GM_HCMVA
ID GM_HCMVA Reviewed; 372 AA.
AC P16733; Q7M6T7;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 23-FEB-2022, entry version 89.
DE RecName: Full=Envelope glycoprotein M {ECO:0000255|HAMAP-Rule:MF_04035};
DE Short=gM {ECO:0000255|HAMAP-Rule:MF_04035};
GN Name=gM {ECO:0000255|HAMAP-Rule:MF_04035}; ORFNames=UL100;
OS Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX NCBI_TaxID=10360;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2547996; DOI=10.1128/jvi.63.9.3792-3800.1989;
RA Lehner R., Meyer H., Mach M.;
RT "Identification and characterization of a human cytomegalovirus gene coding
RT for a membrane protein that is conserved among human herpesviruses.";
RL J. Virol. 63:3792-3800(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=2161319; DOI=10.1007/978-3-642-74980-3_6;
RA Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R.,
RA Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A.,
RA Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.;
RT "Analysis of the protein-coding content of the sequence of human
RT cytomegalovirus strain AD169.";
RL Curr. Top. Microbiol. Immunol. 154:125-169(1990).
RN [3]
RP GENOME REANNOTATION.
RX PubMed=12533697; DOI=10.1099/vir.0.18606-0;
RA Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA McGeoch D.J., Hayward G.S.;
RT "The human cytomegalovirus genome revisited: comparison with the chimpanzee
RT cytomegalovirus genome.";
RL J. Gen. Virol. 84:17-28(2003).
RN [4]
RP ERRATUM OF PUBMED:12533697.
RA Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA McGeoch D.J., Hayward G.S.;
RL J. Gen. Virol. 84:1053-1053(2003).
RN [5]
RP IDENTIFICATION.
RX PubMed=15452216; DOI=10.1128/jvi.78.20.10960-10966.2004;
RA Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J.,
RA Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W.,
RA Shenk T., Smith R.D., Nelson J.A.;
RT "Identification of proteins in human cytomegalovirus (HCMV) particles: the
RT HCMV proteome.";
RL J. Virol. 78:10960-10966(2004).
RN [6]
RP ERRATUM OF PUBMED:15452216.
RA Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J.,
RA Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W.,
RA Shenk T., Smith R.D., Nelson J.A.;
RL J. Virol. 78:13395-13395(2004).
RN [7]
RP SUBUNIT, SUBCELLULAR LOCATION, AND DISULFIDE BOND.
RX PubMed=11090188; DOI=10.1128/jvi.74.24.11881-11892.2000;
RA Mach M., Kropff B., Dal Monte P., Britt W.;
RT "Complex formation by human cytomegalovirus glycoproteins M (gpUL100) and N
RT (gpUL73).";
RL J. Virol. 74:11881-11892(2000).
RN [8]
RP SUBUNIT, AND DISULFIDE BOND.
RX PubMed=15681419; DOI=10.1128/jvi.79.4.2160-2170.2005;
RA Mach M., Kropff B., Kryzaniak M., Britt W.;
RT "Complex formation by glycoproteins M and N of human cytomegalovirus:
RT structural and functional aspects.";
RL J. Virol. 79:2160-2170(2005).
RN [9]
RP INTERACTION WITH HOST RAB11FIP4.
RX PubMed=19761540; DOI=10.1111/j.1600-0854.2009.00967.x;
RA Krzyzaniak M.A., Mach M., Britt W.J.;
RT "HCMV-encoded glycoprotein M (UL100) interacts with Rab11 effector protein
RT FIP4.";
RL Traffic 10:1439-1457(2009).
CC -!- FUNCTION: Envelope glycoprotein important for virion assembly and
CC egress. Plays a role in the correct incorporation of gH-gL into virion
CC membrane. Directs the glycoprotein N (gN) to the host trans-Golgi
CC network. {ECO:0000255|HAMAP-Rule:MF_04035, ECO:0000269|PubMed:11090188,
CC ECO:0000269|PubMed:15681419, ECO:0000269|PubMed:19761540}.
