ID   AMP2_PAEAL              Reviewed;          12 AA.
AC   B3EWQ7;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 1.
DT   11-DEC-2019, entry version 6.
DE   RecName: Full=Antimicrobial protein AN5-2 {ECO:0000303|PubMed:24272828};
OS   Paenibacillus alvei (Bacillus alvei).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=44250 {ECO:0000303|PubMed:24272828};
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MASS
RP   SPECTROMETRY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=AN5 {ECO:0000303|PubMed:24272828};
RX   PubMed=24272828; DOI=10.1007/s11274-013-1558-z;
RA   Alkotaini B., Anuar N., Kadhum A.A., Sani A.A.;
RT   "Isolation and identification of a new intracellular antimicrobial peptide
RT   produced by Paenibacillus alvei AN5.";
RL   World J. Microbiol. Biotechnol. 30:1377-1385(2014).
CC   -!- FUNCTION: Has bactericidal activity against Gram-positive bacteria
CC       L.plantarum ATCC 8014 and B.cereus ATCC 14579 as well as against Gram-
CC       negative bacteria E.coli ATCC 25922 and S.enteritidis ATCC 13076.
CC       {ECO:0000269|PubMed:24272828}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Active between pH 2 and 12. {ECO:0000269|PubMed:24272828};
CC       Temperature dependence:
CC         Active between 40 and 90 degrees Celsius. Activity is reduced at 90
CC         degrees Celsius and absent at 100 degrees Celsius.
CC         {ECO:0000269|PubMed:24272828};
CC   -!- MASS SPECTROMETRY: Mass=1330.77; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:24272828};
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DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing.
FT   PEPTIDE         1..12
FT                   /note="Antimicrobial protein AN5-2"
FT                   /evidence="ECO:0000269|PubMed:24272828"
FT                   /id="PRO_0000419698"
SQ   SEQUENCE   12 AA;  1332 MW;  49A5206EE9673767 CRC64;
     FCKSLPLPLS VK