GMCL2_MOUSE
ID GMCL2_MOUSE Reviewed; 498 AA.
AC Q99N64; E9QJS3; Q99N65; Q99N66; Q9DA87;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Germ cell-less protein-like 2;
DE AltName: Full=BTB domain containing 35, family member 1;
DE AltName: Full=Germ cell-less protein-like 1-like;
DE AltName: Full=mGclh;
GN Name=Gmcl2;
GN Synonyms=Btbd35f1 {ECO:0000312|MGI:MGI:1919097}, Gmcl1l, Gmcl1p1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Testis;
RA Aizawa A., Kawakami A., Kondo T.;
RT "Molecular cloning of a novel mouse germ cell-less homologue expressing
RT haploid specifically in mouse testis.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Possible function in spermatogenesis. Probable substrate-
CC specific adapter of an E3 ubiquitin-protein ligase complex which
CC mediates the ubiquitination and subsequent proteasomal degradation of
CC target proteins. {ECO:0000250|UniProtKB:Q8NEA9,
CC ECO:0000250|UniProtKB:Q920G9}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000250|UniProtKB:Q8NEA9}.
CC -!- SUBUNIT: Interacts with CUL3. {ECO:0000250|UniProtKB:Q8NEA9}.
CC -!- SUBCELLULAR LOCATION: Nucleus matrix {ECO:0000250|UniProtKB:Q920G9}.
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DR EMBL; AB055854; BAB40688.1; -; mRNA.
DR EMBL; AB055855; BAB40689.1; -; mRNA.
DR EMBL; AB055856; BAB40690.1; -; mRNA.
DR EMBL; AK006065; BAB24393.1; -; mRNA.
DR EMBL; CT867960; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS53040.1; -.
DR RefSeq; NP_001161809.1; NM_001168337.1.
DR RefSeq; NP_001257596.1; NM_001270667.1.
DR RefSeq; NP_001257598.1; NM_001270669.1.
DR AlphaFoldDB; Q99N64; -.
DR SMR; Q99N64; -.
DR STRING; 10090.ENSMUSP00000136330; -.
DR PaxDb; Q99N64; -.
DR PRIDE; Q99N64; -.
DR Ensembl; ENSMUST00000178747; ENSMUSP00000136615; ENSMUSG00000118409.
DR GeneID; 100040533; -.
DR GeneID; 100861966; -.
DR GeneID; 100862329; -.
DR KEGG; mmu:100040533; -.
DR KEGG; mmu:100861966; -.
DR KEGG; mmu:100862329; -.
DR UCSC; uc012hfq.1; mouse.
DR CTD; 100040533; -.
DR CTD; 100861966; -.
DR CTD; 100862329; -.
DR MGI; MGI:1919097; Btbd35f1.
DR VEuPathDB; HostDB:ENSMUSG00000118409; -.
DR eggNOG; KOG4682; Eukaryota.
DR GeneTree; ENSGT00940000163246; -.
DR HOGENOM; CLU_025961_2_0_1; -.
DR InParanoid; Q99N64; -.
DR OrthoDB; 1111791at2759; -.
DR PhylomeDB; Q99N64; -.
DR TreeFam; TF316048; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 100040533; 0 hits in 19 CRISPR screens.
DR BioGRID-ORCS; 100861966; 1 hit in 28 CRISPR screens.
DR BioGRID-ORCS; 100862329; 2 hits in 25 CRISPR screens.
DR PRO; PR:Q99N64; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q99N64; protein.
DR Bgee; ENSMUSG00000118409; Expressed in spermatid and 2 other tissues.
DR Genevisible; Q99N64; MM.
DR GO; GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0007281; P:germ cell development; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR043380; Gcl-like.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR23231; PTHR23231; 1.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Nucleus; Reference proteome;
KW Spermatogenesis; Ubl conjugation pathway.
FT CHAIN 1..498
FT /note="Germ cell-less protein-like 2"
FT /id="PRO_0000087522"
FT DOMAIN 90..160
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT MOTIF 33..39
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT CONFLICT 5
FT /note="V -> A (in Ref. 2; BAB24393)"
FT /evidence="ECO:0000305"
FT CONFLICT 23
FT /note="A -> G (in Ref. 1; BAB40689)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="G -> V (in Ref. 1; BAB40689)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="R -> N (in Ref. 1; BAB40690 and 2; BAB24393)"
FT /evidence="ECO:0000305"
FT CONFLICT 104
FT /note="H -> Y (in Ref. 2; BAB24393)"
FT /evidence="ECO:0000305"
FT CONFLICT 115
FT /note="A -> T (in Ref. 1; BAB40689)"
FT /evidence="ECO:0000305"
FT CONFLICT 182
FT /note="Q -> K (in Ref. 1; BAB40689)"
FT /evidence="ECO:0000305"
FT CONFLICT 237
FT /note="L -> M (in Ref. 1; BAB40688)"
FT /evidence="ECO:0000305"
FT CONFLICT 267
FT /note="F -> I (in Ref. 1; BAB40688)"
FT /evidence="ECO:0000305"
FT CONFLICT 296
FT /note="V -> I (in Ref. 1; BAB40688)"
FT /evidence="ECO:0000305"
FT CONFLICT 325
FT /note="D -> N (in Ref. 1; BAB40689)"
FT /evidence="ECO:0000305"
FT CONFLICT 464
FT /note="K -> N (in Ref. 1; BAB40689/BAB40688)"
FT /evidence="ECO:0000305"
FT CONFLICT 465
FT /note="E -> Q (in Ref. 1; BAB40689)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 498 AA; 57827 MW; 0343C472F6748FA6 CRC64;
MGLLVSRVLR CRDSSLLEPQ PEAIAGASYI PGSRKRKRNS LEELATSSNV HGPQNQGMYP
HQVLNYIYWK RVKISSNDAY QNLFLDGHDS DIKIRALGRT WCLHKVFLCQ SGYFANILKG
TWRESHHGVI NLIIKNEDID TRSLHFVFGA LYTDADLSIT PLEVPQVLAA ACLLRVDRVI
QQCEGIMKET INRNTVCSYY LAAETYRLKA VKTRCFEWLL CNLMVHPSVA LYKEVDLKLM
YLLALSSDLL VMQKEIDVYT TLKIWMFLYL NPCWNGTMKQ LLQHANNWLS THMAYVDNIS
FLESEEGLIF QPVFKKLRFQ HIICDLTSTT ILEQDRLIPM AWLSPIYKQQ WLTLLRTQEY
GVIGPQVINE QELEECTMRC GTMIPKDGRY TWKWSVGRLG FPLRVTFTRQ CVILRQRCQR
CDGSACHNHI RNVIFRITLV CFDSNKRVTF RKTTGYKILT FEYKEEQIVM KLDSDVLTFP
MCIFCNFLFV NLGNAENK
