GMH3_SCHPO
ID GMH3_SCHPO Reviewed; 332 AA.
AC Q10359;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Alpha-1,2-galactosyltransferase gmh3;
DE EC=2.4.1.-;
GN Name=gmh3; ORFNames=SPAC22E12.06c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=9839953; DOI=10.1046/j.1432-1327.1998.2570630.x;
RA Yoko-o T., Roy S.K., Jigami Y.;
RT "Differences in in vivo acceptor specificity of two galactosyltransferases,
RT the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe.";
RL Eur. J. Biochem. 257:630-637(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Involved in the galactosylation of the N-linked core
CC oligosaccharide Man(9)GlcNAc(2). {ECO:0000269|PubMed:9839953}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 34 family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAA93893.1; -; Genomic_DNA.
DR PIR; T38163; T38163.
DR RefSeq; NP_594832.1; NM_001020261.2.
DR AlphaFoldDB; Q10359; -.
DR SMR; Q10359; -.
DR BioGRID; 278242; 13.
DR STRING; 4896.SPAC22E12.06c.1; -.
DR CAZy; GT34; Glycosyltransferase Family 34.
DR MaxQB; Q10359; -.
DR PaxDb; Q10359; -.
DR PRIDE; Q10359; -.
DR EnsemblFungi; SPAC22E12.06c.1; SPAC22E12.06c.1:pep; SPAC22E12.06c.
DR PomBase; SPAC22E12.06c; gmh3.
DR VEuPathDB; FungiDB:SPAC22E12.06c; -.
DR eggNOG; KOG4748; Eukaryota.
DR HOGENOM; CLU_021434_2_0_1; -.
DR InParanoid; Q10359; -.
DR OMA; PQAKWIW; -.
DR PhylomeDB; Q10359; -.
DR PRO; PR:Q10359; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000139; C:Golgi membrane; ISS:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031278; F:alpha-1,2-galactosyltransferase activity; IDA:PomBase.
DR GO; GO:0006491; P:N-glycan processing; IMP:PomBase.
DR GO; GO:0006487; P:protein N-linked glycosylation; IDA:PomBase.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR008630; Glyco_trans_34.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR31306; PTHR31306; 1.
DR Pfam; PF05637; Glyco_transf_34; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..332
FT /note="Alpha-1,2-galactosyltransferase gmh3"
FT /id="PRO_0000215168"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..332
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 332 AA; 38054 MW; 998E687D846BCEEE CRC64;
MAVLQWKLSR VIFVLLIIGI SSFLIYFQFG NSFSASPALE RLQDVFSKPS VQEYDYLVPL
DEDMQGLSTT YHQATINEDD PQEPKSHEIV ILLASDGRSS GNMAPETFNQ CIENRINYAK
HHNYGFEYVN VSQMNIPPVW AKMPAIIQTM NKHPHAKWIW WLDQDALILN TELSIQEHIL
SPDVLVEKLM KNEPMISPFS ADLERLTPSS YTVDSARSLG LLISQDLNGL NAGSFFVRRS
PMMALFLDLW GDKSFRENKV ADHEQALLGY FVRYHPEIAA IIGILPQTLI NSYPVGQPEM
GWKEGHLVIH FAGCWVENRC QVLWDEYRER VH
