GMM_SALTY
ID GMM_SALTY Reviewed; 157 AA.
AC Q9F7A2; Q8ZNR0;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=GDP-mannose mannosyl hydrolase {ECO:0000255|HAMAP-Rule:MF_00941};
DE Short=GDPMH {ECO:0000255|HAMAP-Rule:MF_00941};
DE EC=3.6.1.- {ECO:0000255|HAMAP-Rule:MF_00941};
GN Name=gmm {ECO:0000255|HAMAP-Rule:MF_00941}; Synonyms=nudD, wcaH;
GN OrderedLocusNames=STM2107;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=11004393; DOI=10.1111/j.1574-6968.2000.tb09312.x;
RA Stevenson G., Lan R., Reeves P.R.;
RT "The colanic acid gene cluster of Salmonella enterica has a complex
RT history.";
RL FEMS Microbiol. Lett. 191:11-16(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Hydrolyzes GDP-mannose. {ECO:0000255|HAMAP-Rule:MF_00941}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GDP-alpha-D-mannose + H2O = D-mannose + GDP + H(+);
CC Xref=Rhea:RHEA:28102, ChEBI:CHEBI:4208, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57527, ChEBI:CHEBI:58189;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00941};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00941};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00941};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00941}.
CC -!- SIMILARITY: Belongs to the Nudix hydrolase family. {ECO:0000255|HAMAP-
CC Rule:MF_00941}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL21011.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF285084; AAG24815.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21011.1; ALT_FRAME; Genomic_DNA.
DR RefSeq; WP_001526160.1; NC_003197.2.
DR AlphaFoldDB; Q9F7A2; -.
DR SMR; Q9F7A2; -.
DR STRING; 90371.CY43_11365; -.
DR PaxDb; Q9F7A2; -.
DR EnsemblBacteria; AAL21011; AAL21011; STM2107.
DR HOGENOM; CLU_037162_12_0_6; -.
DR OMA; HDNSRAY; -.
DR PhylomeDB; Q9F7A2; -.
DR BioCyc; SENT99287:STM2107-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0008727; F:GDP-mannose mannosyl hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR CDD; cd03430; GDPMH; 1.
DR HAMAP; MF_00941; GDPMH_gmm; 1.
DR InterPro; IPR033715; GDPMH.
DR InterPro; IPR028613; GDPMH_Gmm.
DR InterPro; IPR015797; NUDIX_hydrolase-like_dom_sf.
DR InterPro; IPR020084; NUDIX_hydrolase_CS.
DR InterPro; IPR000086; NUDIX_hydrolase_dom.
DR Pfam; PF00293; NUDIX; 1.
DR PIRSF; PIRSF037599; GDPMH; 1.
DR SUPFAM; SSF55811; SSF55811; 1.
DR PROSITE; PS51462; NUDIX; 1.
DR PROSITE; PS00893; NUDIX_BOX; 1.
PE 3: Inferred from homology;
KW Hydrolase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..157
FT /note="GDP-mannose mannosyl hydrolase"
FT /id="PRO_0000056986"
FT DOMAIN 3..153
FT /note="Nudix hydrolase"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00941"
FT MOTIF 50..71
FT /note="Nudix box"
FT BINDING 2..3
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00941"
FT BINDING 8
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00941"
FT BINDING 36
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00941"
FT BINDING 49
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00941"
FT BINDING 69
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00941"
FT BINDING 122
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00941"
SQ SEQUENCE 157 AA; 17650 MW; 75A9CD0472222485 CRC64;
MFLRQEDFAA VVRATPLISL DFIVENGQGE ILLGQRLNRP AQGYWFVPGG RVCKDETLEA
AFARLTQAEL GVRLPLAAGT FYGVWQHFYD DNFSGEDFST HYIVLGFRLR VAESDLRLPD
AQHGSYRWLT PEQLLASDNV HENSRAYFSP DAPAVGL
