GNKL_PINPS
ID GNKL_PINPS Reviewed; 129 AA.
AC D9IWE0;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 1.
DT 03-AUG-2022, entry version 17.
DE RecName: Full=Antifungal protein ginkbilobin-like protein {ECO:0000305};
DE AltName: Full=Embryo-abundant protein {ECO:0000312|EMBL:ADJ53039.1};
DE Flags: Precursor;
GN Name=EAP {ECO:0000312|EMBL:ADJ53039.1};
OS Pinus pinaster (Maritime pine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC Pinus subgen. Pinus.
OX NCBI_TaxID=71647;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Canales J., Canovas F.M.;
RT "Cloning of ammonium stress related genes.";
RL Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP IDENTIFICATION.
RX PubMed=26315821; DOI=10.1007/s00709-015-0876-4;
RA Gao N., Wadhwani P., Muehlhaeuser P., Liu Q., Riemann M., Ulrich A.S.,
RA Nick P.;
RT "An antifungal protein from Ginkgo biloba binds actin and can trigger cell
RT death.";
RL Protoplasma 253:1159-1174(2016).
CC -!- FUNCTION: Exerts antifungal activity through its carbohydrate-binding
CC specificity. {ECO:0000250|UniProtKB:A4ZDL6}.
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DR EMBL; HM210087; ADJ53039.1; -; mRNA.
DR AlphaFoldDB; D9IWE0; -.
DR SMR; D9IWE0; -.
DR GO; GO:0005537; F:mannose binding; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.430.20; -; 1.
DR InterPro; IPR002902; GNK2.
DR InterPro; IPR038408; GNK2_sf.
DR Pfam; PF01657; Stress-antifung; 1.
DR PROSITE; PS51473; GNK2; 1.
PE 2: Evidence at transcript level;
KW Antibiotic; Antimicrobial; Disulfide bond; Fungicide; Lectin;
KW Mannose-binding; Plant defense; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..129
FT /note="Antifungal protein ginkbilobin-like protein"
FT /id="PRO_5003125649"
FT DOMAIN 27..129
FT /note="Gnk2-homologous"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT BINDING 35
FT /ligand="alpha-D-mannopyranose"
FT /ligand_id="ChEBI:CHEBI:28729"
FT /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT BINDING 114
FT /ligand="alpha-D-mannopyranose"
FT /ligand_id="ChEBI:CHEBI:28729"
FT /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT BINDING 125
FT /ligand="alpha-D-mannopyranose"
FT /ligand_id="ChEBI:CHEBI:28729"
FT /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT DISULFID 34..107
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 83..92
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 95..120
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
SQ SEQUENCE 129 AA; 13683 MW; 7377D0E0EFB32A23 CRC64;
MRYSFGFTLA VMVVALVVGS AVGTPRTNLV SSACNGQKIQ SGSSFYSTLG SLLVDLEGNT
ASSGYDYKAS RAGSPTAYGR AVCNQGLSQS DCTACLKNLG GRIWNICGYA IGARVQLTDC
FIRYEQYSF
