AMOL2_DANRE
ID AMOL2_DANRE Reviewed; 721 AA.
AC A1YB07; B3DIZ1; F1QMD1;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2012, sequence version 2.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Angiomotin-like 2a;
GN Name=amotl2a; Synonyms=amotl2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SRC, DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RX PubMed=17293535; DOI=10.1242/dev.02782;
RA Huang H., Lu F.I., Jia S., Meng S., Cao Y., Wang Y., Ma W., Yin K., Wen Z.,
RA Peng J., Thisse C., Thisse B., Meng A.;
RT "Amotl2 is essential for cell movements in zebrafish embryo and regulates
RT c-Src translocation.";
RL Development 134:979-988(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, PHOSPHORYLATION AT TYR-103, AND MUTAGENESIS OF TYR-103.
RX PubMed=21937427; DOI=10.1074/jbc.m111.296806;
RA Wang Y., Li Z., Xu P., Huang L., Tong J., Huang H., Meng A.;
RT "Angiomotin-like2 gene (amotl2) is required for migration and proliferation
RT of endothelial cells during angiogenesis.";
RL J. Biol. Chem. 286:41095-41104(2011).
RN [5]
RP FUNCTION, INTERACTION WITH SRC, AND SUBCELLULAR LOCATION.
RX PubMed=22362771; DOI=10.1074/jbc.m112.347419;
RA Li Z., Wang Y., Zhang M., Xu P., Huang H., Wu D., Meng A.;
RT "The Amotl2 gene inhibits Wnt/beta-catenin signaling and regulates
RT embryonic development in zebrafish.";
RL J. Biol. Chem. 287:13005-13015(2012).
CC -!- FUNCTION: Regulates the translocation of phosphorylated SRC to
CC peripheral cell-matrix adhesion sites. Required for proper architecture
CC of actin filaments and for cell movements during embryogenesis.
CC Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting
CC CTNNB1 to recycling endosomes and hence preventing its translocation to
CC the nucleus. Participates in angiogenesis. May play a role in the
CC polarity, proliferation and migration of endothelial cells. Selectively
CC promotes FGF-induced MAPK activation through SRC.
CC {ECO:0000269|PubMed:17293535, ECO:0000269|PubMed:21937427,
CC ECO:0000269|PubMed:22362771}.
CC -!- SUBUNIT: Interacts with SRC. {ECO:0000269|PubMed:17293535,
CC ECO:0000269|PubMed:22362771}.
CC -!- SUBCELLULAR LOCATION: Recycling endosome {ECO:0000269|PubMed:22362771}.
CC -!- DEVELOPMENTAL STAGE: Detected before the 1k-cell stage, suggesting that
CC it is maternally supplied. At the sphere stage, stronger expression in
CC the dorsal blastomeres. In the gastrula, expressed in the whole embryo,
CC except in the evacuation zone. During segmentation and pharyngula
CC period, expressed in many distinct domains, including the polster,
CC telencephalon, trigeminal placodes, rhombomeres, trunk neurons, somites
CC and axial vasculature. During the pharyngula period, additional
CC expression observed in lateral line primordia and in intersegmental
CC vessels. {ECO:0000269|PubMed:17293535}.
CC -!- INDUCTION: Up-regulated by fgf17 and fgf8.
CC {ECO:0000269|PubMed:17293535}.
CC -!- PTM: Phosphorylation at Tyr-103 is necessary for efficient binding to
CC SRC and synergistically functioning with SRC to activate the downstream
CC MAPK pathway. {ECO:0000269|PubMed:21937427}.
CC -!- SIMILARITY: Belongs to the angiomotin family. {ECO:0000305}.
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DR EMBL; DQ887096; ABI74626.1; -; mRNA.
DR EMBL; CR626940; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC163299; AAI63299.1; -; mRNA.
DR RefSeq; NP_001073646.1; NM_001080177.1.
DR AlphaFoldDB; A1YB07; -.
DR SMR; A1YB07; -.
DR STRING; 7955.ENSDARP00000083347; -.
DR iPTMnet; A1YB07; -.
DR PaxDb; A1YB07; -.
DR PeptideAtlas; A1YB07; -.
DR PRIDE; A1YB07; -.
DR Ensembl; ENSDART00000088914; ENSDARP00000083347; ENSDARG00000061923.
DR Ensembl; ENSDART00000160354; ENSDARP00000134595; ENSDARG00000061923.
DR GeneID; 558920; -.
DR KEGG; dre:558920; -.
DR CTD; 558920; -.
DR ZFIN; ZDB-GENE-030131-9770; amotl2a.
DR eggNOG; ENOG502QR7W; Eukaryota.
DR GeneTree; ENSGT00940000156577; -.
DR HOGENOM; CLU_009937_2_0_1; -.
DR InParanoid; A1YB07; -.
DR OMA; YRFYQPQ; -.
DR OrthoDB; 369983at2759; -.
DR PhylomeDB; A1YB07; -.
DR TreeFam; TF333368; -.
DR Reactome; R-DRE-2028269; Signaling by Hippo.
DR PRO; PR:A1YB07; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 6.
