GMCH_MALS4
ID GMCH_MALS4 Reviewed; 618 AA.
AC M5EAX2;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2013, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=GMC oxidoreductase family protein Mala s 12 {ECO:0000305};
DE EC=1.-.-.- {ECO:0000305};
DE AltName: Allergen=Mala s 12 {ECO:0000305};
DE Flags: Precursor;
GN ORFNames=MSY001_2108 {ECO:0000312|EMBL:CCU99402.1},
GN MSYG_3072 {ECO:0000312|EMBL:SHO78725.1};
OS Malassezia sympodialis (strain ATCC 42132) (Atopic eczema-associated
OS yeast).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Malasseziomycetes; Malasseziales; Malasseziaceae; Malassezia.
OX NCBI_TaxID=1230383 {ECO:0000312|Proteomes:UP000016925};
RN [1] {ECO:0000312|Proteomes:UP000016925}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 42132 {ECO:0000312|Proteomes:UP000016925};
RX PubMed=23341551; DOI=10.1128/mbio.00572-12;
RA Gioti A., Nystedt B., Li W., Xu J., Andersson A., Averette A.F., Muench K.,
RA Wang X., Kappauf C., Kingsbury J.M., Kraak B., Walker L.A., Johansson H.J.,
RA Holm T., Lehtioe J., Stajich J.E., Mieczkowski P., Kahmann R.,
RA Kennell J.C., Cardenas M.E., Lundeberg J., Saunders C.W., Boekhout T.,
RA Dawson T.L., Munro C.A., de Groot P.W.J., Butler G., Heitman J.,
RA Scheynius A.;
RT "Genomic insights into the atopic eczema-associated skin commensal yeast
RT Malassezia sympodialis.";
RL MBio 4:E572-E572(2013).
RN [2] {ECO:0000312|Proteomes:UP000186303}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC 42132;
RX PubMed=28100699; DOI=10.1093/nar/gkx006;
RA Zhu Y., Engstroem P.G., Tellgren-Roth C., Baudo C.D., Kennell J.C., Sun S.,
RA Billmyre R.B., Schroeder M.S., Andersson A., Holm T., Sigurgeirsson B.,
RA Wu G., Sankaranarayanan S.R., Siddharthan R., Sanyal K., Lundeberg J.,
RA Nystedt B., Boekhout T., Dawson T.L. Jr., Heitman J., Scheynius A.,
RA Lehtioe J.;
RT "Proteogenomics produces comprehensive and highly accurate protein-coding
RT gene annotation in a complete genome assembly of Malassezia sympodialis.";
RL Nucleic Acids Res. 45:2629-2643(2017).
RN [3]
RP SUBCELLULAR LOCATION, INDUCTION, AND ALLERGEN.
RX PubMed=16867055; DOI=10.1111/j.1398-9995.2006.01108.x;
RA Selander C., Zargari A., Moellby R., Rasool O., Scheynius A.;
RT "Higher pH level, corresponding to that on the skin of patients with atopic
RT eczema, stimulates the release of Malassezia sympodialis allergens.";
RL Allergy 61:1002-1008(2006).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|PIRSR:PIRSR000137-2};
CC Note=Binds 1 FAD per subunit. {ECO:0000250|UniProtKB:Q5GMY3};
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q5GMY3}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16867055}.
CC -!- INDUCTION: By alkaline pH. Expressed 12 times higher in tube
CC cultivation grown at pH 6.1 for 2 days compared with cells cultured at
CC pH 5.5. However, at day 4, the levels were equal at both pH values. In
CC the fermentor cultivations, expressed 32 times more at pH 6.1 than at
CC pH 5.0 after 24 h. At 48 h, the expression level was nine times higher
CC at the higher pH. {ECO:0000269|PubMed:16867055}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE. The skin
CC barrier with higher pH level in atopic eczema patients seems to provide
CC an environment that can enhance the release of this allergen from
CC M.sympodialis, which can contribute to the inflammation.
