GMT2_YEAS6
ID GMT2_YEAS6 Reviewed; 249 AA.
AC B5VHH5;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 25-MAY-2022, entry version 46.
DE RecName: Full=Probable GDP-mannose transporter 2;
DE Short=GMT 2;
GN Name=HVG1; Synonyms=YEM9; ORFNames=AWRI1631_51120;
OS Saccharomyces cerevisiae (strain AWRI1631) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=545124;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AWRI1631;
RX PubMed=18778279; DOI=10.1111/j.1567-1364.2008.00434.x;
RA Borneman A.R., Forgan A.H., Pretorius I.S., Chambers P.J.;
RT "Comparative genome analysis of a Saccharomyces cerevisiae wine strain.";
RL FEMS Yeast Res. 8:1185-1195(2008).
CC -!- FUNCTION: Involved in the import of GDP-mannose from the cytoplasm into
CC the Golgi lumen. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Cytoplasmic vesicle membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Endoplasmic
CC reticulum membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Recycles between the Golgi apparatus and the
CC endoplasmic reticulum. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. SLC35D subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: This is a truncated version of GDP-mannose transporter 2. This
CC strain has a stop codon in position 73, which disrupts the gene coding
CC for this protein and produces two ORFs. A contiguous sequence for GDP-
CC mannose transporter 2 can be found in strain Lalvin EC1118 (AC C8Z742).
CC {ECO:0000305}.
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DR EMBL; ABSV01000663; EDZ72621.1; -; Genomic_DNA.
DR AlphaFoldDB; B5VHH5; -.
DR SMR; B5VHH5; -.
DR PRIDE; B5VHH5; -.
DR Proteomes; UP000008988; Unassembled WGS sequence.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005458; F:GDP-mannose transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR038736; Vrg4-like.
DR PANTHER; PTHR11132:SF251; PTHR11132:SF251; 1.
DR Pfam; PF00892; EamA; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle; Endoplasmic reticulum; Glycoprotein; Golgi apparatus;
KW Membrane; Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..249
FT /note="Probable GDP-mannose transporter 2"
FT /id="PRO_0000391671"
FT TOPO_DOM 1..15
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..47
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..84
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..122
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..159
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..211
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..249
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 149
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 249 AA; 27688 MW; 53A0FB1F5049A6BC CRC64;
MIYTSSKSLQ YLAVPIYTIF KNLTIILIAY GEVLFFGGKV TSMELTSFIM MVLSSVVATW
GDQQAIAIKA SSLEDLDQEL VESTIFVLNP GYLWMFTNCI SSALFVLIMR KRIRLTNFKD
YDTMFYNNVL ALPLLLVFSF IMEDWSTKNL SVNLSADSLA AMVISGLMSV GISYCSGWCV
RVTSSTTYSM VGALNKLPIA LAGLVFFDAP KNFLSFFSIF LGFLSGLLYA VAKQKKIQQQ
KVLAATLEK
