ID   AMP1_PINMO              Reviewed;         105 AA.
AC   P83880; Q2M5E6;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 2.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Antimicrobial peptide 1 {ECO:0000303|PubMed:14759906, ECO:0000303|PubMed:18943919};
DE   AltName: Full=10.6 kDa protein {ECO:0000303|PubMed:14759906};
DE   AltName: Full=PMAP1 {ECO:0000303|PubMed:14759906, ECO:0000303|PubMed:18943919};
DE   Flags: Precursor;
OS   Pinus monticola (Western white pine) (Strobus monticola).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC   Pinus subgen. Strobus.
OX   NCBI_TaxID=3345;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAU00106.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP   INDUCTION.
RC   TISSUE=Needle {ECO:0000269|PubMed:18943919};
RX   PubMed=18943919; DOI=10.1094/phyto-96-0164;
RA   Ekramoddoullah A.K.M., Liu J.-J., Zamani A.;
RT   "Cloning and characterization of a putative antifungal peptide gene (Pm-
RT   AMP1) in Pinus monticola.";
RL   Phytopathology 96:164-170(2006).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 27-49.
RC   TISSUE=Bark {ECO:0000269|PubMed:14759906};
RX   PubMed=14759906; DOI=10.1093/treephys/17.10.663;
RA   Davidson J., Ekramoddoullah A.K.M.;
RT   "Analysis of bark proteins in blister rust-resistant and susceptible
RT   western white pine (Pinus monticola).";
RL   Tree Physiol. 17:663-669(1997).
CC   -!- FUNCTION: Antimicrobial peptide. {ECO:0000250|UniProtKB:P80915}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P80915,
CC       ECO:0000250|UniProtKB:P85484}. Secreted, cell wall
CC       {ECO:0000250|UniProtKB:P80915, ECO:0000250|UniProtKB:P85484}.
CC   -!- TISSUE SPECIFICITY: Detected at higher levels in needles and twigs from
CC       canker-resistant seedlings than in needles from canker-susceptible
CC       plants. During summer, detected on cankered, healthy and marginal bark.
CC       During winter, detected at lower levels in cankered bark and bark from
CC       the canker margin than in healthy bark (at protein level).
CC       {ECO:0000269|PubMed:18943919}.
CC   -!- DEVELOPMENTAL STAGE: In summer, present at higher levels in older
CC       needles and twigs than in tissues from the current year. In winter,
CC       detected at high levels in both older and current-year needles and
CC       twigs. {ECO:0000269|PubMed:18943919}.
CC   -!- INDUCTION: By wounding and by methyl jasmonate.
CC       {ECO:0000269|PubMed:18943919}.
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DR   EMBL; AY596276; AAU00106.1; -; mRNA.
DR   AlphaFoldDB; P83880; -.
DR   SMR; P83880; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0045926; P:negative regulation of growth; IEA:InterPro.
DR   Gene3D; 2.60.20.30; -; 1.
DR   InterPro; IPR015791; Antimic/Inh_G_crystallin-like.
DR   InterPro; IPR015201; Antimicrobial_MiAMP1.
DR   InterPro; IPR011024; G_crystallin-like.
DR   Pfam; PF09117; MiAMP1; 1.
DR   SUPFAM; SSF49695; SSF49695; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Cell wall; Direct protein sequencing; Disulfide bond;
KW   Plant defense; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000269|PubMed:14759906"
FT   CHAIN           27..105
FT                   /note="Antimicrobial peptide 1"
FT                   /evidence="ECO:0000269|PubMed:14759906"
FT                   /id="PRO_0000064591"
FT   DISULFID        37..93
FT                   /evidence="ECO:0000250|UniProtKB:P80915"
FT   DISULFID        47..105
FT                   /evidence="ECO:0000250|UniProtKB:P80915"
FT   DISULFID        49..77
FT                   /evidence="ECO:0000250|UniProtKB:P80915"
FT   CONFLICT        32
FT                   /note="W -> Y (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        47..49
FT                   /note="CGC -> KGV (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   105 AA;  11513 MW;  8B9532599DFD88B4 CRC64;
     METKRLAYVM FVLVCLFLAM AQPSQASYFS AWAGPGCNNH NARYSKCGCS NIGHNVHGGY
     EFVYQGQTAA AYNTDNCKGV AQTRFSSSVN QACSNFGWKS VFIQC