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GNAL_DROME
ID   GNAL_DROME              Reviewed;         457 AA.
AC   P25157; Q7PL79; Q9U3X3;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   03-AUG-2022, entry version 174.
DE   RecName: Full=Guanine nucleotide-binding protein subunit alpha homolog;
DE   AltName: Full=Protein concertina;
GN   Name=cta; Synonyms=CTR; ORFNames=CG17678;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=Oregon-R; TISSUE=Ovary;
RX   PubMed=1899050; DOI=10.1016/0092-8674(91)90652-f;
RA   Parks S., Wieschaus E.;
RT   "The Drosophila gastrulation gene concertina encodes a G alpha-like
RT   protein.";
RL   Cell 64:447-458(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 233-324.
RX   PubMed=10748524; DOI=10.1038/74487;
RA   van Steensel B., Henikoff S.;
RT   "Identification of in vivo DNA targets of chromatin proteins using tethered
RT   dam methyltransferase.";
RL   Nat. Biotechnol. 18:424-428(2000).
CC   -!- FUNCTION: May play a role in a signal transduction pathway used during
CC       gastrulation. Required specifically for the ventral furrow and
CC       posterior midgut invaginations, where it is necessary for coordinating
CC       cell shape changes. {ECO:0000269|PubMed:1899050}.
CC   -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC       as modulators or transducers in various transmembrane signaling
CC       systems. {ECO:0000269|PubMed:1899050}.
CC   -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The
CC       alpha chain contains the guanine nucleotide binding site.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:1899050}.
CC   -!- TISSUE SPECIFICITY: In ovary, expressed in nurse cells and oocyte. In
CC       early embryos, distributed uniformly. At the extended germband stage,
CC       accumulates in the mesoderm. {ECO:0000269|PubMed:1899050}.
CC   -!- DEVELOPMENTAL STAGE: Expressed throughout oogenesis. In early embryos,
CC       expression drops during cellularization, remains low throughout
CC       gastrulation and increases once germband extension has begun.
CC       {ECO:0000269|PubMed:1899050}.
CC   -!- SIMILARITY: Belongs to the G-alpha family. G(12) subfamily.
CC       {ECO:0000305}.
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DR   EMBL; M94285; AAA82939.1; -; mRNA.
DR   EMBL; AE014134; EAA46036.2; -; Genomic_DNA.
DR   EMBL; AY089534; AAL90272.1; -; mRNA.
DR   EMBL; AF211849; AAF17622.1; -; Genomic_DNA.
DR   PIR; A38567; A38567.
DR   RefSeq; NP_001036421.1; NM_001042956.2.
DR   RefSeq; NP_001260696.1; NM_001273767.1.
DR   AlphaFoldDB; P25157; -.
DR   SMR; P25157; -.
DR   BioGRID; 78256; 7.
DR   STRING; 7227.FBpp0110418; -.
DR   PaxDb; P25157; -.
DR   PRIDE; P25157; -.
DR   DNASU; 3355131; -.
DR   EnsemblMetazoa; FBtr0111126; FBpp0110418; FBgn0000384.
DR   EnsemblMetazoa; FBtr0334157; FBpp0306274; FBgn0000384.
DR   GeneID; 3355131; -.
DR   KEGG; dme:Dmel_CG17678; -.
DR   UCSC; CG17678-RA; d. melanogaster.
DR   CTD; 3355131; -.
DR   FlyBase; FBgn0000384; cta.
DR   VEuPathDB; VectorBase:FBgn0000384; -.
DR   eggNOG; KOG0082; Eukaryota.
DR   GeneTree; ENSGT00940000168398; -.
DR   HOGENOM; CLU_014184_3_2_1; -.
DR   InParanoid; P25157; -.
DR   OMA; TSRFACM; -.
DR   OrthoDB; 754573at2759; -.
DR   PhylomeDB; P25157; -.
DR   Reactome; R-DME-193648; NRAGE signals death through JNK.
DR   Reactome; R-DME-416482; G alpha (12/13) signalling events.
DR   Reactome; R-DME-428930; Thromboxane signalling through TP receptor.
DR   Reactome; R-DME-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DME-9013149; RAC1 GTPase cycle.
DR   BioGRID-ORCS; 3355131; 0 hits in 3 CRISPR screens.
