ID   GMT_PICPA               Reviewed;         329 AA.
AC   Q2AAF6;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=GDP-mannose transporter;
DE            Short=GMT;
GN   Name=VIG4; Synonyms=VRG4;
OS   Komagataella pastoris (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=4922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RX   PubMed=16960330; DOI=10.2323/jgam.52.137;
RA   Arakawa K., Abe M., Noda Y., Adachi H., Yoda K.;
RT   "Molecular cloning and characterization of a Pichia pastoris ortholog of
RT   the yeast Golgi GDP-mannose transporter gene.";
RL   J. Gen. Appl. Microbiol. 52:137-145(2006).
CC   -!- FUNCTION: Involved in the import of GDP-mannose from the cytoplasm into
CC       the Golgi lumen. {ECO:0000250}.
CC   -!- SUBUNIT: Homooligomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:16960330}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:16960330}. Cytoplasmic vesicle membrane
CC       {ECO:0000269|PubMed:16960330}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:16960330}. Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:16960330}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:16960330}.
CC   -!- SIMILARITY: Belongs to the TPT transporter family. SLC35D subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB252397; BAE80635.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q2AAF6; -.
DR   SMR; Q2AAF6; -.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005458; F:GDP-mannose transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR   InterPro; IPR038736; Vrg4-like.
DR   PANTHER; PTHR11132:SF251; PTHR11132:SF251; 1.
PE   3: Inferred from homology;
KW   Cytoplasmic vesicle; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW   Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..329
FT                   /note="GDP-mannose transporter"
FT                   /id="PRO_0000333534"
FT   TOPO_DOM        1..11
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        33..40
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..83
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        105..107
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        129..131
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        153..163
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        185..196
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        218..237
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        259..266
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        267..287
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        288..290
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        291..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        312..329
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   329 AA;  36354 MW;  088D373900F389C0 CRC64;
     MADKGSVAAK SLTNSAPLSI FSYCAASILM TVTNKYAVSG VDFNFNFFLL AVQGIVCITL
     ISSLKQLNVI TFREFNKVEA KKWFPIAVLL VVMIYTSSKA LQYLSIPIYT IFKNLTIILI
     AYGEVIWFGG RVTNLALGSF VLMVLSSAVA SYGDSNVDTG KLNFNIGYFW MFTNCFSSAA
     FVLFMRKRIK LTNFKDFDTM YYNNLLSIPI LLFASLTTED WSAKNIAQNF PEDTKYAVIA
     SMIISGMSAV GISYTSAWCV RVTSSTTYSM VGALNKLPIA LSGLLFFKAP INFYSISSIF
     IGFAAGLVYA IAKQKQKKED ELQLPTDKS