GLYC_CAEBR
ID GLYC_CAEBR Reviewed; 511 AA.
AC Q60V73; A8XW58;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 3.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Serine hydroxymethyltransferase;
DE Short=SHMT;
DE EC=2.1.2.1;
DE AltName: Full=Glycine hydroxymethyltransferase;
DE AltName: Full=Maternal effect lethal protein 32;
DE AltName: Full=Serine methylase;
GN Name=mel-32 {ECO:0000250|UniProtKB:P50432}; ORFNames=CBG19673;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
RN [2] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=10216251; DOI=10.1016/s0378-1119(99)00076-1;
RA Vatcher G.P., Barbazuk W.B., O'Neil N.J., Marra M.A., Ha T., Baillie D.L.;
RT "Identification and characterization of a serine hydroxymethyltransferase
RT isoform in Caenorhabditis briggsae.";
RL Gene 230:137-144(1999).
CC -!- FUNCTION: Interconversion of serine and glycine. {ECO:0000250,
CC ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =
CC (6S)-5,6,7,8-tetrahydrofolate + L-serine; Xref=Rhea:RHEA:15481,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15636, ChEBI:CHEBI:33384,
CC ChEBI:CHEBI:57305, ChEBI:CHEBI:57453; EC=2.1.2.1;
CC Evidence={ECO:0000305};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250|UniProtKB:P35623};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P35623}.
CC -!- SIMILARITY: Belongs to the SHMT family. {ECO:0000255}.
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DR EMBL; HE600934; CAP36877.2; -; Genomic_DNA.
DR AlphaFoldDB; Q60V73; -.
DR SMR; Q60V73; -.
DR STRING; 6238.CBG19673; -.
DR PRIDE; Q60V73; -.
DR EnsemblMetazoa; CBG19673.1; CBG19673.1; WBGene00038848.
DR WormBase; CBG19673; CBP36659; WBGene00038848; Cbr-mel-32.
DR eggNOG; KOG2467; Eukaryota.
DR HOGENOM; CLU_022477_0_1_1; -.
DR InParanoid; Q60V73; -.
DR OMA; SHPAGLI; -.
DR OrthoDB; 372408at2759; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000008549; Chromosome III.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004372; F:glycine hydroxymethyltransferase activity; IBA:GO_Central.
DR GO; GO:0048027; F:mRNA 5'-UTR binding; IBA:GO_Central.
DR GO; GO:0000900; F:mRNA regulatory element binding translation repressor activity; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; IBA:GO_Central.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IBA:GO_Central.
DR GO; GO:0070905; F:serine binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IBA:GO_Central.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IEA:EnsemblMetazoa.
DR GO; GO:0046655; P:folic acid metabolic process; IBA:GO_Central.
DR GO; GO:0019264; P:glycine biosynthetic process from serine; IBA:GO_Central.
DR GO; GO:0006565; P:L-serine catabolic process; IBA:GO_Central.
DR GO; GO:0006730; P:one-carbon metabolic process; IBA:GO_Central.
DR GO; GO:0051289; P:protein homotetramerization; IBA:GO_Central.
DR GO; GO:0009113; P:purine nucleobase biosynthetic process; IBA:GO_Central.
DR GO; GO:0009069; P:serine family amino acid metabolic process; TAS:UniProtKB.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IBA:GO_Central.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IBA:GO_Central.
DR CDD; cd00378; SHMT; 1.
DR Gene3D; 3.40.640.10; -; 1.
DR Gene3D; 3.90.1150.10; -; 1.
DR HAMAP; MF_00051; SHMT; 1.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR001085; Ser_HO-MeTrfase.
DR InterPro; IPR019798; Ser_HO-MeTrfase_PLP_BS.
DR InterPro; IPR039429; SHMT-like_dom.
DR PANTHER; PTHR11680; PTHR11680; 1.
DR Pfam; PF00464; SHMT; 1.
DR PIRSF; PIRSF000412; SHMT; 1.
DR SUPFAM; SSF53383; SSF53383; 1.
DR PROSITE; PS00096; SHMT; 1.
PE 3: Inferred from homology;
KW One-carbon metabolism; Pyridoxal phosphate; Reference proteome;
KW Transferase.
FT CHAIN 1..511
FT /note="Serine hydroxymethyltransferase"
FT /id="PRO_0000282955"
FT MOD_RES 287
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 511 AA; 56213 MW; 715AA585E4D2AECF CRC64;
MFARAVSRKT MTGLLAGSRV LLASQFTMAD RQVHTPLEPV QRQKYANNEN LLKDHVEKID
PEVFNIMKNE KSRQRRGLEL IASENFTSKA VMDALGSAMC NKYSEGYPGA RYYGGNEFID
QMEILCQKRA LEVFGLDPAK WGVNVQSLSG SPANFAVYTA LVGANGRIMG LDLPDGGHLT
HGFFTPARKV SATSEFFQSM PYKVDAQSGL IDYDKLEENA MLFRPKVLIA GVSCYARHLD
YERFRKIATK AGAYLMSDMA HISGLVAAGL IPSPFEYSDV VTTTTHKSLR GPRGAMIFYR
KGVRSVNAKG VETLYDLEEK INSAVFPGLQ GGPHNHTIAG IAVALKQCLS EDFVQYGEQI
LKNAKTLAER LKKHGYSLAT GGTDNHLLLV DLRPIGVEGA RAEHILDLAH IACNKNTCPG
DVSALRPGGI RLGTPALTSR GFKEQDFEKV GDFIHEGVQI AKKYNAEAGK TLKDFKAFTA
TNEQFKQEVA ELAKRVEEFS GKFEIPGNDL F
