位置:首页 > 蛋白库 > GNAI3_CRIGR
GNAI3_CRIGR
ID   GNAI3_CRIGR             Reviewed;          63 AA.
AC   Q60397;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Guanine nucleotide-binding protein G(i) subunit alpha-3;
DE   AltName: Full=G(i) alpha-3;
DE   Flags: Fragment;
GN   Name=GNAI3;
OS   Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC   Cricetidae; Cricetinae; Cricetulus.
OX   NCBI_TaxID=10029;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7698751; DOI=10.1006/geno.1994.1618;
RA   Baron B., Fernandez M.A., Toledo F., le Roscouet D., Mayau V., Martin N.,
RA   Buttin G., Debatisse M.;
RT   "The highly conserved Chinese hamster GNAI3 gene maps less than 60 kb from
RT   the AMPD2 gene and lacks the intronic U6 snRNA present in its human
RT   counterpart.";
RL   Genomics 24:288-294(1994).
CC   -!- FUNCTION: Heterotrimeric guanine nucleotide-binding proteins (G
CC       proteins) function as transducers downstream of G protein-coupled
CC       receptors (GPCRs) in numerous signaling cascades. The alpha chain
CC       contains the guanine nucleotide binding site and alternates between an
CC       active, GTP-bound state and an inactive, GDP-bound state. Signaling by
CC       an activated GPCR promotes GDP release and GTP binding. The alpha
CC       subunit has a low GTPase activity that converts bound GTP to GDP,
CC       thereby terminating the signal. Both GDP release and GTP hydrolysis are
CC       modulated by numerous regulatory proteins. Signaling is mediated via
CC       effector proteins, such as adenylate cyclase. Inhibits adenylate
CC       cyclase activity, leading to decreased intracellular cAMP levels.
CC       Stimulates the activity of receptor-regulated K(+) channels. The active
CC       GTP-bound form prevents the association of RGS14 with centrosomes and
CC       is required for the translocation of RGS14 from the cytoplasm to the
CC       plasma membrane. May play a role in cell division.
CC       {ECO:0000250|UniProtKB:P08754}.
CC   -!- SUBUNIT: Heterotrimeric G proteins are composed of 3 units; alpha, beta
CC       and gamma. The alpha subunit contains the guanine nucleotide binding
CC       site. GTP binding causes dissociation of the heterotrimer, liberating
CC       the individual subunits so that they can interact with downstream
CC       effector proteins. Forms a complex with CCDC88A/GIV and EGFR which
CC       leads to enhanced EGFR signaling and triggering of cell migration;
CC       ligand stimulation is required for recruitment of GNAI3 to the complex
CC       (By similarity). Interacts (inactive GDP-bound form) with CCDC88A/GIV
CC       (via GBA motif); the interaction leads to activation of GNAI3 (By
CC       similarity). Interacts (inactive GDP-bound form) with CCDC88C/DAPLE
CC       (via GBA motif); the interaction leads to activation of GNAI3 (By
CC       similarity). Interacts (inactive GDP-bound form) with NUCB1 (via GBA
CC       motif) and NUCB2 (via GBA motif); the interaction leads to activation
CC       of GNAI3 (By similarity). Interacts (inactive GDP-bound form) with
CC       PLCD4 (via GBA motif); the interaction leads to activation of GNAI3 (By
CC       similarity). Interacts with INSR; the interaction is probably mediated
CC       by CCDC88A/GIV (By similarity). Interacts with GPSM1. Interacts (GDP-
CC       bound form) with GPSM2 (via GoLoco domains). Does not interact with
CC       RGS2. Interacts with RGS8 and RGS10; this strongly enhances the
CC       intrinsic GTPase activity. Interacts with RGS12. Interacts with RGS16;
CC       this strongly enhances the intrinsic GTPase activity. Interacts (via
CC       active GTP- or inactive GDP-bound form) with RGS14.
CC       {ECO:0000250|UniProtKB:P08753, ECO:0000250|UniProtKB:P08754,
CC       ECO:0000250|UniProtKB:Q9DC51}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P08754}. Cell
CC       membrane {ECO:0000250|UniProtKB:P08754}; Lipid-anchor {ECO:0000305}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC       {ECO:0000250|UniProtKB:P08754}. Note=Localizes in the centrosomes of
CC       interphase and mitotic cells. Detected at the cleavage furrow and/or
CC       the midbody. {ECO:0000250|UniProtKB:P08754}.
CC   -!- SIMILARITY: Belongs to the G-alpha family. G(i/o/t/z) subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X79282; CAA55869.1; -; Genomic_DNA.
DR   PIR; I48071; I48071.
DR   AlphaFoldDB; Q60397; -.
DR   SMR; Q60397; -.
DR   STRING; 10029.NP_001230987.1; -.
DR   eggNOG; KOG0082; Eukaryota.
DR   GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IEA:InterPro.
DR   GO; GO:0019003; F:GDP binding; ISS:UniProtKB.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; ISS:UniProtKB.
DR   GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; ISS:UniProtKB.
DR   GO; GO:0007212; P:dopamine receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0046039; P:GTP metabolic process; ISS:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR001408; Gprotein_alpha_I.
DR   InterPro; IPR001019; Gprotein_alpha_su.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10218; PTHR10218; 1.
DR   PRINTS; PR00441; GPROTEINAI.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51882; G_ALPHA; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Cell membrane; Cytoplasm; Cytoskeleton;
KW   GTP-binding; Lipoprotein; Membrane; Nucleotide-binding; Transducer.
FT   CHAIN           <1..63
FT                   /note="Guanine nucleotide-binding protein G(i) subunit
FT                   alpha-3"
FT                   /id="PRO_0000203691"
FT   DOMAIN          <1..63
FT                   /note="G-alpha"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          <1..?1
FT                   /note="G1 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          <1..?1
FT                   /note="G2 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          <1..?1
FT                   /note="G3 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          <1..?1
FT                   /note="G4 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          33..38
FT                   /note="G5 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   BINDING         35
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P08754"
FT   NON_TER         1
SQ   SEQUENCE   63 AA;  7288 MW;  32C3A203F194ADB9 CRC64;
     GSNTYEEAAA YIQCQFEDLN RRKDTKEIYT HFTCATDTKN VQFVFDAVTD VIIKNNLKEC
     GLY
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024