ID   AMP1_ACRMI              Reviewed;         117 AA.
AC   P0DUG2;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   07-APR-2021, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Antimicrobial peptide AmAMP1 {ECO:0000303|PubMed:32937163};
DE   Flags: Precursor;
OS   Acropora millepora (Staghorn coral) (Heteropora millepora).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Scleractinia;
OC   Astrocoeniina; Acroporidae; Acropora.
OX   NCBI_TaxID=45264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SYNTHESIS OF 73-117 AND
RP   74-117, BIOPHYSICOCHEMICAL PROPERTIES, AND DEVELOPMENTAL STAGE.
RX   PubMed=32937163; DOI=10.1016/j.dci.2020.103866;
RA   Mason B., Cooke I., Moya A., Augustin R., Lin M.F., Satoh N., Bosch T.C.G.,
RA   Bourne D.G., Hayward D.C., Andrade N., Foret S., Ying H., Ball E.E.,
RA   Miller D.J.;
RT   "AmAMP1 from Acropora millepora and damicornin define a family of coral-
RT   specific antimicrobial peptides related to the Shk toxins of sea
RT   anemones.";
RL   Dev. Comp. Immunol. 114:103866-103866(2021).
CC   -!- FUNCTION: Coral peptide that probably acts as an antimicrobial peptide
CC       in the surface mucous layer of planula larvae and likely also in
CC       adults. Shows moderate to high activity against some Gram-negative and
CC       Gram-positive bacteria (tested on E.coli, B.megaterium, S.aureus,
CC       E.aesturaii, B.algicola, Acinetobacter spec.). Does not show
CC       antibacterial activity against the coral pathogen V.coralliilyticus.
CC       {ECO:0000269|PubMed:32937163}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Shows more potent antibacterial activity at pH 6.2 (approximate coral
CC         mucus pH) than at pH 8.0 (approximate pH of seawater).
CC         {ECO:0000269|PubMed:32937163};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:32937163}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at late stages of coral development
CC       (late planula, polyps, and adults), in ectodermal cells.
CC       {ECO:0000269|PubMed:32937163}.
CC   -!- SIMILARITY: Belongs to the coral AMP family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=National Center for Biotechnology Information
CC       (NCBI);
CC       URL="https://www.ncbi.nlm.nih.gov/nuccore/NW_021126416.1?report=genbank&from=263881&to=267654";
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DR   AlphaFoldDB; P0DUG2; -.
DR   SMR; P0DUG2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   PROPEP          26..73
FT                   /evidence="ECO:0000305|PubMed:32937163"
FT                   /id="PRO_0000452122"
FT   CHAIN           74..117
FT                   /note="Antimicrobial peptide AmAMP1"
FT                   /evidence="ECO:0000305|PubMed:32937163"
FT                   /id="PRO_0000452123"
FT   DISULFID        76..115
FT                   /evidence="ECO:0000250|UniProtKB:P29187"
FT   DISULFID        85..108
FT                   /evidence="ECO:0000250|UniProtKB:P29187"
FT   DISULFID        94..112
FT                   /evidence="ECO:0000250|UniProtKB:P29187"
SQ   SEQUENCE   117 AA;  13386 MW;  8F484752415E2BC2 CRC64;
     MPSIRVLFVL LAVILLFMEV KMTSAASIVK DVDEDETLEN EDGEAMENSW PWHGVEDTSD
     YSDLSDLANS EKRGTCIDLG SRLYCKLIRR RGMCRSRSHR ARIAMMRCER SCGRCHL