CC {ECO:0000269|PubMed:16867055}.
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC {ECO:0000255|RuleBase:RU003968}.
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DR EMBL; HE999557; CCU99402.1; -; Genomic_DNA.
DR EMBL; LT671824; SHO78725.1; -; Genomic_DNA.
DR RefSeq; XP_018740653.1; XM_018883944.1.
DR AlphaFoldDB; M5EAX2; -.
DR SMR; M5EAX2; -.
DR STRING; 1230383.M5EAX2; -.
DR Allergome; 2530; Mala s 12.
DR EnsemblFungi; CCU99402; CCU99402; MSY001_2108.
DR EnsemblFungi; SHO78725; SHO78725; MSYG_3072.
DR GeneID; 30056993; -.
DR KEGG; msym:MSY001_2108; -.
DR VEuPathDB; FungiDB:MSYG_3072; -.
DR HOGENOM; CLU_002865_6_0_1; -.
DR OMA; DEDWIAW; -.
DR OrthoDB; 798314at2759; -.
DR Proteomes; UP000016925; Unassembled WGS sequence.
DR Proteomes; UP000186303; Chromosome 4.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0016614; F:oxidoreductase activity, acting on CH-OH group of donors; IEA:InterPro.
DR Gene3D; 3.50.50.60; -; 1.
DR Gene3D; 4.10.450.10; -; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR027424; Glucose_Oxidase_domain_2.
DR InterPro; IPR012132; GMC_OxRdtase.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR PANTHER; PTHR11552; PTHR11552; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR PROSITE; PS00623; GMC_OXRED_1; 1.
DR PROSITE; PS00624; GMC_OXRED_2; 1.
PE 1: Evidence at protein level;
KW Allergen; FAD; Flavoprotein; Oxidoreductase; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..618
FT /note="GMC oxidoreductase family protein Mala s 12"
FT /evidence="ECO:0000255"
FT /id="PRO_5015096524"
FT ACT_SITE 556
FT /note="Proton donor"
FT /evidence="ECO:0000255|PIRSR:PIRSR000137-1"
FT ACT_SITE 599
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PIRSR:PIRSR000137-1"
FT BINDING 129
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|PIRSR:PIRSR000137-2"
FT BINDING 280
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|PIRSR:PIRSR000137-2"
SQ SEQUENCE 618 AA; 66277 MW; 063AFC9322F9E324 CRC64;
MKGIVSWAVV SAALVLSATE SLAFANVSSF EKRTTTGNGW DLDGKSYDYV IVGGGTAGLV
LANRLSANQG TTVAVIEAGN SGYDDNDKFV VPDANLYNSA VNTQYDWQFH TSSQKHMNNR
RASWPRGKVL GGSSAVNGLY YVRPSETEVN VWSKLAGGSG RWSWNSLLSG MKKSEHFRGP
VKSVQNQLQI QYNAGSHGSN GPIGTTWPAV TYDPVERFIK TADSMSGAIN NDPYNGNNHG
TYVALSSIDK TNWQRSFSRN GYLDPISKRS NLHVLTGHTV TGIIFDRSGK NAQATGVHYA
ASSNEASHTV HANKEVIISG GAINSPQILQ LSGIGDKNLL NGLGIDVVVD LPGVGENLQD
HVSAGMSFKP KNKKDAGPTS VTGDAKADSY VNSAVSYTSL GKLFNNKDSI LGKIQARAKQ
IADSHNVSPA VKQGQSKAYN ALADTIFPSK VSPVEILGNV MFGSISIQAA LQHPLSRGSI
KITSKDPFAY PKINPNYFAE NLDLVLLREG FKLIREMSQQ SPLKDVIDFE TVPGDKVQTN
EDWENWIRSA AGTEYHPSST CAMLPRGDGG VVDENLKVYG TSNLRVVDAS VTPIAMSCHL
ESVVYGLAEV AADIILGN
