ID   GNPI2_GIAIN             Reviewed;         263 AA.
AC   O97440;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Glucosamine-6-phosphate isomerase 2;
DE            EC=3.5.99.6;
DE   AltName: Full=Glucosamine-6-phosphate deaminase 2;
DE            Short=GNPDA 2;
DE            Short=GlcN6P deaminase 2;
GN   Name=GPI2;
OS   Giardia intestinalis (Giardia lamblia).
OC   Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX   NCBI_TaxID=5741;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MR4 / Polish genotype;
RA   van Keulen H., Steimle P.A., Bulik D.A., Borowiak R.K., Jarroll E.L.;
RT   "Cloning of two Giardia glucosamine 6-phosphate isomerase genes only one of
RT   which is transcriptionally activated during encystment.";
RL   Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-
CC         phosphate + NH4(+); Xref=Rhea:RHEA:12172, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:57634, ChEBI:CHEBI:75989; EC=3.5.99.6;
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate
CC       isomerase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF050755; AAD02509.1; -; Genomic_DNA.
DR   RefSeq; XP_001709089.1; XM_001709037.1.
DR   AlphaFoldDB; O97440; -.
DR   SMR; O97440; -.
DR   PRIDE; O97440; -.
DR   GeneID; 5702007; -.
DR   KEGG; gla:GL50803_0010829; -.
DR   VEuPathDB; GiardiaDB:DHA2_10829; -.
DR   VEuPathDB; GiardiaDB:GL50803_0010829; -.
DR   eggNOG; KOG3148; Eukaryota.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0004342; F:glucosamine-6-phosphate deaminase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006044; P:N-acetylglucosamine metabolic process; IEA:InterPro.
DR   CDD; cd01399; GlcN6P_deaminase; 1.
DR   HAMAP; MF_01241; GlcN6P_deamin; 1.
DR   InterPro; IPR006148; Glc/Gal-6P_isomerase.
DR   InterPro; IPR004547; Glucosamine6P_isomerase.
DR   InterPro; IPR018321; Glucosamine6P_isomerase_CS.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   PANTHER; PTHR11280; PTHR11280; 1.
DR   Pfam; PF01182; Glucosamine_iso; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
DR   TIGRFAMs; TIGR00502; nagB; 1.
DR   PROSITE; PS01161; GLC_GALNAC_ISOMERASE; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Hydrolase.
FT   CHAIN           1..263
FT                   /note="Glucosamine-6-phosphate isomerase 2"
FT                   /id="PRO_0000160128"
FT   ACT_SITE        82
FT                   /note="Proton acceptor; for enolization step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        151
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        153
FT                   /note="Proton acceptor; for ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        158
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   263 AA;  28768 MW;  902C356194436A88 CRC64;
     MTVCVAQAER GASELESKAM VKKSNDAALL VAHRIAEVVR SKPNCVLGLA TGSTPIPVYQ
     ELARLHREEG LDFSQVRTFN LDEYAGLPPT HDQSYRFFME EHLFSKVNIK PENVHFLSGL
     AIRAEDECIR YENALQTIGP CDVWLLGIGH NGHIAFNEPG SPRDSRTRVV CLTQSTIDAN
     ARFFGNDKSK VPTKALSVGI ATIMESREIL LLATGESKCE AVTKAIVGEP TNAVPASLLQ
     RHPRCCFYVD EAAGAEVAET VTE