DR Bgee; ENSDARG00000061923; Expressed in somite and 56 other tissues.
DR ExpressionAtlas; A1YB07; baseline and differential.
DR GO; GO:0005923; C:bicellular tight junction; IBA:GO_Central.
DR GO; GO:0005938; C:cell cortex; IDA:ZFIN.
DR GO; GO:0030054; C:cell junction; IDA:ZFIN.
DR GO; GO:0005911; C:cell-cell junction; IDA:ZFIN.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0099513; C:polymeric cytoskeletal fiber; IDA:ZFIN.
DR GO; GO:0055037; C:recycling endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0030036; P:actin cytoskeleton organization; IGI:ZFIN.
DR GO; GO:0001525; P:angiogenesis; IMP:ZFIN.
DR GO; GO:0016477; P:cell migration; IMP:ZFIN.
DR GO; GO:0060026; P:convergent extension; IMP:ZFIN.
DR GO; GO:0030866; P:cortical actin cytoskeleton organization; IMP:ZFIN.
DR GO; GO:0035907; P:dorsal aorta development; IGI:ZFIN.
DR GO; GO:0035912; P:dorsal aorta morphogenesis; IMP:ZFIN.
DR GO; GO:0009880; P:embryonic pattern specification; IMP:ZFIN.
DR GO; GO:0001935; P:endothelial cell proliferation; IMP:ZFIN.
DR GO; GO:0003365; P:establishment of cell polarity involved in ameboidal cell migration; IBA:GO_Central.
DR GO; GO:0051649; P:establishment of localization in cell; IGI:ZFIN.
DR GO; GO:0046847; P:filopodium assembly; IMP:ZFIN.
DR GO; GO:0030334; P:regulation of cell migration; IBA:GO_Central.
DR GO; GO:0008360; P:regulation of cell shape; IGI:ZFIN.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR009114; Angiomotin.
DR InterPro; IPR024646; Angiomotin_C.
DR Pfam; PF12240; Angiomotin_C; 1.
DR PRINTS; PR01807; ANGIOMOTIN.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; Phosphoprotein; Reference proteome;
KW Wnt signaling pathway.
FT CHAIN 1..721
FT /note="Angiomotin-like 2a"
FT /id="PRO_0000418839"
FT REGION 35..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 169..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 554..575
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 666..709
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 275..531
FT /evidence="ECO:0000255"
FT MOTIF 718..721
FT /note="PDZ-binding"
FT COMPBIAS 35..83
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 554..571
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 690..709
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 103
FT /note="Phosphotyrosine; by FGFR1"
FT /evidence="ECO:0000269|PubMed:21937427"
FT MUTAGEN 103
FT /note="Y->F: Loss of phosphorylation. Drastic reduction in
FT SRC-binding and in activation of MAPK."
FT /evidence="ECO:0000269|PubMed:21937427"
FT CONFLICT 124
FT /note="Q -> H (in Ref. 1; ABI74626 and 3; AAI63299)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="A -> G (in Ref. 3; AAI63299)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="T -> S (in Ref. 3; AAI63299)"
FT /evidence="ECO:0000305"
FT CONFLICT 194
FT /note="Q -> K (in Ref. 3; AAI63299)"
FT /evidence="ECO:0000305"
FT CONFLICT 683
FT /note="A -> V (in Ref. 3; AAI63299)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 721 AA; 81711 MW; 650F7F103BC4D6C9 CRC64;
MRTAEESSGT VLHRLIQEQL RYGNPTDPTL LAIQQQALRG GSSGGGAGSP RSSLESLTQE
ESLSPQLSAR QEPQGQEHQG DFQHSESPVC HLYQLHTEEL PTYEEAKAHS QYLAYQRGQI
GLHQGSLESP GGVGGAEQDD SMWDAKREHA RSLSERLLQL SLERNCAHDN IPMSSSHSYP
QLSNNHSDTV VNEQSVHQPD QRGPPPEYPF MVRSPGYMLS HSQEHGHYYN EPPPAFHSQH
YRLFPTQPQA PRHNGLPTLT PAGQDVNVGG YSIPANNFQM EQLIKENERL KREVDSYSEK
AARLQKLEQE IQRISEAYET LMKGSAKREA LEKTMRNKLE SEIKRLHDFN RDLRDRLETA
NKQRAAIEVE DKSRHAFAKL VEQNEDHLRE RERLEKETQH LRASGEEWKR RREALEQALI
TAQTRNRQLE EELRRKRAYV EKVERMQSAL AQLQAACEKR EALELRLRTR LEQELKSLRA
QQWQAQTQHA SPGSYLDLNV SSLQQQLRER EEQVLALEAD ITRWEQKYLE ESTMRQFAMD
AAATAAAQRD TTIINHSPRN SPNSSFNEDL PSPNHRHQEM ENRIRALYAQ LLEKDAIIKV
MQQRSRREQG RPELQGLRPA RSVPSINTVA TASTTRAKGK SLSDDQTAVA SLPPLPHLLA
KIQCRDSSTQ CDSEEPSCKA EPADVAVSAP EPSTASSSES TSLKTTQISS AVENDMVEIL
I
