=3.5.99.5;
CC         Evidence={ECO:0000269|PubMed:11081795};
CC   -!- ACTIVITY REGULATION: Slightly inhibited by Pb(2+), Hg(+) and Cu(2+).
CC       {ECO:0000269|PubMed:11081795}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7.0. Stable in the range of pH 5.5-8.0.
CC         {ECO:0000269|PubMed:11081795};
CC       Temperature dependence:
CC         Stable up to 55 degrees Celsius. {ECO:0000269|PubMed:11081795};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:11081795}.
CC   -!- SIMILARITY: Belongs to the 2-aminomuconate deaminase family.
CC       {ECO:0000305}.
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DR   EMBL; AB020521; BAB03536.1; -; Genomic_DNA.
DR   PDB; 6IZH; X-ray; 1.75 A; A/B/C/D/E/F/G/H/I=1-142.
DR   PDBsum; 6IZH; -.
DR   AlphaFoldDB; Q9KWS2; -.
DR   SMR; Q9KWS2; -.
DR   KEGG; ag:BAB03536; -.
DR   BioCyc; MetaCyc:MON-14742; -.
DR   GO; GO:0050540; F:2-aminomuconate deaminase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1330.40; -; 1.
DR   InterPro; IPR035959; RutC-like_sf.
DR   InterPro; IPR006175; YjgF/YER057c/UK114.
DR   PANTHER; PTHR11803; PTHR11803; 1.
DR   Pfam; PF01042; Ribonuc_L-PSP; 1.
DR   SUPFAM; SSF55298; SSF55298; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aromatic hydrocarbons catabolism; Direct protein sequencing;
KW   Hydrolase.
FT   CHAIN           1..142
FT                   /note="2-aminomuconate deaminase"
FT                   /id="PRO_0000383028"
FT   STRAND          19..21
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   STRAND          24..28
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   STRAND          31..37
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   HELIX           61..78
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   HELIX           83..85
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   STRAND          86..94
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   HELIX           96..98
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   HELIX           99..106
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   TURN            107..109
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   STRAND          116..121
FT                   /evidence="ECO:0007829|PDB:6IZH"
FT   STRAND          131..139
FT                   /evidence="ECO:0007829|PDB:6IZH"
SQ   SEQUENCE   142 AA;  15533 MW;  762ECDEF3B376E36 CRC64;
     MVSKADNSAK LVEGKAKPMG SFPHVKRAGD FLFVSGTSSR RPDNTFVGAE PDDTGRPRPN
     IELQTREVIS NIRDILQSVG ADLGDVVEVC SYLVNMNDFA AYNKVYAEFF DATGPARTTV
     AVHQLPHPQL VIEIKVVAYK PL