CC -!- SUBUNIT: Interacts (via N-terminus) with gN (via N-terminus). The gM-gN
CC heterodimer forms the gCII complex. Interacts with host RAB11FIP4.
CC {ECO:0000255|HAMAP-Rule:MF_04035, ECO:0000269|PubMed:11090188,
CC ECO:0000269|PubMed:15681419, ECO:0000269|PubMed:19761540}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000255|HAMAP-Rule:MF_04035,
CC ECO:0000305|PubMed:11090188}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_04035, ECO:0000305|PubMed:11090188}. Host
CC Golgi apparatus, host trans-Golgi network {ECO:0000255|HAMAP-
CC Rule:MF_04035, ECO:0000305|PubMed:11090188}. Host endosome membrane
CC {ECO:0000255|HAMAP-Rule:MF_04035}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_04035, ECO:0000305|PubMed:11090188}. Host
CC nucleus inner membrane {ECO:0000255|HAMAP-Rule:MF_04035}; Multi-pass
CC membrane protein {ECO:0000255|HAMAP-Rule:MF_04035,
CC ECO:0000305|PubMed:11090188}. Note=During virion morphogenesis, this
CC protein accumulates in the trans-Golgi network where secondary
CC envelopment occurs. {ECO:0000255|HAMAP-Rule:MF_04035}.
CC -!- SIMILARITY: Belongs to the herpesviridae glycoprotein M family.
CC {ECO:0000255|HAMAP-Rule:MF_04035}.
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DR EMBL; M28350; AAA45984.1; -; Genomic_DNA.
DR EMBL; X17403; CAA35336.1; -; Genomic_DNA.
DR EMBL; BK000394; DAA00097.1; -; Genomic_DNA.
DR PIR; S09865; QQBEJ7.
DR SMR; P16733; -.
DR Proteomes; UP000008991; Genome.
DR Proteomes; UP000008992; Genome.
DR GO; GO:0044175; C:host cell endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0044201; C:host cell nuclear inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-UniRule.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR HAMAP; MF_04035; HSV_GM; 1.
DR InterPro; IPR000785; Herpes_glycop_M.
DR Pfam; PF01528; Herpes_glycop; 1.
DR PRINTS; PR00333; HSVINTEGRLMP.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Host endosome; Host Golgi apparatus;
KW Host membrane; Host nucleus; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Viral envelope protein; Virion.
FT CHAIN 1..372
FT /note="Envelope glycoprotein M"
FT /id="PRO_0000115785"
FT TOPO_DOM 1..13
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TOPO_DOM 35..79
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TOPO_DOM 101..126
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TOPO_DOM 148..151
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TOPO_DOM 173..200
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TOPO_DOM 222..239
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TOPO_DOM 261..264
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TOPO_DOM 286..298
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT TOPO_DOM 320..372
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04035"
FT REGION 348..372
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 44
FT /note="Interchain (with C-90 in gN)"
FT /evidence="ECO:0000269|PubMed:11090188,
FT ECO:0000269|PubMed:15681419"
SQ SEQUENCE 372 AA; 42861 MW; F2F99BEC69BF8E32 CRC64;
MAPSHVDKVN TRTWSASIVF MVLTFVNVSV HLVLSNFPHL GYPCVYYHVV DFERLNMSAY
NVMHLHTPML FLDSVQLVCY AVFMQLVFLA VTIYYLVCWI KISMRKDKGM SLNQSTRDIS
YMGDSLTAFL FILSMDTFQL FTLTMSFRLP SMIAFMAAVH FFCLTIFNVS MVTQYRSYKR
SLFFFSRLHP KLKGTVQFRT LIVNLVEVAL GFNTTVVAMA LCYGFGNNFF VRTGHMVLAV
FVVYAIISII YFLLIEAVFF QYVKVQFGYH LGAFFGLCGL IYPIVQYDTF LSNEYRTGIS
WSFGMLFFIW AMFTTCRAVR YFRGRGSGSV KYQALATASG EEVAVLSHHD SLESRRLREE
EDDDDDEDFE DA
