ID   GNPI2_XENTR             Reviewed;         275 AA.
AC   A4IHW6;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Glucosamine-6-phosphate isomerase 2;
DE            EC=3.5.99.6;
DE   AltName: Full=Glucosamine-6-phosphate deaminase 2;
DE            Short=GNPDA 2;
DE            Short=GlcN6P deaminase 2;
GN   Name=gnpda2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-
CC         phosphate + NH4(+); Xref=Rhea:RHEA:12172, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:57634, ChEBI:CHEBI:75989; EC=3.5.99.6;
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate
CC       isomerase family. {ECO:0000305}.
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DR   EMBL; BC135724; AAI35725.1; -; mRNA.
DR   RefSeq; NP_001096324.1; NM_001102854.1.
DR   AlphaFoldDB; A4IHW6; -.
DR   SMR; A4IHW6; -.
DR   STRING; 8364.ENSXETP00000060035; -.
DR   DNASU; 100124908; -.
DR   GeneID; 100124908; -.
DR   KEGG; xtr:100124908; -.
DR   CTD; 132789; -.
DR   Xenbase; XB-GENE-983383; gnpda2.
DR   eggNOG; KOG3148; Eukaryota.
DR   InParanoid; A4IHW6; -.
DR   OrthoDB; 1425290at2759; -.
DR   Reactome; R-XTR-70171; Glycolysis.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000008437; Expressed in testis and 12 other tissues.
DR   ExpressionAtlas; A4IHW6; differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004342; F:glucosamine-6-phosphate deaminase activity; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; IBA:GO_Central.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006043; P:glucosamine catabolic process; IBA:GO_Central.
DR   GO; GO:0006046; P:N-acetylglucosamine catabolic process; IBA:GO_Central.
DR   GO; GO:0019262; P:N-acetylneuraminate catabolic process; IBA:GO_Central.
DR   CDD; cd01399; GlcN6P_deaminase; 1.
DR   HAMAP; MF_01241; GlcN6P_deamin; 1.
DR   InterPro; IPR006148; Glc/Gal-6P_isomerase.
DR   InterPro; IPR004547; Glucosamine6P_isomerase.
DR   InterPro; IPR018321; Glucosamine6P_isomerase_CS.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   PANTHER; PTHR11280; PTHR11280; 1.
DR   Pfam; PF01182; Glucosamine_iso; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
DR   TIGRFAMs; TIGR00502; nagB; 1.
DR   PROSITE; PS01161; GLC_GALNAC_ISOMERASE; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Coiled coil; Cytoplasm; Hydrolase;
KW   Reference proteome.
FT   CHAIN           1..275
FT                   /note="Glucosamine-6-phosphate isomerase 2"
FT                   /id="PRO_0000343208"
FT   COILED          103..131
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        72
FT                   /note="Proton acceptor; for enolization step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        141
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        143
FT                   /note="Proton acceptor; for ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        148
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   275 AA;  30971 MW;  F043EB98149BFC8D CRC64;
     MRLVILDDYA LASEWAAKYI CNRIIQFSPG PDKYFTLGLP TGSTPLGCYK KLIEYHKSGD
     LSFKYVKTFN MDEYVGLPRD HPESYHSYMW NNFFKHIDID PSNAHILDGN ASDLQAECED
     FERKIKEAGG IELFVGGIGP DGHIAFNEPG SSLVSRTRLK TLAMDTILAN AKYFDGDLSK
     VPTMALTVGV GTVMDAREVM ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTIFVCD
     EDATLELRVK TVKYFKGLMH VHNRLVDPLH SMKKN