GMT_CANAL
ID GMT_CANAL Reviewed; 371 AA.
AC Q5A477; A0A1D8PE59; Q96WN8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=GDP-mannose transporter;
DE Short=GMT;
GN Name=VRG4; OrderedLocusNames=CAALFM_C107700CA;
GN ORFNames=CaO19.1232, CaO19.8817;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=SC5314 / CAI4 / ATCC MYA-682;
RX PubMed=11741841; DOI=10.1128/jb.184.1.29-42.2002;
RA Nishikawa A., Poster J.B., Jigami Y., Dean N.;
RT "Molecular and phenotypic analysis of CaVRG4, encoding an essential Golgi
RT apparatus GDP-mannose transporter.";
RL J. Bacteriol. 184:29-42(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: Involved in the import of GDP-mannose from the cytoplasm into
CC the Golgi lumen. Involved in hyphal formation.
CC {ECO:0000269|PubMed:11741841}.
CC -!- SUBUNIT: Homooligomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:11741841}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11741841}. Cytoplasmic vesicle membrane
CC {ECO:0000269|PubMed:11741841}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11741841}. Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:11741841}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11741841}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. SLC35D subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF164627; AAK74075.1; -; Genomic_DNA.
DR EMBL; CP017623; AOW26417.1; -; Genomic_DNA.
DR RefSeq; XP_716540.2; XM_711447.2.
DR AlphaFoldDB; Q5A477; -.
DR SMR; Q5A477; -.
DR STRING; 237561.Q5A477; -.
DR TCDB; 2.A.7.13.2; the drug/metabolite transporter (dmt) superfamily.
DR GeneID; 3641768; -.
DR KEGG; cal:CAALFM_C107700CA; -.
DR CGD; CAL0000182900; VRG4.
DR VEuPathDB; FungiDB:C1_07700C_A; -.
DR eggNOG; KOG1444; Eukaryota.
DR HOGENOM; CLU_025360_1_2_1; -.
DR InParanoid; Q5A477; -.
DR OrthoDB; 1093260at2759; -.
DR PRO; PR:Q5A477; -.
DR Proteomes; UP000000559; Chromosome 1.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IDA:CGD.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0005458; F:GDP-mannose transmembrane transporter activity; IGI:CGD.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:1990570; P:GDP-mannose transmembrane transport; IGI:CGD.
DR GO; GO:0030448; P:hyphal growth; IMP:CGD.
DR GO; GO:0006486; P:protein glycosylation; IMP:CGD.
DR InterPro; IPR038736; Vrg4-like.
DR PANTHER; PTHR11132:SF251; PTHR11132:SF251; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle; Endoplasmic reticulum; Glycoprotein; Golgi apparatus;
KW Membrane; Reference proteome; Sugar transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..371
FT /note="GDP-mannose transporter"
FT /id="PRO_0000333515"
FT TOPO_DOM 1..51
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..80
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..121
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 122..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..145
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 146..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..171
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..206
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..241
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..281
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..309
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 310..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..332
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 333..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT CARBOHYD 268
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 30
FT /note="H -> Y (in Ref. 1; AAK74075)"
SQ SEQUENCE 371 AA; 41236 MW; 4BD03B8DF7AB881C CRC64;
MGVISFYLIG QLLYLIRKKY TTTYRQQQQH QYNMDSKHST SSSSSGSLAT RISNSGPISI
AAYCLSSILM TVTNKYVLSG FSFNLNFFLL AVQSIVCIVT IGSLKSLNII TYRQFNKDEA
KKWSPIAFLL VAMIYTSSKA LQYLSIPVYT IFKNLTIILI AYGEVIWFGG KVTTMALSSF
LLMVLSSVIA YYGDNAAVKS HDDAFALYLG YFWMLTNCFA SAAFVLIMRK RIKLTNFKDF
DTMYYNNLLS IPILLICSFI FEDWSSANVS LNFPADNRVT TITAMILSGA SSVGISYCSA
WCVRVTSSTT YSMVGALNKL PIALSGLIFF EAAVNFWSVS SIFVGFGAGL VYAVAKQKQQ
KEQSQQLPTT K
