GNL2_ARATH
ID GNL2_ARATH Reviewed; 1375 AA.
AC F4K2K3;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=ARF guanine-nucleotide exchange factor GNL2;
DE AltName: Full=Protein GNOM-like 2;
GN Name=GNL2; Synonyms=GBF2; OrderedLocusNames=At5g19610; ORFNames=T29J13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12553910; DOI=10.1016/s0092-8674(03)00003-5;
RA Geldner N., Anders N., Wolters H., Keicher J., Kornberger W., Muller P.,
RA Delbarre A., Ueda T., Nakano A., Juergens G.;
RT "The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin
RT transport, and auxin-dependent plant growth.";
RL Cell 112:219-230(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14742722; DOI=10.1091/mbc.e03-06-0443;
RA Cox R., Mason-Gamer R.J., Jackson C.L., Segev N.;
RT "Phylogenetic analysis of Sec7-domain-containing Arf nucleotide
RT exchangers.";
RL Mol. Biol. Cell 15:1487-1505(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=17653190; DOI=10.1038/nature05967;
RA Richter S., Geldner N., Schrader J., Wolters H., Stierhof Y.D., Rios G.,
RA Koncz C., Robinson D.G., Juergens G.;
RT "Functional diversification of closely related ARF-GEFs in protein
RT secretion and recycling.";
RL Nature 448:488-492(2007).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=19686373; DOI=10.1111/j.1744-7909.2009.00858.x;
RA Jia D.J., Cao X., Wang W., Tan X.Y., Zhang X.Q., Chen L.Q., Ye D.;
RT "GNOM-LIKE 2, encoding an adenosine diphosphate-ribosylation factor-guanine
RT nucleotide exchange factor protein homologous to GNOM and GNL1, is
RT essential for pollen germination in Arabidopsis.";
RL J. Integr. Plant Biol. 51:762-773(2009).
CC -!- FUNCTION: Activates the ARF proteins by exchanging bound GDP for free
CC GTP. Plays a role in vesicular protein sorting. Essential for pollen
CC germination. {ECO:0000269|PubMed:19686373}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol. Membrane; Peripheral membrane
CC protein; Cytoplasmic side. Note=Soluble and partially membrane-bound.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in mature pollen grains
CC and growing pollen tubes. {ECO:0000269|PubMed:19686373}.
CC -!- DISRUPTION PHENOTYPE: Failure of pollen germination.
CC {ECO:0000269|PubMed:19686373}.
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DR EMBL; AF296838; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92730.1; -; Genomic_DNA.
DR RefSeq; NP_197462.1; NM_121966.2.
DR AlphaFoldDB; F4K2K3; -.
DR SMR; F4K2K3; -.
DR STRING; 3702.AT5G19610.1; -.
DR PaxDb; F4K2K3; -.
DR PRIDE; F4K2K3; -.
DR ProteomicsDB; 248537; -.
DR EnsemblPlants; AT5G19610.1; AT5G19610.1; AT5G19610.
DR GeneID; 832081; -.
DR Gramene; AT5G19610.1; AT5G19610.1; AT5G19610.
DR KEGG; ath:AT5G19610; -.
DR Araport; AT5G19610; -.
DR TAIR; locus:2183159; AT5G19610.
DR eggNOG; KOG0928; Eukaryota.
DR HOGENOM; CLU_001204_1_0_1; -.
DR InParanoid; F4K2K3; -.
DR OMA; RLDTCIK; -.
DR OrthoDB; 815698at2759; -.
DR PhylomeDB; F4K2K3; -.
DR PRO; PR:F4K2K3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4K2K3; baseline and differential.
DR Genevisible; F4K2K3; AT.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0009846; P:pollen germination; IMP:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
DR CDD; cd00171; Sec7; 1.
DR Gene3D; 1.10.1000.11; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR023394; Sec7_C_sf.
DR InterPro; IPR000904; Sec7_dom.
DR InterPro; IPR035999; Sec7_dom_sf.
DR InterPro; IPR032691; Sec7_N.
