GMT2_YEAST
ID GMT2_YEAST Reviewed; 249 AA.
AC P0CE11; D3DLT8; P40027; Q3E844;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-MAR-2010, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Probable GDP-mannose transporter 2;
DE Short=GMT 2;
GN Name=HVG1; Synonyms=YEM9; OrderedLocusNames=YER039C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP GENE NAME.
RX PubMed=9395539; DOI=10.1074/jbc.272.50.31908;
RA Dean N., Zhang Y.B., Poster J.B.;
RT "The VRG4 gene is required for GDP-mannose transport into the lumen of the
RT Golgi in the yeast, Saccharomyces cerevisiae.";
RL J. Biol. Chem. 272:31908-31914(1997).
CC -!- FUNCTION: Involved in the import of GDP-mannose from the cytoplasm into
CC the Golgi lumen. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Cytoplasmic vesicle membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Endoplasmic
CC reticulum membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Recycles between the Golgi apparatus and the
CC endoplasmic reticulum. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. SLC35D subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: This is a truncated version of GDP-mannose transporter 2.
CC Strain S288c has a stop codon in position 73, which disrupts the gene
CC coding for this protein and produces two ORFs YER039C-A and YER039C. A
CC contiguous sequence for GDP-mannose transporter 2 can be found in
CC strain Lalvin EC1118 (AC C8Z742). {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U18796; AAB64574.1; -; Genomic_DNA.
DR EMBL; AY558452; AAS56778.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07692.1; -; Genomic_DNA.
DR PIR; S50542; S50542.
DR RefSeq; NP_010956.1; NM_001178930.1.
DR AlphaFoldDB; P0CE11; -.
DR SMR; P0CE11; -.
DR BioGRID; 36774; 51.
DR STRING; 4932.YER039C; -.
DR PaxDb; P0CE11; -.
DR PRIDE; P0CE11; -.
DR EnsemblFungi; YER039C_mRNA; YER039C; YER039C.
DR GeneID; 856761; -.
DR KEGG; sce:YER039C; -.
DR SGD; S000000841; HVG1.
DR VEuPathDB; FungiDB:YER039C; -.
DR eggNOG; KOG1444; Eukaryota.
DR GeneTree; ENSGT00510000048348; -.
DR HOGENOM; CLU_025360_1_1_1; -.
DR InParanoid; P0CE11; -.
DR OMA; HEWEYLY; -.
DR BioCyc; YEAST:G3O-30220-MON; -.
DR PRO; PR:P0CE11; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P0CE11; protein.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0005458; F:GDP-mannose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR InterPro; IPR000620; EamA_dom.
DR InterPro; IPR038736; Vrg4-like.
DR PANTHER; PTHR11132:SF251; PTHR11132:SF251; 1.
DR Pfam; PF00892; EamA; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle; Endoplasmic reticulum; Glycoprotein; Golgi apparatus;
KW Membrane; Reference proteome; Sugar transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..249
FT /note="Probable GDP-mannose transporter 2"
FT /id="PRO_0000202628"
FT TOPO_DOM 1..15
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..47
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..84
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..122
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..159
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..211
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..249
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 149
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 249 AA; 27688 MW; 53A0FB1F5049A6BC CRC64;
MIYTSSKSLQ YLAVPIYTIF KNLTIILIAY GEVLFFGGKV TSMELTSFIM MVLSSVVATW
GDQQAIAIKA SSLEDLDQEL VESTIFVLNP GYLWMFTNCI SSALFVLIMR KRIRLTNFKD
YDTMFYNNVL ALPLLLVFSF IMEDWSTKNL SVNLSADSLA AMVISGLMSV GISYCSGWCV
RVTSSTTYSM VGALNKLPIA LAGLVFFDAP KNFLSFFSIF LGFLSGLLYA VAKQKKIQQQ
KVLAATLEK
