ID   GLYC_PONAB              Reviewed;         483 AA.
AC   Q5RFK5;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Serine hydroxymethyltransferase, cytosolic;
DE            Short=SHMT;
DE            EC=2.1.2.1 {ECO:0000250|UniProtKB:P34896};
DE   AltName: Full=Glycine hydroxymethyltransferase;
DE   AltName: Full=Serine methylase;
GN   Name=SHMT1;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Interconversion of serine and glycine.
CC       {ECO:0000250|UniProtKB:P34896}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =
CC         (6S)-5,6,7,8-tetrahydrofolate + L-serine; Xref=Rhea:RHEA:15481,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15636, ChEBI:CHEBI:33384,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:57453; EC=2.1.2.1;
CC         Evidence={ECO:0000250|UniProtKB:P34896};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250|UniProtKB:P34896};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000250|UniProtKB:P34896}.
CC   -!- SUBUNIT: Homotetramer. Identified in complex with ABRAXAS2 and the
CC       other subunits of the BRISC complex, at least composed of ABRAXAS2,
CC       BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. {ECO:0000250|UniProtKB:P34896}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: In eukaryotes there are two forms of the enzymes: a
CC       cytosolic one and a mitochondrial one.
CC   -!- SIMILARITY: Belongs to the SHMT family. {ECO:0000305}.
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DR   EMBL; CR857151; CAH89452.1; -; mRNA.
DR   RefSeq; NP_001124622.1; NM_001131150.1.
DR   AlphaFoldDB; Q5RFK5; -.
DR   SMR; Q5RFK5; -.
DR   STRING; 9601.ENSPPYP00000009057; -.
DR   GeneID; 100171460; -.
DR   KEGG; pon:100171460; -.
DR   CTD; 6470; -.
DR   eggNOG; KOG2467; Eukaryota.
DR   InParanoid; Q5RFK5; -.
DR   OrthoDB; 372408at2759; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0004372; F:glycine hydroxymethyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; ISS:UniProtKB.
DR   GO; GO:0019264; P:glycine biosynthetic process from serine; IEA:InterPro.
DR   GO; GO:0006544; P:glycine metabolic process; ISS:UniProtKB.
DR   GO; GO:0006563; P:L-serine metabolic process; ISS:UniProtKB.
DR   GO; GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; ISS:UniProtKB.
DR   CDD; cd00378; SHMT; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   HAMAP; MF_00051; SHMT; 1.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   InterPro; IPR001085; Ser_HO-MeTrfase.
DR   InterPro; IPR019798; Ser_HO-MeTrfase_PLP_BS.
DR   InterPro; IPR039429; SHMT-like_dom.
DR   PANTHER; PTHR11680; PTHR11680; 1.
DR   Pfam; PF00464; SHMT; 1.
DR   PIRSF; PIRSF000412; SHMT; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   PROSITE; PS00096; SHMT; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; One-carbon metabolism; Pyridoxal phosphate; Reference proteome;
KW   Transferase.
FT   CHAIN           1..483
FT                   /note="Serine hydroxymethyltransferase, cytosolic"
FT                   /id="PRO_0000291856"
FT   MOD_RES         257
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250|UniProtKB:P34896"
SQ   SEQUENCE   483 AA;  53002 MW;  003940BB312E3B56 CRC64;
     MTMPVNGAHK DADLWSSHDK MLAQPLKDSD VEVYNIIKKE SNRQRVGLEL FASENFASQA
     VLEALGSCLN NKYSEGYPGQ RYYGGTEFID ELETLCQKRA LQAYKLDPQC WGVNVQPYSG
     SPANFAVYTA LVEPHGRIMG LDLPDGGHLT HGFMTGKKKI SATSIFFESM PYKVNPDTGY
     INYDQLEENA RLFHPKLIIA GTSCYSRNLD YARLRKIADE NGAYLMADMA HISGLVAAGV
     VPSPFEHCHV VTTTTHKTLR GCRAGMIFYR KGVQSVDPKT GKEILYNLES LINSAVFPGL
     QGGPHNHAIA GVAVALKQAM TLEFKVYQHQ VVANCRALSE ALTELGYKIV TGGSDNHLIL
     VDLRSKGTDG GRAEKVLEAC SIACNKNTCP GDRSALRPSG LRLGTPALTS RGLLEKDFQK
     VAHFIHRGIE LTLQIQSDTG VRATLKEFKE RLAGDKYQGA VQALREKVES FASLFPLPGL
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