ID   AMO2_AMOLO              Reviewed;          62 AA.
AC   C5H0C8;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Amolopin-P2 {ECO:0000303|PubMed:18312859, ECO:0000312|EMBL:ACA09631.1};
DE   Flags: Precursor;
OS   Amolops loloensis (Lolokou Sucker Frog) (Staurois loloensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Amolops.
OX   NCBI_TaxID=318551;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SYNTHESIS OF 45-62.
RC   TISSUE=Skin;
RX   PubMed=18312859; DOI=10.1016/j.biochi.2008.02.003;
RA   Wang A., Wang J., Hong J., Feng H., Yang H., Yu X., Ma Y., Lai R.;
RT   "A novel family of antimicrobial peptides from the skin of Amolops
RT   loloensis.";
RL   Biochimie 90:863-867(2008).
CC   -!- FUNCTION: Antimicrobial peptide with activity against Gram-positive
CC       bacteria (PubMed:18312859). Has been tested against S.aureus (MIC=37.5
CC       ug/mL), against B.pumilus (MIC=75.0 ug/mL), B.cereus (no activity
CC       detected) (PubMed:18312859). Does not show activity against Gram-
CC       negative bacteria (E.coli, B.dysenteriae, A.calcoaceticus,
CC       P.aeruginosa) and fungi (C.albicans) (PubMed:18312859). Does not show
CC       hemolytic activity against rabbit erythrocytes (PubMed:18312859).
CC       {ECO:0000269|PubMed:18312859}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:18312859}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:18312859}.
CC   -!- MISCELLANEOUS: Mainly adopts a combination conformation of random and
CC       beta-sheet. {ECO:0000269|PubMed:18312859}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Amolopin subfamily. {ECO:0000305}.
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DR   EMBL; EU311541; ACA09631.1; -; mRNA.
DR   AlphaFoldDB; C5H0C8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   3: Inferred from homology;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Cleavage on pair of basic residues; Immunity; Innate immunity; Secreted;
KW   Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..44
FT                   /evidence="ECO:0000305|PubMed:18312859"
FT                   /id="PRO_0000450013"
FT   PEPTIDE         45..62
FT                   /note="Amolopin-P2"
FT                   /evidence="ECO:0000305|PubMed:18312859"
FT                   /id="PRO_5002952197"
SQ   SEQUENCE   62 AA;  6838 MW;  BDFC59CDDA41E113 CRC64;
     MFTLKKSLLL LFFLGTISLS LCEQERGADE EENGGEVTEQ EVKRNVLSSV ANGINRALSF
     FG