ID   AMP1_SCYSE              Reviewed;         126 AA.
AC   A1YV58;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Antimicrobial protein 1;
DE   Flags: Precursor;
GN   Name=AP {ECO:0000303|PubMed:19219229, ECO:0000312|EMBL:ABM05493.1};
OS   Scylla serrata (Mud crab).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Brachyura;
OC   Eubrachyura; Portunoidea; Portunidae; Scylla.
OX   NCBI_TaxID=6761;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ABM05493.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 64-72; 92-98 AND 103-112,
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Hemocyte {ECO:0000269|PubMed:19219229};
RX   PubMed=19219229;
RA   Yedery R.D., Reddy K.V.R.;
RT   "Purification and characterization of antibacterial proteins from granular
RT   hemocytes of Indian mud crab, Scylla serrata.";
RL   Acta Biochim. Pol. 56:71-82(2009).
CC   -!- FUNCTION: Has antibacterial activity against the Gram-positive bacteria
CC       E.coli (MIC<50 ug/ml) and P.aeruginosa (MIC<25 ug/ml), and the Gram-
CC       negative bacteria S.aureus (MIC<100 ug/ml) and S.pyogenes (MIC<50
CC       ug/ml). {ECO:0000269|PubMed:19219229}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19219229}.
CC   -!- TISSUE SPECIFICITY: Strongly expressed in gills, hemocytes and
CC       reproductive tract, with weaker expression in muscle, heart and
CC       digestive tract. Not detected in eyes and hepatopancreas (at protein
CC       level). {ECO:0000269|PubMed:19219229}.
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DR   EMBL; EF137865; ABM05493.1; -; mRNA.
DR   AlphaFoldDB; A1YV58; -.
DR   SMR; A1YV58; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   Gene3D; 3.30.160.320; -; 1.
DR   InterPro; IPR024509; Anti-LPS_factor/Scygonadin.
DR   InterPro; IPR038539; Anti-LPS_factor/Scygonadin_sf.
DR   Pfam; PF11630; DUF3254; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..126
FT                   /note="Antimicrobial protein 1"
FT                   /id="PRO_0000378057"
SQ   SEQUENCE   126 AA;  13901 MW;  6253B1978B6DB440 CRC64;
     MRSSLLLGLT VVLLLGVTVP PCMAGQALNK LMPKIVSAII YMIGQPNAGV TFLGHQCLVE
     STRQPDGFYT AKMWCTSWTS DNPIVGEGRS RVELEALKGS IRNFVQTASD YKKFTIEEVE
     DWIASY