AMOS_DROME
ID AMOS_DROME Reviewed; 198 AA.
AC Q9Y0A7; Q0IGQ5; Q9VJ76;
DT 05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 05-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Basic helix-loop-helix transcription factor amos;
DE AltName: Full=Absent MD neurons and olfactory sensilla protein;
DE Short=Amos protein;
DE AltName: Full=Reduced olfactory organs protein;
DE AltName: Full=Rough eye protein;
GN Name=amos; Synonyms=Roi, rolo; ORFNames=CG10393;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-R;
RX PubMed=10707973; DOI=10.1016/s0896-6273(00)80872-7;
RA Goulding S.E., zur Lage P., Jarman A.P.;
RT "Amos, a proneural gene for Drosophila olfactory sense organs that is
RT regulated by lozenge.";
RL Neuron 25:69-78(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH DA, AND TISSUE SPECIFICITY.
RX PubMed=10707972; DOI=10.1016/s0896-6273(00)80871-5;
RA Huang M.L., Hsu C.H., Chien C.T.;
RT "The proneural gene amos promotes multiple dendritic neuron formation in
RT the Drosophila peripheral nervous system.";
RL Neuron 25:57-67(2000).
CC -!- FUNCTION: Transcription factor involved in early neurogenesis;
CC sensillum basiconica formation and maybe sensillum trichodea
CC development. Promotes multiple dendritic (MD) neuron formation.
CC Required for olfactory sensilla; regulated by lozenge (lz).
CC {ECO:0000269|PubMed:10707972, ECO:0000269|PubMed:10707973}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Interacts with Daughterless (da).
CC {ECO:0000269|PubMed:10707972}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: During embryonic development, expression is seen in
CC a small cluster of ectodermal cells during stage 10 which becomes
CC restricted to 1 cell by stage 11. Expression is lost from this cell in
CC the thorax and then the abdomen. Later expression is restricted to
CC sensory organ precursors. Very transient expression was detected in
CC distal leg disks at approximately 0-4 hours after puparium formation
CC (APF), correlating with the anlage of the innervated tarsal claw.
CC {ECO:0000269|PubMed:10707972, ECO:0000269|PubMed:10707973}.
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DR EMBL; AF166113; AAD45410.1; -; mRNA.
DR EMBL; AE014134; AAF53678.1; -; Genomic_DNA.
DR EMBL; BT028832; ABI34213.2; -; mRNA.
DR EMBL; BT028865; ABI34246.2; -; mRNA.
DR RefSeq; NP_477446.1; NM_058098.3.
DR AlphaFoldDB; Q9Y0A7; -.
DR SMR; Q9Y0A7; -.
DR BioGRID; 61105; 21.
DR DIP; DIP-18188N; -.
DR IntAct; Q9Y0A7; 4.
DR STRING; 7227.FBpp0080631; -.
DR PaxDb; Q9Y0A7; -.
DR DNASU; 35110; -.
DR EnsemblMetazoa; FBtr0081081; FBpp0080631; FBgn0003270.
DR GeneID; 35110; -.
DR KEGG; dme:Dmel_CG10393; -.
DR CTD; 35110; -.
DR FlyBase; FBgn0003270; amos.
DR VEuPathDB; VectorBase:FBgn0003270; -.
DR eggNOG; KOG4395; Eukaryota.
DR GeneTree; ENSGT00940000168200; -.
DR HOGENOM; CLU_1379447_0_0_1; -.
DR InParanoid; Q9Y0A7; -.
DR OMA; SLGHDRR; -.
DR OrthoDB; 1373473at2759; -.
DR PhylomeDB; Q9Y0A7; -.
DR SignaLink; Q9Y0A7; -.
DR BioGRID-ORCS; 35110; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35110; -.
DR PRO; PR:Q9Y0A7; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0003270; Expressed in antennal primordium (Drosophila) and 20 other tissues.
DR Genevisible; Q9Y0A7; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0008052; P:sensory organ boundary specification; IMP:FlyBase.
DR GO; GO:0007423; P:sensory organ development; IMP:FlyBase.
DR GO; GO:0016360; P:sensory organ precursor cell fate determination; IDA:FlyBase.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Neurogenesis; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..198
FT /note="Basic helix-loop-helix transcription factor amos"
FT /id="PRO_0000127432"
FT DOMAIN 138..190
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 76..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 25
FT /note="S -> G (in Ref. 1; AAD45410)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 198 AA; 22569 MW; F1D22AF2BA1C670B CRC64;
MLTNNELMEQ FYFPDEAPAI PEFLSNDTFQ QLEQLMYQQE FSTSDSQSDG ANSCSLEMYY
DTPSVLELEH MLNAQEQQQH HLQANPLGKN QGRSPRYWNK QQRSKPYDKL STSMSSSTSS
ASSSSSSSAG FGGEVLKKRR LAANARERRR MNSLNDAFDK LRDVVPSLGH DRRLSKYETL
QMAQAYIGDL VTLLSRDY