DR Pfam; PF01369; Sec7; 1.
DR Pfam; PF12783; Sec7_N; 1.
DR SMART; SM00222; Sec7; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF48425; SSF48425; 1.
DR PROSITE; PS50190; SEC7; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Guanine-nucleotide releasing factor; Membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..1375
FT /note="ARF guanine-nucleotide exchange factor GNL2"
FT /id="PRO_0000420949"
FT DOMAIN 486..676
FT /note="SEC7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00189"
FT ACT_SITE 590
FT /evidence="ECO:0000250"
SQ SEQUENCE 1375 AA; 156243 MW; 40396FD1445FED4F CRC64;
MDRIAVRAKR KELGISCMLN TEVGAVLAVI RRPLSESYLS PQETDHCDSS VQQSLKSLRA
LIFNPQQDWR TIDPSVYLSP FLEVIQSDEI PASATAVALS SILKILKIEI FDEKTPGAKD
AMNSIVSGIT SCRLEKTDLV SEDAVMMRIL QVLTGIMKHP SSELLEDQAV CTIVNTCFQV
VQQSTGRGDL LQRNGRYTMH ELIQIIFSRL PDFEVRGDEG GEDSESDTDE IDMSGGYGIR
CCIDIFHFLC SLLNVVEVVE NLEGTNVHTA DEDVQIFALV LINSAIELSG DAIGQHPKLL
RMVQDDLFHH LIHYGASSSP LVLSMICSCI LNIYHFLRKF MRLQLEAFFS FVLLRVTAFT
GFLPLQEVAL EGLINFCRQP AFIVEAYVNY DCDPMCRNIF EETGKVLCRH TFPTSGPLTS
IQIQAFEGLV ILIHNIADNM DREEDEGNEE DDNNSNVIKP SPVEIHEYIP FWIDKPKEDF
ETWVDHIRVR KAQKRKLAIA ANHFNRDEKK GLEYLKYNYL VSDPLDPMAL ASFFRFTPGL
DKTMIGDYLG DPDELHLSVL RSFTHTFEFT GMNLDTALRT FLESFRLPGE SQKIERMIEA
FSERFYDQQS SDIFASKDTV HILCYSLIML NTDQHNPQVR RKMTEDEFIR NNRAINAGND
LPKEYLSELF QSIATNAFAL STHSGPVEMN PNRWIELMNR TKTTQPFSLC QFDRRIGRDM
FATIAGPSIA AVSAFFEHSD DDEVLHECVD AMISIARVAQ YGLEDILDEL IASFCKFTTL
LNPYTTPEET LFAFSHDMKP RMATLAVFTL ANTFGDSIRG GWRNIVDCLL KLRKLQLLPQ
SVIEFEINEE NGGSESDMNN VSSQDTKFNR RQGSSLMGRF SHFLALDNVE ESVALGMSEF
EQNLKVIKQC RIGQIFSKSS VLPDVAVLNL GRSLIYAAAG KGQKFSTAIE EEETVKFCWD
LIITIALSNV HRFNMFWPSY HEYLLNVANF PLFSPIPFVE KGLPGLFRVC IKILASNLQD
HLPEELIFRS LTIMWKIDKE IIETCYDTIT EFVSKIIIDY SANLHTNIGW KSVLQLLSLC
GRHPETKEQA VDALIGLMSF NASHLSQSSY AYCIDCAFSF VALRNSSVEK NLKILDLMAD
SVTMLVKWYK TASTDTANSY SPASNTSSSS SMEENNLRGV NFVHHLFLKL SEAFRKTTLA
RREEIRNRAV TSLEKSFTMG HEDLGFTPSG CIYCIDHVIF PTIDDLHEKL LDYSRRENAE
REMRSMEGTL KIAMKVLMNV FLVYLEQIVE SAEFRTFWLG VLRRMDTCMK ADLGEYGDNK
LQEVVPELLT TMIGTMKEKE ILVQKEDDDL WEITYIQIQW IAPALKDELF PDEEI