DR   ChiTaRS; cta; fly.
DR   GenomeRNAi; 3355131; -.
DR   PRO; PR:P25157; -.
DR   Proteomes; UP000000803; Chromosome 2L.
DR   Bgee; FBgn0000384; Expressed in ovary and 12 other tissues.
DR   ExpressionAtlas; P25157; baseline and differential.
DR   Genevisible; P25157; DM.
DR   GO; GO:0031526; C:brush border membrane; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005834; C:heterotrimeric G-protein complex; ISS:FlyBase.
DR   GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR   GO; GO:0031752; F:D5 dopamine receptor binding; IBA:GO_Central.
DR   GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR   GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; ISS:FlyBase.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0070252; P:actin-mediated cell contraction; IGI:FlyBase.
DR   GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR   GO; GO:0003384; P:apical constriction involved in gastrulation; IGI:FlyBase.
DR   GO; GO:0060027; P:convergent extension involved in gastrulation; IMP:FlyBase.
DR   GO; GO:0003380; P:establishment or maintenance of cytoskeleton polarity involved in gastrulation; IMP:FlyBase.
DR   GO; GO:0010004; P:gastrulation involving germ band extension; IMP:FlyBase.
DR   GO; GO:0048383; P:mesectoderm development; IMP:FlyBase.
DR   GO; GO:0016476; P:regulation of embryonic cell shape; IMP:FlyBase.
DR   GO; GO:0010470; P:regulation of gastrulation; IMP:FlyBase.
DR   GO; GO:0043519; P:regulation of myosin II filament organization; IMP:FlyBase.
DR   GO; GO:0007266; P:Rho protein signal transduction; IBA:GO_Central.
DR   CDD; cd00066; G-alpha; 1.
DR   Gene3D; 1.10.400.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR000469; Gprotein_alpha_12/13.
DR   InterPro; IPR001019; Gprotein_alpha_su.
DR   InterPro; IPR011025; GproteinA_insert.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR10218; PTHR10218; 1.
DR   Pfam; PF00503; G-alpha; 1.
DR   PRINTS; PR00318; GPROTEINA.
DR   PRINTS; PR00440; GPROTEINA12.
DR   SMART; SM00275; G_alpha; 1.
DR   SUPFAM; SSF47895; SSF47895; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51882; G_ALPHA; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Gastrulation; GTP-binding; Magnesium;
KW   Metal-binding; Nucleotide-binding; Reference proteome; Transducer.
FT   CHAIN           1..457
FT                   /note="Guanine nucleotide-binding protein subunit alpha
FT                   homolog"
FT                   /id="PRO_0000203776"
FT   DOMAIN          131..457
FT                   /note="G-alpha"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          134..147
FT                   /note="G1 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          272..280
FT                   /note="G2 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          295..304
FT                   /note="G3 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          365..372
FT                   /note="G4 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   REGION          427..432
FT                   /note="G5 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT   BINDING         139..146
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         146
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         274..280
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         280
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         299..303
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         369..372
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         429
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   457 AA;  52753 MW;  D204415C4BC2CEC7 CRC64;
     MSGITLTKLT QERISIPNNN VITNGVENNI DSDTLSGTLT HLMEEHRTRV GAVTGPEAAT
     TSTDGLISNG AERLRLQGSR LQTSRFACFR CCGNIITYLV RLRSTPEELE QRYKSKEIDK
     FLEKEKHTFR RQVKLLLLGA GESGKSTFLK QMRIIHGVNF DYELLLEYQS VIYQNVIRGM
     QVLLDAREKL NIAWGSDGRE QDAYDAKLME CNSLDVPKFM EYAPPISRLW QDRGIRRAFE
     RRREFQISDS VSYFLDEIQR LATPDYVPTH KDILHCRKAT KGVYEFCVKV QNIPFVFVDV
     GGQRTQRQKW TRCFDSSVTS IIFLVSSSEF DQVLAEDRKT NRLEESKNIF DTIVNNATFK
     GISIILFLNK TDLLEQKVCN PETDIRWYYP HFNGNPHSVL DVQNFILQMF MSVRRSSSIS
     RIYHHFTTAI DTRNINVVFN SVKDTILQRN LNALMLQ
 
 
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