ID   AMP1_PARCM              Reviewed;          62 AA.
AC   A0A2I8B346;
DT   23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-MAR-2018, sequence version 1.
DT   25-MAY-2022, entry version 10.
DE   RecName: Full=Paralithocin 1 {ECO:0000312|EMBL:AUT12057.1};
DE   AltName: Full=P23 {ECO:0000303|PubMed:29338238};
DE   Flags: Precursor;
OS   Paralithodes camtschaticus (Red king crab).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Anomura;
OC   Paguroidea; Lithodidae; Paralithodes.
OX   NCBI_TaxID=6741 {ECO:0000312|EMBL:AUT12057.1};
RN   [1] {ECO:0000312|EMBL:AUT12057.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-62, FUNCTION, MASS
RP   SPECTROMETRY, AMIDATION AT TYR-61, DISULFIDE BONDS, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   TISSUE=Hemocyte {ECO:0000303|PubMed:29338238};
RX   PubMed=29338238; DOI=10.1021/acs.jnatprod.7b00780;
RA   Moe M.K., Haug T., Sydnes M.O., Sperstad S.V., Li C., Vaagsfjord L.C.,
RA   de la Vega E., Stensvaag K.;
RT   "Paralithocins, Antimicrobial Peptides with Unusual Disulfide Connectivity
RT   from the Red King Crab, Paralithodes camtschaticus.";
RL   J. Nat. Prod. 81:140-150(2018).
CC   -!- FUNCTION: Has weak antibacterial activity, mainly against marine Gram-
CC       positive bacteria like C.maltaromaticum (MIC=200 uM), C.mobile (MIC=100
CC       uM), C.divergens (MIC=200 uM) and C.funditum (MIC=200 uM) but also
CC       against C.glutamicum (MIC=50 uM). Has very little or no activity
CC       against Gram-negative bacteria. {ECO:0000269|PubMed:29338238}.
CC   -!- PTM: The amidated form is probably the active form.
CC       {ECO:0000303|PubMed:29338238}.
CC   -!- MASS SPECTROMETRY: Mass=4075.6248; Mass_error=0.0017;
CC       Method=Electrospray; Note=Amidated.;
CC       Evidence={ECO:0000269|PubMed:29338238};
CC   -!- MASS SPECTROMETRY: Mass=4076.6044; Mass_error=0.0028;
CC       Method=Electrospray; Note=Not amidated.;
CC       Evidence={ECO:0000269|PubMed:29338238};
CC   -!- SIMILARITY: Belongs to the paralithocin family.
CC       {ECO:0000305|PubMed:29338238}.
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DR   EMBL; MF919584; AUT12057.1; -; mRNA.
DR   AlphaFoldDB; A0A2I8B346; -.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:29338238"
FT   PEPTIDE         24..61
FT                   /note="Paralithocin 1"
FT                   /evidence="ECO:0000269|PubMed:29338238"
FT                   /id="PRO_0000444206"
FT   MOD_RES         61
FT                   /note="Tyrosine amide; partial"
FT                   /evidence="ECO:0000269|PubMed:29338238"
FT   DISULFID        29..55
FT                   /evidence="ECO:0000269|PubMed:29338238"
FT   DISULFID        33..51
FT                   /evidence="ECO:0000269|PubMed:29338238"
FT   DISULFID        37..49
FT                   /evidence="ECO:0000269|PubMed:29338238"
FT   DISULFID        42..52
FT                   /evidence="ECO:0000269|PubMed:29338238"
SQ   SEQUENCE   62 AA;  6516 MW;  2ADD8C60FAAAA504 CRC64;
     MGPMKVLLVL LVVMVAAPHI ADAWQQPSCS SICDYSCGKS ACISYSGRCG CCASCRRGPI
     YG