ID   GLYCO_VHSV0             Reviewed;         507 AA.
AC   P27662;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   29-SEP-2021, entry version 81.
DE   RecName: Full=Spike glycoprotein;
DE   Flags: Precursor;
GN   Name=G;
OS   Viral hemorrhagic septicemia virus (strain 07-71) (VHSV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Rhabdoviridae; Gammarhabdovirinae;
OC   Novirhabdovirus.
OX   NCBI_TaxID=11288;
OH   NCBI_TaxID=59291; Coregonus lavaretus (Common whitefish) (Salmo lavaretus).
OH   NCBI_TaxID=8010; Esox lucius (Northern pike).
OH   NCBI_TaxID=8019; Oncorhynchus kisutch (Coho salmon) (Salmo kisutch).
OH   NCBI_TaxID=8022; Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OH   NCBI_TaxID=74940; Oncorhynchus tshawytscha (Chinook salmon) (Salmo tshawytscha).
OH   NCBI_TaxID=8032; Salmo trutta (Brown trout).
OH   NCBI_TaxID=8040; Salvelinus namaycush (Lake trout) (Salmo namaycush).
OH   NCBI_TaxID=36185; Thymallus thymallus (Grayling) (Salmo thymallus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1954257; DOI=10.1016/0167-4781(91)90200-6;
RA   Thiry M., Lecoq-Xhonneux F., Dheur I., Renard A., de Kinkelin P.;
RT   "Sequence of a cDNA carrying the glycoprotein gene and part of the matrix
RT   protein M2 gene of viral haemorrhagic scepticaemia virus, a fish
RT   rhabdovirus.";
RL   Biochim. Biophys. Acta 1090:345-347(1991).
CC   -!- FUNCTION: This protein forms spikes on the surface of the virion. It is
CC       responsible both for the binding of the virus to susceptible host cells
CC       and for inducing the uptake of the virus by the cell. The interaction
CC       between the internal components of the virion and the portion of the
CC       glycoprotein exposed on the cytoplasmic face of the plasma membrane
CC       probably directs envelopment and virus budding.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type I
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the novirhabdovirus glycoprotein family.
CC       {ECO:0000305}.
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DR   EMBL; X59148; CAA41859.1; -; Genomic_RNA.
DR   PIR; S15476; S15476.
DR   ABCD; P27662; 3 sequenced antibodies.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR001903; Rhabd_glycop.
DR   InterPro; IPR002417; Spike_prot.
DR   Pfam; PF00974; Rhabdo_glycop; 1.
DR   PRINTS; PR00796; SPIKEPROTEIN.
PE   3: Inferred from homology;
KW   Glycoprotein; Host-virus interaction; Membrane; Signal; Transmembrane;
KW   Transmembrane helix; Viral attachment to host cell; Viral envelope protein;
KW   Virion; Virus entry into host cell.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..507
FT                   /note="Spike glycoprotein"
FT                   /id="PRO_0000041001"
FT   TOPO_DOM        21..461
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        462..482
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        483..507
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        30
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        378
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        389
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        438
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        459
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   507 AA;  57068 MW;  6000448C6F5F1292 CRC64;
     MEWNTFFLVI LIIIIKSTTP QITQRPPVEN ISTYHADWDT PLYTHPSNCR DDSFVPIRPA
     QLRCPHEFED INKGLVSVPT RIIHLPLSVT SVSAVASGHY LHRVTYRVTC STSFFGGQTI
     EKTILEAKLS RQEATDEASK DHEYPFFPEP SCIWMKNNVH KDITHYYKTP KTVSVDLYSR
     KFLNPDFIEG VCTTSPCQTH WQGVYWVGAT PKAHCPTSET LEGHLFTRTH DHRVVKAIVA
     GHHPWGLTMA CTVTFCGTEW IKTDLGDLIQ VTGPGGTRKL TPNKCVNTDI QMRGATDDFS
     YLNHLITNMA QRTECLDAHS DITASGKVSS FLLSKFRPSH PGPGKAHYLL DGQIMRGDCD
     YEAVVSINYN RAQYKTMNNT WKSWKRVDNN TDGYDGMIFG DKLIIPDIEK YQSVYDSGML
     VQRNLVEVPH LSIVFVSNTS DLSTNHIHTN LIPSDWSFNW SLWPSLSGMG VVGGAFLLLV
     LCCCCKASPP IPNYGIPMQQ FSRSQTV