AMNLS_DROME
ID AMNLS_DROME Reviewed; 505 AA.
AC Q9VPN2;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Protein amnionless {ECO:0000305};
DE Flags: Precursor;
GN Name=Amnionless {ECO:0000312|FlyBase:FBgn0025686};
GN ORFNames=CG11592 {ECO:0000312|FlyBase:FBgn0025686};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAY55609.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Celniker S.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=23264686; DOI=10.1681/asn.2012080795;
RA Zhang F., Zhao Y., Chao Y., Muir K., Han Z.;
RT "Cubilin and amnionless mediate protein reabsorption in Drosophila
RT nephrocytes.";
RL J. Am. Soc. Nephrol. 24:209-216(2013).
CC -!- FUNCTION: Required in the nephrocyte for normal uptake of proteins and
CC elimination of toxins, and for maintenance of endocytic trafficking
CC structures. May function together with Cubn.
CC {ECO:0000269|PubMed:23264686}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:23264686};
CC Single-pass type I membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in nephrocytes.
CC {ECO:0000269|PubMed:23264686}.
CC -!- DEVELOPMENTAL STAGE: Detected only in garland nephrocytes at the
CC embryonic stage. Highly expressed in both garland and pericardial
CC nephrocytes at the larval stage. {ECO:0000269|PubMed:23264686}.
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DR EMBL; AE014134; AAF51514.1; -; Genomic_DNA.
DR EMBL; AE014134; AGB92350.1; -; Genomic_DNA.
DR EMBL; BT023193; AAY55609.1; -; mRNA.
DR RefSeq; NP_001259813.1; NM_001272884.1.
DR RefSeq; NP_608515.1; NM_134671.2.
DR AlphaFoldDB; Q9VPN2; -.
DR SMR; Q9VPN2; -.
DR IntAct; Q9VPN2; 4.
DR STRING; 7227.FBpp0303497; -.
DR PaxDb; Q9VPN2; -.
DR PRIDE; Q9VPN2; -.
DR DNASU; 33199; -.
DR EnsemblMetazoa; FBtr0078134; FBpp0077793; FBgn0025686.
DR EnsemblMetazoa; FBtr0330647; FBpp0303497; FBgn0025686.
DR GeneID; 33199; -.
DR KEGG; dme:Dmel_CG11592; -.
DR UCSC; CG11592-RA; d. melanogaster.
DR CTD; 33199; -.
DR FlyBase; FBgn0025686; Amnionless.
DR VEuPathDB; VectorBase:FBgn0025686; -.
DR eggNOG; ENOG502QUUQ; Eukaryota.
DR GeneTree; ENSGT00390000007463; -.
DR HOGENOM; CLU_539993_0_0_1; -.
DR InParanoid; Q9VPN2; -.
DR OMA; GCHKDFD; -.
DR OrthoDB; 827066at2759; -.
DR PhylomeDB; Q9VPN2; -.
DR Reactome; R-DME-9758881; Uptake of dietary cobalamins into enterocytes.
DR BioGRID-ORCS; 33199; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33199; -.
DR PRO; PR:Q9VPN2; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0025686; Expressed in excretory cell and 13 other tissues.
DR GO; GO:0016324; C:apical plasma membrane; IBA:GO_Central.
DR GO; GO:0030139; C:endocytic vesicle; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0043235; C:receptor complex; IPI:FlyBase.
DR GO; GO:0038024; F:cargo receptor activity; IDA:FlyBase.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IMP:FlyBase.
DR GO; GO:0097206; P:nephrocyte filtration; IMP:FlyBase.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006898; P:receptor-mediated endocytosis; IBA:GO_Central.
DR GO; GO:0097017; P:renal protein absorption; IMP:FlyBase.
DR InterPro; IPR026112; AMN.
DR PANTHER; PTHR14995; PTHR14995; 1.
DR Pfam; PF14828; Amnionless; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Protein transport; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..505
FT /note="Protein amnionless"
FT /id="PRO_5006753003"
FT TOPO_DOM 21..344
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 366..505
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 451..482
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 455..482
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 505 AA; 56864 MW; 02444A1084799BAC CRC64;
MGLHWQWLIW ALVGLHVALA TKWYGGGMDF NDPTAWLDDH LPCAQDLVVF PEYYPALLPL
PEDISIDGLV FPREGAILLA EESTITFGSD KQPSCESDEN KAYLKPPKSS KWFDPGTWTD
KSKEFSTFTP ELERIPCDDE QVIIKGHGPL AFDLENVQYL RLGQLILAGS SISKTYLEQL
ISRDLGQFLF YNAEYVQVEY YRGELCGCHK DFERLLEPVC HNVQEQCETP HCLSPVRPLG
SCCLICGAIL STPTTHCTEG SRKELVAQIS NLISQESLKD QIGLHVEFVG SQKFGNCLQA
VVTDRHKYSE RSLEFLQQNE HNWNKTGTTL KVSGRPYNPN VSFSSIVLIL FCMALVGLVS
VVILAHFMPE NPYLNRIPQW IHDPRLWRWR HLGLRLRRNL LFNRFDNGGA EGGSSEGGAS
GVDRLGIMAY DPESGEVRER AFDNPMFEQG GALEEAAKES QEQDEILSVP KMETGDLDAR
SVVDEQELTE INLETCEADT DEETI
