GNPI1_GIAIN
ID GNPI1_GIAIN Reviewed; 266 AA.
AC O97439;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Glucosamine-6-phosphate isomerase 1;
DE EC=3.5.99.6;
DE AltName: Full=Glucosamine-6-phosphate deaminase 1;
DE Short=GNPDA 1;
DE Short=GlcN6P deaminase 1;
GN Name=GPI1;
OS Giardia intestinalis (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=5741;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MR4 / Polish genotype;
RA van Keulen H., Steimle P.A., Bulik D.A., Borowiak R.K., Jarroll E.L.;
RT "Cloning of two Giardia glucosamine 6-phosphate isomerase genes only one of
RT which is transcriptionally activated during encystment.";
RL Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-
CC phosphate + NH4(+); Xref=Rhea:RHEA:12172, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:57634, ChEBI:CHEBI:75989; EC=3.5.99.6;
CC -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate
CC isomerase family. {ECO:0000305}.
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DR EMBL; AF050754; AAD02508.1; -; Genomic_DNA.
DR AlphaFoldDB; O97439; -.
DR SMR; O97439; -.
DR VEuPathDB; GiardiaDB:DHA2_152372; -.
DR VEuPathDB; GiardiaDB:GL50803_008245; -.
DR eggNOG; KOG3148; Eukaryota.
DR BioCyc; MetaCyc:MON-13185; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0004342; F:glucosamine-6-phosphate deaminase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006044; P:N-acetylglucosamine metabolic process; IEA:InterPro.
DR CDD; cd01399; GlcN6P_deaminase; 1.
DR HAMAP; MF_01241; GlcN6P_deamin; 1.
DR InterPro; IPR006148; Glc/Gal-6P_isomerase.
DR InterPro; IPR004547; Glucosamine6P_isomerase.
DR InterPro; IPR018321; Glucosamine6P_isomerase_CS.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR PANTHER; PTHR11280; PTHR11280; 1.
DR Pfam; PF01182; Glucosamine_iso; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR TIGRFAMs; TIGR00502; nagB; 1.
DR PROSITE; PS01161; GLC_GALNAC_ISOMERASE; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Hydrolase.
FT CHAIN 1..266
FT /note="Glucosamine-6-phosphate isomerase 1"
FT /id="PRO_0000160127"
FT ACT_SITE 67
FT /note="Proton acceptor; for enolization step"
FT /evidence="ECO:0000250"
FT ACT_SITE 136
FT /note="For ring-opening step"
FT /evidence="ECO:0000250"
FT ACT_SITE 138
FT /note="Proton acceptor; for ring-opening step"
FT /evidence="ECO:0000250"
FT ACT_SITE 143
FT /note="For ring-opening step"
FT /evidence="ECO:0000250"
SQ SEQUENCE 266 AA; 29406 MW; 3EC301B8D868386B CRC64;
MPSIHVSKCA DPAIKLAHRI AEVVRSKPNC VLGLATGSTP IPVYQELARL HREEGLDFSQ
VRTFNLDEYA GLPPTHDQTY RFFMEEHLFS KVNIKPENVH FLNGMASDYE KECERYEQEL
KAIGPCDVWL LGIGHNGHIA FNEPGSPRDS RTRVVCLTQS TIDANARFFG NDKSKVPTKA
LSVGIATIME SREILLLATG ESKREAVTKS VKGKCETHCP ASFLHEHPHC RFYVDMDAGK
VVDNTCCQAT NQASCCGSTS CHAAKA