GLYK_ARATH
ID GLYK_ARATH Reviewed; 456 AA.
AC Q944I4; A8MQE0; Q8LFL2; Q9C5L9; Q9C967;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=D-glycerate 3-kinase, chloroplastic {ECO:0000305};
DE Short=AtGLYK {ECO:0000303|PubMed:15980259};
DE EC=2.7.1.31 {ECO:0000269|PubMed:15980259};
DE Flags: Precursor;
GN Name=GLYK {ECO:0000303|PubMed:15980259};
GN OrderedLocusNames=At1g80380 {ECO:0000312|Araport:AT1G80380};
GN ORFNames=F5I6.13 {ECO:0000312|EMBL:AAG52441.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP CATALYTIC ACTIVITY, IDENTIFICATION BY MASS SPECTROMETRY, AND FUNCTION.
RX PubMed=15980259; DOI=10.1105/tpc.105.033993;
RA Boldt R., Edner C., Kolukisaoglu U., Hagemann M., Weckwerth W.,
RA Wienkoop S., Morgenthal K., Bauwe H.;
RT "D-glycerate 3-kinase, the last unknown enzyme in the photorespiratory
RT cycle in Arabidopsis, belongs to a novel kinase family.";
RL Plant Cell 17:2413-2420(2005).
RN [6]
RP FUNCTION (ISOFORM 3), SUBCELLULAR LOCATION (ISOFORMS 1 AND 3), ALTERNATIVE
RP SPLICING, AND INDUCTION.
RX PubMed=29129375; DOI=10.1016/j.cell.2017.10.018;
RA Ushijima T., Hanada K., Gotoh E., Yamori W., Kodama Y., Tanaka H.,
RA Kusano M., Fukushima A., Tokizawa M., Yamamoto Y.Y., Tada Y., Suzuki Y.,
RA Matsushita T.;
RT "Light controls protein localization through phytochrome-mediated
RT alternative promoter selection.";
RL Cell 0:0-0(2017).
CC -!- FUNCTION: Catalyzes the concluding reaction of the photorespiratory C2
CC cycle, an indispensable ancillary metabolic pathway to the
CC photosynthetic C3 cycle that enables land plants to grow in an oxygen-
CC containing atmosphere. {ECO:0000269|PubMed:15980259}.
CC -!- FUNCTION: [Isoform 3]: Cytoplasmic D-glycerate 3-kinase that
CC constitutes a photorespiratory bypass that alleviates fluctuating
CC light-induced photoinhibition (PubMed:29129375).
CC {ECO:0000269|PubMed:29129375}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-glycerate + ATP = (2R)-3-phosphoglycerate + ADP + H(+);
CC Xref=Rhea:RHEA:23516, ChEBI:CHEBI:15378, ChEBI:CHEBI:16659,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58272, ChEBI:CHEBI:456216;
CC EC=2.7.1.31; Evidence={ECO:0000269|PubMed:15980259};
CC -!- PATHWAY: Photosynthesis; photorespiration; 3-phospho-D-glycerate from
CC glycine: step 4/4. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Plastid, chloroplast
CC {ECO:0000269|PubMed:29129375}. Note=Expressed in the light.
CC {ECO:0000269|PubMed:29129375}.
CC -!- SUBCELLULAR LOCATION: [Isoform 3]: Cytoplasm
CC {ECO:0000269|PubMed:29129375}. Note=Expressed in the dark.
CC {ECO:0000269|PubMed:29129375}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q944I4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q944I4-2; Sequence=VSP_015208;
CC Name=3;
CC IsoId=Q944I4-3; Sequence=VSP_059299;
CC -!- INDUCTION: Isoform 1: Expressed in the light (PubMed:29129375). Isoform
CC 3: Expressed in the dark (PubMed:29129375).
CC {ECO:0000269|PubMed:29129375}.
CC -!- MISCELLANEOUS: [Isoform 1]: Expressed in the light.
CC {ECO:0000269|PubMed:29129375}.
CC -!- MISCELLANEOUS: [Isoform 3]: Expressed in the dark.
CC {ECO:0000269|PubMed:29129375}.
CC -!- SIMILARITY: Belongs to the GLYK kinase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52441.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC018848; AAG52441.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE36393.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36394.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36395.1; -; Genomic_DNA.
DR EMBL; AF360156; AAK25866.1; -; mRNA.
