ID   AMP1_PAEAL              Reviewed;          12 AA.
AC   B3EWQ6;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 1.
DT   11-DEC-2019, entry version 8.
DE   RecName: Full=Antimicrobial protein AN5-1 {ECO:0000303|PubMed:23508455};
OS   Paenibacillus alvei (Bacillus alvei).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=44250;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND MASS
RP   SPECTROMETRY.
RC   STRAIN=AN5 {ECO:0000269|PubMed:23508455};
RX   PubMed=23508455; DOI=10.1007/s10295-013-1259-5;
RA   Alkotaini B., Anuar N., Kadhum A.A., Sani A.A.;
RT   "Detection of secreted antimicrobial peptides isolated from cell-free
RT   culture supernatant of Paenibacillus alvei AN5.";
RL   J. Ind. Microbiol. Biotechnol. 40:571-579(2013).
CC   -!- FUNCTION: Has bactericidal activity against E.coli ATCC 29522 and
CC       S.aureus. Inhibits growth of S.marcescens and B.cereus ATCC 14579. A
CC       synthetic peptide has antibacterial activity against E.coli ATCC 29522
CC       (MIC=8 ug/ml), S.aureus (MIC=64 ug/ml), S.marcescens (MIC=32 ug/ml),
CC       B.cereus ATCC 14579 (MIC=64 ug/ml), B.subtilis (MIC=32 ug/ml),
CC       L.plantarum ATCC 8014 (MIC=32 ug/ml), B.flexus (MIC=32 ug/ml),
CC       S.enteritidis ATCC 13076 (MIC=4 ug/ml), Enterobacter spp (MIC=32
CC       ug/ml), B.anthracis (MIC=128 ug/ml), B.licheniformis (MIC>128 ug/ml)
CC       and L.lactis ATCC 11454 (MIC>128 ug/ml). {ECO:0000269|PubMed:23508455}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Active between pH 2 and 12. {ECO:0000269|PubMed:23508455};
CC       Temperature dependence:
CC         Active between 37 and 90 degrees Celsius. Activity is reduced at 100
CC         degrees Celsius and absent at 110 degrees Celsius. Activity is
CC         unaffected by storage at -20 degrees Celsius.
CC         {ECO:0000269|PubMed:23508455};
CC   -!- MASS SPECTROMETRY: Mass=1316.734; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:23508455};
CC   -!- MISCELLANEOUS: A synthetic peptide has no antibacterial activity
CC       against S.agalactiae ATCC 12386, S.epidermidis, P.aeruginosa and
CC       methicillin-resistant S.aureus (MRSA). {ECO:0000269|PubMed:23508455}.
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DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing.
FT   PEPTIDE         1..12
FT                   /note="Antimicrobial protein AN5-1"
FT                   /evidence="ECO:0000269|PubMed:23508455"
FT                   /id="PRO_0000419697"
SQ   SEQUENCE   12 AA;  1318 MW;  5DE3A6A3A8B73767 CRC64;
     YSKSLPLSVL NP