ID   GMGT1_CYATE             Reviewed;         435 AA.
AC   Q564G7;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Galactomannan galactosyltransferase 1;
DE            EC=2.4.1.-;
GN   Name=GMGT1;
OS   Cyamopsis tetragonoloba (Guar) (Cluster bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Indigofereae; Cyamopsis.
OX   NCBI_TaxID=3832;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Seed endosperm;
RA   Reid J.S.G., Edwards M.E., Dickson C.A., Gidley M.J., Clark A.H.;
RT   "In vitro galactomannan biosynthesis in tobacco transgenic lines expressing
RT   legume seed galactomannan galactosyltransferases (GMGTs).";
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RX   PubMed=14988472; DOI=10.1104/pp.103.029967;
RA   Edwards M.E., Choo T.-S., Dickson C.A., Scott C., Gidley M.J., Reid J.S.G.;
RT   "The seeds of Lotus japonicus lines transformed with sense, antisense, and
RT   sense/antisense galactomannan galactosyltransferase constructs have
RT   structurally altered galactomannans in their endosperm cell walls.";
RL   Plant Physiol. 134:1153-1162(2004).
CC   -!- FUNCTION: Galactomannan galactosyltransferase (GMGT) involved in
CC       galactomannan biosynthesis in seed endosperm. GMGT specificity is an
CC       important factor regulating the distribution and amount of alpha-1,6-
CC       galactose (Gal) substitution of the beta-1,4-linked mannan backbone.
CC       {ECO:0000269|PubMed:14988472}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000305}.
CC   -!- BIOTECHNOLOGY: Guar gum also called guaran is a galactomannan extracted
CC       from guar seed endosperm. Gums are most commonly used as food additives
CC       to provide stiffness and texture, prevent ice crystal formation,
CC       maintain crispiness, and retain moisture. They have many other nonfood
CC       applications such as dying and printing aids in the textile industry,
CC       thickeners for shampoos and conditioners, binders and hardeners for
CC       paper, rheological facilitators for concrete, and drilling agents for
CC       oil and gas wells.
CC   -!- MISCELLANEOUS: Galactomannan is accumulated only in the endosperm and
CC       constitutes more than 90% of this tissue at maturity. Galactomannan is
CC       made by the combined actions of two enzymes: mannan synthase (ManS),
CC       which makes beta-1,4-linked mannan backbone, and alpha-
CC       galactosyltransferase, which adds galactosyl residues to the mannan
CC       backbone. The mannose/galactose ratio of guar galactomannan is 2. The
CC       degree of galactosyl substitution on the mannan backbone determines the
CC       quality of galactomannan as a gum. A mannose/galactose ratio of 4
CC       provides a gum of higher quality than a ratio of 2.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 34 family.
CC       {ECO:0000305}.
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DR   EMBL; AJ938067; CAI79402.1; -; mRNA.
DR   AlphaFoldDB; Q564G7; -.
DR   SMR; Q564G7; -.
DR   CAZy; GT34; Glycosyltransferase Family 34.
DR   PRIDE; Q564G7; -.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR008630; Glyco_trans_34.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR31311; PTHR31311; 1.
DR   Pfam; PF05637; Glyco_transf_34; 1.
PE   1: Evidence at protein level;
KW   Cell wall biogenesis/degradation; Coiled coil; Glycoprotein;
KW   Glycosyltransferase; Golgi apparatus; Membrane; Signal-anchor; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..435
FT                   /note="Galactomannan galactosyltransferase 1"
FT                   /id="PRO_0000319356"
FT   TOPO_DOM        1..20
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        21..41
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        42..435
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   COILED          321..354
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        230
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        328
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   435 AA;  50956 MW;  F5402E419087B979 CRC64;
     MAKFGSRNKS PKWISNGCCF LLGAFTALLL LWGLCSFIIP IPNTDPKLNS VATSLRSLNF
     PKNPAATLPP NLQHDPPDTT FYDDPETSYT MDKPMKNWDE KRKEWLLHHP SFGAAARDKI
     LLVTGSQPKR CHNPIGDHLL LRFFKNKVDY CRLHNYDIIY NNALLHPKMN SYWAKYPVIR
     AAMMAHPEVE WVWWVDSDAV FTDMEFKLPL KRYKNHNLVV HGWEGLVRLN HSWTGLNAGV
     FLIRNCQWSL EFMDVWVSMG PQTPEYEKWG ERLRETFKDK VLPDSDDQTA LAYLIATDNK
     DTWREKIFLE SEYYFEGYWL EIVKTYENIS ERYDEVERKV EGLRRRHAEK VSEKYGAMRE
     EYLKDNKRRP FITHFTGCQP CNGHHNPAYN ANDCWNGMER ALNFADNQIL RTYGYHRQNL
     LDKSVSPLPF GYPAA