DR EMBL; AF428401; AAL16169.1; -; mRNA.
DR EMBL; AY056310; AAL07159.1; -; mRNA.
DR EMBL; AY084779; AAM61346.1; -; mRNA.
DR PIR; E96835; E96835.
DR RefSeq; NP_001077855.1; NM_001084386.2. [Q944I4-3]
DR RefSeq; NP_565237.1; NM_106687.2. [Q944I4-2]
DR RefSeq; NP_849912.1; NM_179581.3. [Q944I4-1]
DR AlphaFoldDB; Q944I4; -.
DR SMR; Q944I4; -.
DR STRING; 3702.AT1G80380.2; -.
DR iPTMnet; Q944I4; -.
DR PaxDb; Q944I4; -.
DR PRIDE; Q944I4; -.
DR EnsemblPlants; AT1G80380.1; AT1G80380.1; AT1G80380. [Q944I4-2]
DR EnsemblPlants; AT1G80380.2; AT1G80380.2; AT1G80380. [Q944I4-1]
DR EnsemblPlants; AT1G80380.3; AT1G80380.3; AT1G80380. [Q944I4-3]
DR GeneID; 844378; -.
DR Gramene; AT1G80380.1; AT1G80380.1; AT1G80380. [Q944I4-2]
DR Gramene; AT1G80380.2; AT1G80380.2; AT1G80380. [Q944I4-1]
DR Gramene; AT1G80380.3; AT1G80380.3; AT1G80380. [Q944I4-3]
DR KEGG; ath:AT1G80380; -.
DR Araport; AT1G80380; -.
DR TAIR; locus:2034260; AT1G80380.
DR eggNOG; KOG2878; Eukaryota.
DR InParanoid; Q944I4; -.
DR PhylomeDB; Q944I4; -.
DR BioCyc; ARA:AT1G80380-MON; -.
DR BioCyc; MetaCyc:AT1G80380-MON; -.
DR BRENDA; 2.7.1.31; 399.
DR UniPathway; UPA00951; UER00917.
DR PRO; PR:Q944I4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q944I4; baseline and differential.
DR Genevisible; Q944I4; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008887; F:glycerate kinase activity; IDA:TAIR.
DR GO; GO:0009854; P:oxidative photosynthetic carbon pathway; IEA:UniProtKB-UniPathway.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0009853; P:photorespiration; IMP:TAIR.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR006083; PRK/URK.
DR Pfam; PF00485; PRK; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Chloroplast; Cytoplasm;
KW Glycolate pathway; Kinase; Nucleotide-binding; Photorespiration; Plastid;
KW Reference proteome; Transferase; Transit peptide.
FT TRANSIT 1..63
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 64..456
FT /note="D-glycerate 3-kinase, chloroplastic"
FT /id="PRO_0000012282"
FT BINDING 215..222
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..92
FT /note="Missing (in isoform 3)"
FT /id="VSP_059299"
FT VAR_SEQ 206..299
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_015208"
FT CONFLICT 188
FT /note="W -> C (in Ref. 4; AAM61346)"
FT /evidence="ECO:0000305"
FT CONFLICT 239
FT /note="S -> P (in Ref. 3; AAL16169)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 456 AA; 51018 MW; 5961E0FC6E2220FB CRC64;
MAVAISGSSL ISSTLQHYNN RIYIRDYPIP CHSSNPICNS FNFKRRSFSP SSPKFNDHVV
NPSSSYLSSK LSPIRTHSSF AACGCSWIQD NSMVHDYATT TNGTSKRCSA LPTTNTVDVS
SVSDLFEFIC SGPLVNKIGI TPQRVGQSID KWLLYGSQLC RLFQLNELKL TIPQKARLYH
YYIPVFIWCE DQIALHNSKF KDGDDVPPLV IGFSAPQGCG KTTLVFALDY LFKTTKKKSA
TISVDDFYLT AEGQAELRKK NPGNALLEYR GNAGSHDLKL SVETLEALSK LTKEGLKMKV
PRYNKSAYSG RGDRADSSTW PEVEGPLSVI LFEGWMLGFK PLPADVVKAV DPQLEVVNKN
LEAYYDAWDK YIDAWVVIKI QDPSYVYRWR LQAEIAMRQD GQAGMSDEEV NDFVSRYLPA
YKAYLPTLYA EGPSGSDPDR VLAIDIDEER NPILAN
