GMSA_DICDI
ID GMSA_DICDI Reviewed; 448 AA.
AC Q54ME1;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Gamete and mating-type specific protein A;
DE EC=3.4.22.-;
DE Flags: Precursor;
GN Name=gmsA; ORFNames=DDB_G0286015;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP DISRUPTION PHENOTYPE, FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=12963116; DOI=10.1016/s0925-4773(03)00136-9;
RA Muramoto T., Suzuki K., Shimizu H., Kohara Y., Kohriki E., Obara S.,
RA Tanaka Y., Urushihara H.;
RT "Construction of a gamete-enriched gene pool and RNAi-mediated functional
RT analysis in Dictyostelium discoideum.";
RL Mech. Dev. 120:965-975(2003).
CC -!- FUNCTION: Thiol protease that seems to be involved in the sexual
CC development. {ECO:0000269|PubMed:12963116}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expression is strongly enhanced in the gametes.
CC {ECO:0000269|PubMed:12963116}.
CC -!- DISRUPTION PHENOTYPE: Suppression of cell fusion.
CC {ECO:0000269|PubMed:12963116}.
CC -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU10088}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAFI02000085; EAL64386.1; -; Genomic_DNA.
DR RefSeq; XP_637893.1; XM_632801.1.
DR AlphaFoldDB; Q54ME1; -.
DR SMR; Q54ME1; -.
DR MEROPS; C01.A52; -.
DR PaxDb; Q54ME1; -.
DR EnsemblProtists; EAL64386; EAL64386; DDB_G0286015.
DR GeneID; 8625403; -.
DR KEGG; ddi:DDB_G0286015; -.
DR dictyBase; DDB_G0286015; gmsA.
DR eggNOG; KOG1543; Eukaryota.
DR HOGENOM; CLU_611690_0_0_1; -.
DR InParanoid; Q54ME1; -.
DR OMA; YVDSAFQ; -.
DR PhylomeDB; Q54ME1; -.
DR PRO; PR:Q54ME1; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0009897; C:external side of plasma membrane; ISS:dictyBase.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0008234; F:cysteine-type peptidase activity; ISS:dictyBase.
DR GO; GO:0000747; P:conjugation with cellular fusion; IMP:dictyBase.
DR GO; GO:0051603; P:proteolysis involved in protein catabolic process; IBA:GO_Central.
DR GO; GO:0019953; P:sexual reproduction; IEP:dictyBase.
DR CDD; cd02248; Peptidase_C1A; 1.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR000668; Peptidase_C1A_C.
DR InterPro; IPR039417; Peptidase_C1A_papain-like.
DR Pfam; PF00188; CAP; 1.
DR Pfam; PF00112; Peptidase_C1; 1.
DR PRINTS; PR00705; PAPAIN.
DR SMART; SM00645; Pept_C1; 1.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Protease; Reference proteome; Secreted; Signal;
KW Thiol protease.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..448
FT /note="Gamete and mating-type specific protein A"
FT /id="PRO_0000388254"
FT DOMAIN 30..157
FT /note="SCP"
FT REGION 171..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..235
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 262
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10088"
FT ACT_SITE 397
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10088"
FT ACT_SITE 415
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10088"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 98
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 119
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 448 AA; 48674 MW; E210BB963E724BF1 CRC64;
MKLILVLLCL ISTLFVVKGG LSPTEQQIIV SYHNKWRSSP IGPTPSTTIP ALKWNATIAA
ALQSSLDKCD GKFSTMSQYG TNSEWQSWWT PNTYFNLNAT LDNIQKGASF YDWNAKGCNS
SANYNCQLWT YAVWSKSTSY GCAKTICPDK SEVSCSYYPA GGFKGVLPYT PKTTTPAPTT
PAPTTPKPTT PAPTTPKPTT PAPTTPKPTT PAPTTPKPTT PAPTTPKPTT PAPTTPAPTS
TLTVDWTSYQ TPIRDQGQCG SCWAFASSAA LESRYLIKYG TAQKSTLQLS NQNAVNCIAS
GCNGGWSGNY FNFFKTPGIA YEKDDPYKAV TGTSCITTSS VARFKYTNYG YTEKTKAALL
AELKKGPVTI AVYVDSAFQN YKSGIYNSAT KYTGINHLVL LVGYDQATDA YKIKNSWGSW
WGESGYMRIT ASNDNLAIFA YNSYYPTF
