ID   GMCH_MALSM              Reviewed;         618 AA.
AC   Q5GMY3;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 2.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=GMC oxidoreductase family protein Mala s 12.0101 {ECO:0000305};
DE            EC=1.-.-.- {ECO:0000305};
DE   AltName: Allergen=Mala s 12.0101 {ECO:0000305};
DE   Flags: Precursor;
OS   Malassezia sympodialis (Atopic eczema-associated yeast).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Malasseziomycetes; Malasseziales; Malasseziaceae; Malassezia.
OX   NCBI_TaxID=76777 {ECO:0000312|EMBL:CAI43283.4};
RN   [1] {ECO:0000312|EMBL:CAI43283.4}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, SUBUNIT, AND ALLERGEN.
RX   PubMed=17313403; DOI=10.1111/j.1398-9995.2006.01291.x;
RA   Zargari A., Selander C., Rasool O., Ghanem M., Gadda G., Crameri R.,
RA   Scheynius A.;
RT   "Mala s 12 is a major allergen in patients with atopic eczema and has
RT   sequence similarities to the GMC oxidoreductase family.";
RL   Allergy 62:695-703(2007).
CC   -!- FUNCTION: The recombinant protein is not active with choline, glucose,
CC       myo-inositol, methanol, ethanol, 1-pentanol, benzyl alcohol, 2-
CC       phenylethanol, cholesterol or lauryl alcohol as substrates. Binds
CC       membrane lipids including phosphatidylinositol (PtdIns)(4)-phosphate
CC       (P), PtdIns(4,5)P2, phosphatidic acid (PA), and to a lower extent
CC       PtdIns(3,4,5)P3, cardiolipin and 3-sulfogalactosyl ceramide
CC       (sulfatide). {ECO:0000269|PubMed:17313403}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|PIRSR:PIRSR000137-2,
CC         ECO:0000269|PubMed:17313403};
CC       Note=Binds 1 FAD per subunit. {ECO:0000269|PubMed:17313403};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:17313403}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:M5EAX2}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Recombinant protein
CC       binds to IgE in 62% of the 21 atopic eczema patients tested allergic to
CC       M.sympodialis. {ECO:0000269|PubMed:17313403}.
CC   -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC       {ECO:0000255|RuleBase:RU003968}.
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DR   EMBL; AJ871960; CAI43283.4; -; mRNA.
DR   AlphaFoldDB; Q5GMY3; -.
DR   SMR; Q5GMY3; -.
DR   Allergome; 2530; Mala s 12.
DR   Allergome; 3367; Mala s 12.0101.
DR   VEuPathDB; FungiDB:MSYG_3072; -.
DR   OMA; DEDWIAW; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR   GO; GO:0016614; F:oxidoreductase activity, acting on CH-OH group of donors; IEA:InterPro.
DR   Gene3D; 3.50.50.60; -; 1.
DR   Gene3D; 4.10.450.10; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027424; Glucose_Oxidase_domain_2.
DR   InterPro; IPR012132; GMC_OxRdtase.
DR   InterPro; IPR000172; GMC_OxRdtase_N.
DR   InterPro; IPR007867; GMC_OxRtase_C.
DR   PANTHER; PTHR11552; PTHR11552; 1.
DR   Pfam; PF05199; GMC_oxred_C; 1.
DR   Pfam; PF00732; GMC_oxred_N; 1.
DR   PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   PROSITE; PS00623; GMC_OXRED_1; 1.
DR   PROSITE; PS00624; GMC_OXRED_2; 1.
PE   1: Evidence at protein level;
KW   Allergen; FAD; Flavoprotein; Lipid-binding; Oxidoreductase; Secreted;
KW   Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..618
FT                   /note="GMC oxidoreductase family protein Mala s 12.0101"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5004256853"
FT   ACT_SITE        556
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PIRSR:PIRSR000137-1"
FT   ACT_SITE        599
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PIRSR:PIRSR000137-1"
FT   BINDING         129
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|PIRSR:PIRSR000137-2"
FT   BINDING         280
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|PIRSR:PIRSR000137-2"
SQ   SEQUENCE   618 AA;  66277 MW;  063AFC9322F9E324 CRC64;
     MKGIVSWAVV SAALVLSATE SLAFANVSSF EKRTTTGNGW DLDGKSYDYV IVGGGTAGLV
     LANRLSANQG TTVAVIEAGN SGYDDNDKFV VPDANLYNSA VNTQYDWQFH TSSQKHMNNR
     RASWPRGKVL GGSSAVNGLY YVRPSETEVN VWSKLAGGSG RWSWNSLLSG MKKSEHFRGP
     VKSVQNQLQI QYNAGSHGSN GPIGTTWPAV TYDPVERFIK TADSMSGAIN NDPYNGNNHG
     TYVALSSIDK TNWQRSFSRN GYLDPISKRS NLHVLTGHTV TGIIFDRSGK NAQATGVHYA
     ASSNEASHTV HANKEVIISG GAINSPQILQ LSGIGDKNLL NGLGIDVVVD LPGVGENLQD
     HVSAGMSFKP KNKKDAGPTS VTGDAKADSY VNSAVSYTSL GKLFNNKDSI LGKIQARAKQ
     IADSHNVSPA VKQGQSKAYN ALADTIFPSK VSPVEILGNV MFGSISIQAA LQHPLSRGSI
     KITSKDPFAY PKINPNYFAE NLDLVLLREG FKLIREMSQQ SPLKDVIDFE TVPGDKVQTN
     EDWENWIRSA AGTEYHPSST CAMLPRGDGG VVDENLKVYG TSNLRVVDAS VTPIAMSCHL
     ESVVYGLAEV AADIILGN