ID   GLYM_SOLTU              Reviewed;         518 AA.
AC   P50433;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Serine hydroxymethyltransferase, mitochondrial;
DE            Short=SHMT;
DE            EC=2.1.2.1;
DE   AltName: Full=Glycine hydroxymethyltransferase;
DE   AltName: Full=Serine methylase;
DE   Flags: Precursor;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   PubMed=7870818; DOI=10.1104/pp.107.1.271;
RA   Kopriva S., Bauwe H.;
RT   "Serine hydroxymethyltransferase from Solanum tuberosum.";
RL   Plant Physiol. 107:271-272(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. DM1-3 516 R44;
RX   PubMed=21743474; DOI=10.1038/nature10158;
RG   The Potato Genome Sequencing Consortium;
RT   "Genome sequence and analysis of the tuber crop potato.";
RL   Nature 475:189-195(2011).
CC   -!- FUNCTION: Catalyzes the interconversion of serine and glycine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O =
CC         (6S)-5,6,7,8-tetrahydrofolate + L-serine; Xref=Rhea:RHEA:15481,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15636, ChEBI:CHEBI:33384,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:57453; EC=2.1.2.1;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the SHMT family. {ECO:0000305}.
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DR   EMBL; Z25863; CAA81082.1; -; mRNA.
DR   PIR; S40218; S40218.
DR   RefSeq; NP_001275486.1; NM_001288557.1.
DR   RefSeq; XP_015169067.1; XM_015313581.1.
DR   AlphaFoldDB; P50433; -.
DR   SMR; P50433; -.
DR   IntAct; P50433; 1.
DR   STRING; 4113.PGSC0003DMT400078666; -.
DR   PRIDE; P50433; -.
DR   EnsemblPlants; PGSC0003DMT400078666; PGSC0003DMT400078666; PGSC0003DMG400030612.
DR   GeneID; 102591486; -.
DR   Gramene; PGSC0003DMT400078666; PGSC0003DMT400078666; PGSC0003DMG400030612.
DR   KEGG; sot:102591486; -.
DR   eggNOG; KOG2467; Eukaryota.
DR   HOGENOM; CLU_022477_0_1_1; -.
DR   InParanoid; P50433; -.
DR   OMA; SHPAGLI; -.
DR   OrthoDB; 372408at2759; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004372; F:glycine hydroxymethyltransferase activity; IBA:GO_Central.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IBA:GO_Central.
DR   GO; GO:0070905; F:serine binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IBA:GO_Central.
DR   GO; GO:0046655; P:folic acid metabolic process; IBA:GO_Central.
DR   GO; GO:0019264; P:glycine biosynthetic process from serine; IBA:GO_Central.
DR   GO; GO:0006565; P:L-serine catabolic process; IBA:GO_Central.
DR   GO; GO:0006730; P:one-carbon metabolic process; IBA:GO_Central.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IBA:GO_Central.
DR   CDD; cd00378; SHMT; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   HAMAP; MF_00051; SHMT; 1.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   InterPro; IPR001085; Ser_HO-MeTrfase.
DR   InterPro; IPR019798; Ser_HO-MeTrfase_PLP_BS.
DR   InterPro; IPR039429; SHMT-like_dom.
DR   PANTHER; PTHR11680; PTHR11680; 1.
DR   Pfam; PF00464; SHMT; 1.
DR   PIRSF; PIRSF000412; SHMT; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   PROSITE; PS00096; SHMT; 1.
PE   2: Evidence at transcript level;
KW   Mitochondrion; One-carbon metabolism; Pyridoxal phosphate;
KW   Reference proteome; Transferase; Transit peptide.
FT   TRANSIT         1..31
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           32..518
FT                   /note="Serine hydroxymethyltransferase, mitochondrial"
FT                   /id="PRO_0000032573"
FT   MOD_RES         287
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   518 AA;  57146 MW;  A8FA0C8A1F67532C CRC64;
     MAMAIALRRL SATVDKPVKS LYNGGSLYYM SSLPNEAVYD KEKSGVAWPK QLNAPLEVVD
     PEIADIIEHE KARQWKGLEL IPSENFTSVS VMQAVGSVMT NKYSEGYPGA RYYGGNEYID
     MAETLCQKRA LEAFRLDPAK WGVNVQPLSG SPANFQVYTA LLKPHERIMA LDLPHGGHLS
     HGYQTDTKKI SAVSIFFETM PYRLDESTGY IDYDQLEKSA TLFRPKLIVA GASAYARLYD
     YDRIRKVCNK QKAILLADMA HISGLVAAGV IPSPFDYADV VTTTTHKSLR GPRGAMIFYR
     KGVKEVNKQG KEVFYDYEDK INQAVFPGLQ GGPHNHTITG LAVALKQATT PEYRAYQEQV
     LSNSSKFAQA LGEKGYELVS GGTDNHLVLV NMKNKGIDGS RVEKVLEAVH IAANKNTVPG
     DVSAMVPGGI RMGTPALTSR GFLEEDFVKV ADFFDAAVKI AVKVKAETQG TKLKDFVATL
     ESSAPIKSEI AKLRHDVEEY AKQFPTIGFE KETMKYKN