AMN_DROME
ID AMN_DROME Reviewed; 180 AA.
AC Q24049; Q9VWC1;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 3.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Amnesiac neuropeptides;
DE Contains:
DE RecName: Full=Amnesiac peptide 24;
DE Contains:
DE RecName: Full=Amnesiac peptide 30;
DE Contains:
DE RecName: Full=Amnesiac peptide 56;
DE Flags: Precursor;
GN Name=amn; ORFNames=CG11937;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Canton-S, and Oregon-R; TISSUE=Head;
RX PubMed=7754370; DOI=10.1126/science.7754370;
RA Feany M.B., Quinn W.G.;
RT "A neuropeptide gene defined by the Drosophila memory mutant amnesiac.";
RL Science 268:869-872(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10516293; DOI=10.1523/jneurosci.19-20-08740.1999;
RA DeZazzo J., Xia S., Christensen J., Velinzon K., Tully T.;
RT "Developmental expression of an amn(+) transgene rescues the mutant memory
RT defect of amnesiac adults.";
RL J. Neurosci. 19:8740-8746(1999).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11114336; DOI=10.1016/s0092-8674(00)00183-5;
RA Waddell S., Armstrong J.D., Kitamoto T., Kaiser K., Quinn W.G.;
RT "The amnesiac gene product is expressed in two neurons in the Drosophila
RT brain that are critical for memory.";
RL Cell 103:805-813(2000).
RN [6]
RP FUNCTION.
RX PubMed=11517334; DOI=10.1073/pnas.191107698;
RA Yager J., Richards S., Hekmat-Scafe D.S., Hurd D.D., Sundaresan V.,
RA Caprette D.R., Saxton W.M., Carlson J.R., Stern M.;
RT "Control of Drosophila perineurial glial growth by interacting
RT neurotransmitter-mediated signaling pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:10445-10450(2001).
RN [7]
RP PROTEOLYTIC PROCESSING, AND DISRUPTION PHENOTYPE.
RX PubMed=12270109; DOI=10.1016/s0006-291x(02)02144-7;
RA Hashimoto H., Shintani N., Baba A.;
RT "Higher brain functions of PACAP and a homologous Drosophila memory gene
RT amnesiac: insights from knockouts and mutants.";
RL Biochem. Biophys. Res. Commun. 297:427-431(2002).
CC -!- FUNCTION: Required for associative learning and memory in adults.
CC Expression pattern suggests a modulatory role in memory formation.
CC Controls neurotransmitter-mediated signaling pathways associated with
CC the structure of the larval peripheral nerve.
CC {ECO:0000269|PubMed:10516293, ECO:0000269|PubMed:11114336,
CC ECO:0000269|PubMed:11517334}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Enriched expression in the embryonic and larval
CC nervous systems. Strongly expressed in two large neurons that project
CC over all the lobes of the mushroom bodies.
CC {ECO:0000269|PubMed:10516293, ECO:0000269|PubMed:11114336}.
CC -!- DISRUPTION PHENOTYPE: Increased growth of the perineurial glial layer
CC of the larval peripheral nerve. {ECO:0000269|PubMed:12270109}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC46587.2; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U22825; AAC46587.2; ALT_FRAME; mRNA.
DR EMBL; AE014298; AAF49025.1; -; Genomic_DNA.
DR RefSeq; NP_523416.2; NM_078692.2.
DR AlphaFoldDB; Q24049; -.
DR BioGRID; 59293; 7.
DR STRING; 7227.FBpp0074571; -.
DR PaxDb; Q24049; -.
DR DNASU; 32999; -.
DR EnsemblMetazoa; FBtr0074802; FBpp0074571; FBgn0086782.
DR GeneID; 32999; -.
DR KEGG; dme:Dmel_CG11937; -.
DR CTD; 81693; -.
DR FlyBase; FBgn0086782; amn.
DR VEuPathDB; VectorBase:FBgn0086782; -.
DR HOGENOM; CLU_1497785_0_0_1; -.
DR OrthoDB; 1825909at2759; -.
DR BioGRID-ORCS; 32999; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32999; -.
DR PRO; PR:Q24049; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0086782; Expressed in brain and 10 other tissues.
DR ExpressionAtlas; Q24049; baseline.
DR Genevisible; Q24049; DM.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0005184; F:neuropeptide hormone activity; ISS:FlyBase.
DR GO; GO:0007190; P:activation of adenylate cyclase activity; TAS:FlyBase.
DR GO; GO:0007615; P:anesthesia-resistant memory; TAS:FlyBase.
DR GO; GO:0048149; P:behavioral response to ethanol; IMP:FlyBase.
DR GO; GO:0048266; P:behavioral response to pain; IMP:FlyBase.
DR GO; GO:0019933; P:cAMP-mediated signaling; TAS:FlyBase.
DR GO; GO:0007619; P:courtship behavior; TAS:FlyBase.
DR GO; GO:0007611; P:learning or memory; IMP:FlyBase.
DR GO; GO:0007616; P:long-term memory; TAS:FlyBase.
DR GO; GO:0008049; P:male courtship behavior; TAS:FlyBase.
DR GO; GO:0072375; P:medium-term memory; IMP:FlyBase.
DR GO; GO:0007613; P:memory; IMP:FlyBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEP:FlyBase.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0008355; P:olfactory learning; IDA:FlyBase.
DR GO; GO:0042066; P:perineurial glial growth; IMP:FlyBase.
DR GO; GO:0009408; P:response to heat; IMP:FlyBase.
DR GO; GO:0040040; P:thermosensory behavior; IMP:FlyBase.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Developmental protein; Differentiation;
KW Neurogenesis; Neuropeptide; Neurotransmitter transport; Reference proteome;
KW Secreted; Signal; Transport.
FT SIGNAL 1..32
FT /evidence="ECO:0000255"
FT PROPEP 33..35
FT /id="PRO_0000020704"
FT PEPTIDE 36..59
FT /note="Amnesiac peptide 24"
FT /evidence="ECO:0000255"
FT /id="PRO_0000020705"
FT PEPTIDE 62..93
FT /note="Amnesiac peptide 30"
FT /evidence="ECO:0000255"
FT /id="PRO_0000020706"
FT PEPTIDE 96..151
FT /note="Amnesiac peptide 56"
FT /evidence="ECO:0000255"
FT /id="PRO_0000020707"
FT PROPEP 152..180
FT /id="PRO_0000020708"
FT REGION 155..180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 180 AA; 20399 MW; C44CF9C0844D75A8 CRC64;
MRSFCCCFYP AAVALHCVLL FYTFFLLFRA SALRRRVVSG SKGSAALALC RQFEQLSASR
RERAEECRTT QLRYHYHRNG AQSRSLCAAV LCCKRSYIPR PNFSCFSLVF PVGQRFAAAR
TRFGPTLVAS WPLCNDSETK VLTKWPSCSL IGRRSVPRGQ PKFSRENPRA LSPSLLGEMR
